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csmc.py
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csmc.py
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"""
A reference implementation of the Combinatorial Sequential Monte Carlo algorithm
for Bayesian Phylogenetic Inference from the publication
Liangliang Wang, Alexandre Bouchard-Côté & Arnaud Doucet (2015)
Bayesian Phylogenetic Inference Using a Combinatorial Sequential Monte Carlo Method,
Journal of the American Statistical Association, 110:512, 1362-1374, DOI: 10.1080/01621459.2015.1054487
"""
import numpy as np
import random
from scipy.stats import lognorm
import scipy.linalg as spl
from scipy.linalg import expm
import matplotlib.pyplot as plt
import pandas as pd
import pdb
from copy import deepcopy
from functools import reduce
import operator as op
import networkx as nx
from networkx.drawing.nx_agraph import graphviz_layout
class Node:
"""
Defines a Node object for visualizing phylogeny
"""
def __init__(self, data):
# Subedges must be a list of edges (or empty list)
self.data = data
self.subnodes = []
self.parent = None
self.edge_before = ''
def del_subnodes(self, d):
for n in self.subnodes:
if n.data == d:
self.subnodes.remove(n)
def print_node(self):
s = 'Node ' + self.data + ' with subnodes: '
for n in self.subnodes:
s += n.data + ' '
s += '; edge before is ' + self.edge_before
print(s)
class Vertex:
def __init__(self, id=None, data=None):
self.id = id
self.data = data
self.left = None
self.right = None
self.left_branch = None
self.right_branch = None
self.is_root = True
self.data_done = False
class Graph:
"""
Defines a Graph object for visualizing phylogeny
"""
def __init__(self):
self.node_dict = {}
self.num_nodes = 0
def add_node(self, node):
self.num_nodes += 1
self.node_dict[node.data] = node
return node
def get_node(self, data):
if data in self.node_dict:
return self.node_dict[data]
else:
return None
def del_node(self, data):
try:
for d in self.node_dict:
node = self.get_node(d)
node.del_subnodes(data)
del self.node_dict[data]
self.num_nodes -= 1
return None
except KeyError:
raise Exception("Node %s does not exist" % data)
def contains(self, data):
return data in self.node_dict
def get_nodes(self):
return list(self.node_dict.values())
def get_nodes_data(self):
return list(self.node_dict.keys())
# For visualizations
def build_nx_graph(self):
# Add nodes and edges to build graph
G_nx = nx.DiGraph()
for key in self.get_nodes_data():
G_nx.add_node(key)
for node in self.get_nodes():
for subnode in node.subnodes:
G_nx.add_edge(node.data, subnode.data)
return G_nx
def draw(self, prob):
# plotting tools. Not used here.
G_nx = self.build_nx_graph()
plt.figure(figsize=(10,10))
pos = nx.kamada_kawai_layout(G_nx)
nx.draw_networkx(G_nx, pos=pos, with_labels=True, fontsize=4,width=3.8,node_color='r',edge_color='brown')
plt.title("Sampled Geneaology", fontsize=14)
plt.xlabel("Prob %1.5f " % prob)
plt.show()
def __iter__(self):
return iter(self.node_dict.values())
class CSMC:
"""
CSMC takes as input a dictionary (datadict) with two keys:
taxa: a list of n strings denoting taxa
genome_NxSxA: a 3 tensor of genomes for the n taxa one hot encoded
"""
def __init__(self, datadict):
self.n = len(datadict['taxa'])
self.taxa = datadict['taxa']
self.genome_NxSxA = datadict['genome']
self.s = len(self.genome_NxSxA[0])
