Glutton is a novel tool to process transcriptome assemblies for downstream evolutionary analyses. Glutton takes as input a set of fragmented, possibly erroneous transcriptome assemblies. Utilising phylogeny-aware alignment and reference data from a closely related species, it reconstructs one transcript per gene, finds orthologous sequences and produces accurate multiple alignments of coding sequences.
The method is described in a preprint at https://www.biorxiv.org/content/10.1101/077511v1. Example data for testing the method is available at https://etsin.fairdata.fi/dataset/b518de29-6b93-41f4-8211-e7207ffd251b.
