Update README.md

README.md

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 Serum was obtained from a single patient during the 2013 Dengue virus serotype 1 outbreak in Martin County in Florida. Patient serum (250uL) was inoculated into a T-25 flask of C6/36 cells, incubated @ RT for 20 days, blind passaged, and incubated for another 31 days. After this first passage, the sample was split into two and one sample (DV1_Vero) was subject to one additional passage in Vero cells, and the other sample (DV1_c6/36) was subject to one additional passage in C6/36 cells.
 
-**Sequencing**
+#### Sequencing
 
 RNA was extracted and metagenomic sequencing was performed on the Illumina MiSeq after rRNA depletion following the protocol in Matranga et al. (Genome Biol 2014). 
 
-**Samples**
+#### Samples
 
 DV1_vero	One passage in C6/36 cells, one additional passage in Vero cells
 DV1_c6/36	One passage in C6/36 cells, one additional passage in C6/36 cells
 
 Both consensus sequences were identical - this was confirmed by additional sequencing of biological replicates (separate RNA extractions)
 
-**Alignment**
+#### Alignment
 
 ORF-complete DENV-1 genomes were downloaded from ViPR and an alignment together with the Martin County DENV-1 sequence was created using MAFFT.
 
-**Tree**
+#### Tree
 
 A tree was created based on the MAFFT multiple sequence alignment using RAxML.
 
-**References**
+#### References
 
 [Enhanced methods for unbiased deep sequencing of Lassa and Ebola RNA viruses from clinical and biological samples](https://www.ncbi.nlm.nih.gov/pubmed/25403361).