# self.Qmatrix = np.array([[-3.,1.,1.,1.],
# [1.,-3.,1.,1.],
# [1.,1.,-3.,1.],
# [1.,1.,1.,-3.]])
self.Qmatrix = np.array([[-1.,.25,.5,.25],
[.25,-1.,.25,.5],
[.5,.25,-1.,.25],
[.25,.5,.25,-1.]])/10
self.Pmatrix = spl.expm(self.Qmatrix)
self.prior = np.ones(self.Qmatrix.shape[0])/self.Qmatrix.shape[0]
#@staticmethod
def ncr(self, n, r):
# Compute combinatorial term n choose r
r = min(r, n-r)
numer = reduce(op.mul, range(n, n-r, -1), 1)
denom = reduce(op.mul, range(1, r+1), 1)
return numer/denom
#@staticmethod
def create_node_sampler(self, jump_chain, i):
"""
This defines the sample space of nodes at which coalescent events can happen.
The sample space is defined in the list variable 'result'
REVISIT ME. This is no longer necessary...
"""
result = []
for key in jump_chain[i]:
if len(jump_chain[i][key]) > 1:
for p in range(len(jump_chain[i][key])):
result.append(key)
return result
def build_tree(self, particle1, particle2, particle_coalesced, j):
"""
Updates two lists graph_repn_data_K and graph_repn_nodes_K
that are needed to plot a phylogeny using the Graph object
"""
#pdb.set_trace()
n3 = Node(particle_coalesced)
if particle1 not in self.graph_repn_data_K[j]:
n1 = Node(particle1)
self.graph_repn_data_K[j].append(particle1)
self.graph_repn_nodes_K[j].append(n1)
else:
n1 = self.graph_repn_nodes_K[j][self.graph_repn_data_K[j].index(particle1)]
n1.parent = n3
n3.subnodes.append(n1)
if particle2 not in self.graph_repn_data_K[j]:
n2 = Node(particle2)
self.graph_repn_data_K[j].append(particle2)
self.graph_repn_nodes_K[j].append(n2)
else:
n2 = self.graph_repn_nodes_K[j][self.graph_repn_data_K[j].index(particle2)]
n2.parent = n3
n3.subnodes.append(n2)
self.graph_repn_data_K[j].append(particle_coalesced)
self.graph_repn_nodes_K[j].append(n3)
def build_graph(self, G, master_node):
"""
Constructs a graph for visualizing phylogeny
"""
if master_node.subnodes == []:
G.add_node(master_node)
return G
G.add_node(master_node)
for n in master_node.subnodes:
G = self.build_graph(G, n)
return G
#@staticmethod
def resample(self, weights, jump_chain_K, i):
"""
Resamples partial states (particles) based on importance weights
"""
#pdb.set_trace()
K = weights.shape[0]
importance_weights = np.exp(weights[:,i])
norm = np.sum(importance_weights)
indices = np.random.choice(K, K, p = importance_weights/norm, replace=True)
jump_chain_K = jump_chain_K[indices]
return jump_chain_K
#@staticmethod
def sort_string(self, s):
lst = s.split('+')
lst = sorted(lst)
result = '+'.join(lst)
return result
def extend_partial_state(self, jump_chain_KxN, j, i):
"""
Forms a partial state by sampling two nodes from the proposal distribution
"""
# Set c[n-1] = c[n], copying our dictionary representing the particle set
jump_chain_KxN[j, i + 1] = deepcopy(jump_chain_KxN[j, i])
# Sample two posets
sample = random.sample(jump_chain_KxN[j,i][0], 2)
q2 = 1 / self.ncr(len(jump_chain_KxN[j,i][0]), 2)
particle1 = sample[0]
particle2 = sample[1]
particle_coalesced = self.sort_string(particle1 + '+' + particle2)
jump_chain_KxN[j,i+1][0].remove(particle1)
jump_chain_KxN[j,i+1][0].remove(particle2)
jump_chain_KxN[j,i+1][0].append(particle_coalesced)
# Sample from branch length proposal
bl1, bl2 = 2, 2
return particle1, particle2, particle_coalesced, bl1, bl2, q2, jump_chain_KxN
def get_internal_nodes(self,root):
"""
Collects all internal nodes (ancestral or latent variables) for likelihood computation
May not be necessary...
