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Modelling and Analysis of Real-Time PCR Data

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qpcR: Modelling and Analysis of Real-Time PCR Data (R-package)

qpcR is an R package that can analyze sigmoidal qPCR (quantitative polymerase chain reaction) data by fitting various sigmoidal, mechanistic, exponential and spline models to the raw data. From the fitted curves, various location indices, also called Cq values, as well as qPCR efficiencies E can be extracted and employed for ratio calculations, and this under an error propagation regime. This packages houses many published algorithms that other qPCR analysis softwares do not provide.

Installation

You can install the latest development version of the code using the devtools R package.

# Install devtools, if you haven't already.
install.packages("devtools")
library(devtools)
install_github("anspiess/qpcR")
source("https://install-github.me/anspiess/qpcR")

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Modelling and Analysis of Real-Time PCR Data

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