"""
#pdb.set_trace()
q = []
q.append(root)
nodes = []
while (len(q)):
curr = q[0]
q.pop(0)
isInternal = 0
if (curr.left):
isInternal = 1
if not curr.left.data_done:
q.append(curr.left)
if (curr.right):
isInternal = 1
if not curr.left.data_done:
q.append(curr.right)
if (isInternal):
nodes.append(curr)
# print(curr.id, end = " ")
# Make sure that node ordering is such that any child is placed before its parent
return nodes[::-1]
def pass_messages(self,internal_nodes):
#pdb.set_trace()
# Pass messages from leaf nodes to root
for node in internal_nodes:
if not node.data_done:
node.data = self.conditional_likelihood(node.left, node.right, node.left_branch, node.right_branch)
node.data_done = True
def conditional_likelihood(self, left, right, left_branch, right_branch):
#pdb.set_trace()
# Computes the conditional likelihood using the formula above
# Matrix exponentiation here is probably inefficient
left_Pmatrix = spl.expm(self.Qmatrix * left_branch)
right_Pmatrix = spl.expm(self.Qmatrix * right_branch)
left_prob = np.dot(left.data, left_Pmatrix)
right_prob = np.dot(right.data, right_Pmatrix)
likelihood = np.multiply(left_prob, right_prob)
return likelihood
def compute_tree_likelihood(self, prior, root):
'''
multiply probabilities over all the sites
'''
tree_likelihood = np.dot(prior, root.data.T)
return tree_likelihood
def compute_log_conditional_likelihood(self, v):
#pdb.set_trace()
internal_nodes = self.get_internal_nodes(v)
self.pass_messages(internal_nodes)
tree_likelihood = self.compute_tree_likelihood(self.prior, v)
loglik = 0
for i in range(self.s):
loglik += np.log(tree_likelihood[i])
return loglik
def overcounting_correct(self, vertex_dict):
rho = 0
for key in vertex_dict:
if vertex_dict[key].is_root and vertex_dict[key].left is not None:
rho += 1
return 1/rho
def get_tree_prob(self, vertex_dicts, weights_KxNm1, K):
trees = []
for dic in vertex_dicts:
trees.append(dic.keys())
tree_probabilities = []
for i in range(len(trees)):
tree = trees[i]
tree_probabilities.append(0)
for k in range(K):
if tree == trees[k]:
tree_probabilities[i] += weights_KxNm1[k,-1]
tree_probabilities[i] /= K
tree_probabilities /= 1/K*sum(weights_KxNm1[:,-1])
tree_probabilities = list(tree_probabilities)
return tree_probabilities, trees
def compute_norm(self, weights_KxNm1, K):
norm = 1
for i in range(1,self.n-1):
norm *= 1/K*sum(weights_KxNm1[:,i])
return norm
def sample_phylogenies(self, K, resampling=False, showing=True):
n = self.n
# Represent a single jump_chain as a list of dictionaries
jump_chain = [{} for i in range(n)]
jump_chain[0][0] = self.taxa
jump_chain_KxN = np.array([jump_chain] * K) # KxN matrix of jump_chain dictionaries
log_weights_KxNm1 = np.zeros([K, n-1])
weights_KxNm1 = np.zeros([K, n-1]) + 1
# These need better names that describe what is happening here
self.graph_repn_data_K = [[] for i in range(K)]
self.graph_repn_nodes_K = [[] for i in range(K)]
log_likelihood = np.zeros([K,n-1])
log_likelihood_tilda = np.zeros(K)+1
vertex_dicts = [{} for k in range(K)]
for j in range(K):
for i in range(n):
vertex_dicts[j][self.taxa[i]] = Vertex(id=self.taxa[i],data=self.genome_NxSxA[i])
# Iterate over coalescent events
for i in range(n - 1):
# Resampling step
if resampling and i > 0:
jump_chain_KxN[:,i-1] = self.resample(log_weights_KxNm1, jump_chain_KxN[:,i-1], i-1)
# Iterate over partial states
for k in range(K):
# Sample from last step
if i > 0:
log_likelihood_tilda[k] = 0
idx = random.randint(0,K-1)
for key in vertex_dicts[idx]:
if vertex_dicts[idx][key].is_root:
log_likelihood_tilda[k] \
+= self.compute_log_conditional_likelihood(vertex_dicts[idx][key])
# Extend partial states
particle1, particle2, particle_coalesced, bl1, bl2, q, jump_chain_KxN \
= self.extend_partial_state(jump_chain_KxN, k, i)
# Save partial set and branch length data
vertex_dicts[k][particle_coalesced] = Vertex(id=particle_coalesced, data=None)
vertex_dicts[k][particle_coalesced].left = vertex_dicts[k][particle1]
vertex_dicts[k][particle_coalesced].right = vertex_dicts[k][particle2]
vertex_dicts[k][particle_coalesced].left_branch = bl1
vertex_dicts[k][particle_coalesced].right_branch = bl2
vertex_dicts[k][particle1].is_root = False
vertex_dicts[k][particle2].is_root = False
# Build tree
self.build_tree(particle1, particle2, particle_coalesced, k)
for k in range(K):
# Compute log conditional likelihood across genome for a partial state
log_likelihood[k,i] = 0
for key in vertex_dicts[k]:
if vertex_dicts[k][key].is_root:
log_likelihood[k,i] \
+= self.compute_log_conditional_likelihood(vertex_dicts[k][key])
# Overcounting correction
v = self.overcounting_correct(vertex_dicts[k])
# Compute the importance weights
if i > 0:
log_weights_KxNm1[k, i] = log_likelihood[k,i] - log_likelihood_tilda[k] + np.log(v) - np.log(q)
weights_KxNm1[k, i] = np.exp(log_weights_KxNm1[k, i])
vertex_dicts_laststep = deepcopy(vertex_dicts)
print('Computation in progress: step ' + str(i+1))
# End of iteration
print(log_likelihood)
#print(log_likelihood_tilda)
#print(weights_KxNm1)
tree_probabilities, trees = self.get_tree_prob(vertex_dicts, weights_KxNm1, K)
#print(tree_probabilities)
norm = self.compute_norm(weights_KxNm1, K)
print(norm)
selected_idx = tree_probabilities.index(max(tree_probabilities))
print(trees[selected_idx])
# plt.hist(tree_probabilities)
# plt.xlabel('Posterior probability')
# plt.ylabel('Number of generated trees')
# plt.show()
# np.savetxt('tree_prob.csv', tree_probabilities, delimiter=',')
# pdb.set_trace()
G = self.build_graph(Graph(), self.graph_repn_nodes_K[selected_idx][-1])
if showing:
G.draw(tree_probabilities[selected_idx])
return log_weights_KxNm1, tree_probabilities, norm, G
if __name__ == "__main__":
real_data_corona = False
real_data_1 = False
real_data_2 = False
simulate_data = False
load_strings = True
Alphabet_dir = {'A': [1, 0, 0, 0],
'C': [0, 1, 0, 0],
'G': [0, 0, 1, 0],
'T': [0, 0, 0, 1]}
alphabet_dir = {'a': [1, 0, 0, 0],
'c': [0, 1, 0, 0],
'g': [0, 0, 1, 0],
't': [0, 0, 0, 1]}
alphabet = np.array([[1, 0, 0, 0], [0, 1, 0, 0], [0, 0, 1, 0], [0, 0, 0, 1]])
genome_strings = ['ACTTTGAGAG','ACTTTGACAG','ACTTTGACTG','ACTTTGACTC']
#genome_strings = ['AAAAAA','CCCCCC','TTTTTT','GGGGGG']
# a = ''
# for i in range(150):
# a += 'A'
# genome_strings = [a,a,a,a]
def simulateDNA(nsamples, seqlength, alphabet):
genomes_NxSxA = np.zeros([nsamples, seqlength, alphabet.shape[0]])
for n in range(nsamples):
genomes_NxSxA[n] = np.array([random.choice(alphabet) for i in range(seqlength)])
return genomes_NxSxA
def form_dataset_from_strings(genome_strings, alphabet_dir):
#pdb.set_trace()
genomes_NxSxA = np.zeros([len(genome_strings),len(genome_strings[0]),len(alphabet_dir)])
for i in range(genomes_NxSxA.shape[0]):
for j in range(genomes_NxSxA.shape[1]):
genomes_NxSxA[i,j] = alphabet_dir[genome_strings[i][j]]
taxa = ['S' + str(i) for i in range(genomes_NxSxA.shape[0])]
datadict = {'taxa': taxa,
'genome': genomes_NxSxA}
return datadict
dim = 4
if simulate_data:
data_NxSxA = simulateDNA(3, 10, alphabet)
#print("Simulated genomes:\n", data_NxSxA)
taxa = ['S' + str(i) for i in range(data_NxSxA.shape[0])]
#print(taxa)
datadict = {'taxa': taxa,
'genome': data_NxSxA}
if load_strings:
datadict = form_dataset_from_strings(genome_strings, Alphabet_dir)
if real_data_corona:
datadict = pd.read_pickle('tencovid.p')
dim = 6
if real_data_1:
genome_strings = \
['aaccctgttatttccacatgccaacaatcccaacag',
'aactctgttatttccacatgccaacaatcccaacag',
'aaatctgtgttgtctaaatgtcagttatttcagtta',
'aaagctattatttaaaaatataaattatctcaatta',
'aacactgttatttctaaatatcacttttcccaattg']
datadict = form_dataset_from_strings(genome_strings, alphabet_dir)
datadict['taxa'] = ['human', 'gibbon','guinea pig', 'aardvark', 'armadillo']
if real_data_2:
# Primates
# block 19, 20, 32, 38, 42, 45, 47, 52, 53, 54, 57, 74, 78, 89, 92, 202, 223, 228, 239, 286, 304, 309, 346
genome_strings = \
['taatggaataacacctttgctatgttatccaaacaatattagtcctttttcttctcttgtcgcccagccagagggcaatggtgggatctcggctcactgagacctctgcctcccagttcaagttacaggcacccgccaggctggtctcgaactgctgacctcaggtgatccacccaccttggcctccgaaagtgccgggattataggcgtgagccaccgcaccacctagcttgtatcgaacaaagggaataaaaaatgtatggatcaaggctcatgtacacaagatccaaattatccaccatccaggataatattttttgg',
'gaatggaataacacctttgctatgttatccaaacaatattagtcctttttcttctcttgtcgcccagccagagggcaatggtgggatctccgctcactgagacctctgcctcccagttcaagttacaggcacccgccaggctggtctcgaactgctgacctcaggtgaaccacccaccttggcctccgaaagtgccgggattataggcgtgagccaccgcaccacctagcttgtatcgaacaaagggaataaaaaatgtatggatcaaggctcatgtacacaagatccaaattatccaccatccaggataatattttttgg',
'taatggaataacacctttgctatgttatccaaacaatattagtccttttttttctcttgtcacccagccagagggcaatggcgggatctcggctcactgagacctctgcctcccagttcaagctacaggcacccgccaggctggtcttgaactgctgacctcaggtgatccacccaccttggcctccaaaagtgccgggattataggtgtgagccaccgcaccacctagcttgtatcgaactaagggaataaaaaatgtatggatcaaggctcatgtacacaagatccaaattatccaccatccaggataatatttttcgg',
'taatggaataacacctttgctatgttatccaaacaatattagtcctattttttctcttgtcacccagccagagggcaatggtgggatctcggctcactgcgacctctgcctcccagttcaagctacaggcacccgccaggctgggctccaactgctgacctcaggtgatccacccatcttggcctccgaaagtgccgggattacaggcgtgagccaccgcactgcctagtttgtatcgaacaaagggaatataaaatgtatgattcaaggctcatgtacacaagatccaaattatcccccatccaggatagtattttacgg',
'taatggaataacacctttgctatgttattcaaacaatattagtcctattttttctcttgttgcccagctggagggcaatggcgggatctcggctcgctgccacctctgcctcccagttcaggctacaggcacctgccatgctgttcctgaactgctgacctcaggtgatccacctaccttggcctccaaaagtgccgggattacaggcgtgagccaccgcactgcctagtttgtattgaacaaagggaatataaaatgtatgaatcaaggctcatgtacacaagatccaaattatccaccatccaggataatattttatgg',
'taacagaataacacctttactatgttatctaaataatatttgtcctattttttctcttgtcacccagctggaaagcaatggcgggacctcagctcactgcaacctctgcctcccagttcaagctataggcatctgccaggctggtctcgaactgctgacctcaggtgatccacccgccttggcctcccaaagcgctgggattgtaggcatgagccaccccgccacctagtttgtatagaatataggagatacaaaatgtatgaatcaaggctgacgtatacacgatccaaattatcccccacccaggacaatattttctga',
'taacagaataacacctttgctatgttatctaaataatatttgtcctattttttctcttgtcacccagctggaaagcaatggcgggacctcagctcactgcaacctctgcctcccagttcaagctacaggcatctgccaggctggtctcgaactgctgacctcaggtgatccacccgccttggcctcccaaagcgctgggattgtaggcatgagccaccccgccacctagtttgtatagaatataggagatacaaaatgtatgaatcaaggctgacgtatacacgatccaaattatcccccacccaggacaatattttctga',
'taacagaataacacttttgctatgttatctaaataatatttgtcctatttcttctcttgtcgcccagctggaaggcaatggcgggacctcagctcactgcaacctctgcctcccagttcaagctacaggcatctgccaggctggtctagaactgctgacctcaggtgatccacccgccttggcctcccaaagtgctggaattgcaggcatgagccaccccgccacctagtttgtatagaatataggagatacaaaatgtatgaatcaaggctgacgtccacacgatccaaattatcccccacccaggacaatattttctga',
'taacagaataacacctttgctatgttatctaaataatatttgtcctattttttctcttgtcgcccagctggtgggcaatggcggaatctcggctcaatgcaacctctgcctcccagttcaagctacaggcatctgtcaggctggtctcaaactgctgacctcaggtgatccacccgccttggcctcccaaagtgctgggattacaggcacgacccaccccgccacctagtttgtatagaatagaggagatacaaaatgtatgaatccaggctgacgtacacacgatccaaattatcccccacccaggacaatattttctga']
datadict = form_dataset_from_strings(genome_strings, alphabet_dir)
datadict['taxa'] = ['human','chimp','gorilla','oranguta','gibbon','rhesus','macaque','baboon','greenmonkey']
csmc = CSMC(datadict)
if dim==6:
csmc.Qmatrix = np.array([[-5.,1.,1.,1.,1.,1.],
[1.,-5.,1.,1.,1.,1.],
[1.,1.,-5.,1.,1.,1.],
[1.,1.,1.,-5.,1.,1.],
[1.,1.,1.,1.,-5.,1.],
[1.,1.,1.,1.,1.,-5.]])
csmc.Pmatrix = spl.expm(csmc.Qmatrix)
csmc.prior = np.ones(csmc.Qmatrix.shape[0])/csmc.Qmatrix.shape[0]
log_weights, tree_probs, norm, G = csmc.sample_phylogenies(8, resampling=False, showing=True)