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@roryk roryk released this 06 Dec 01:48
· 749 commits to master since this release
  • Fix for get VEP cache.
  • Support Picard's new syntax for ReorderSam (REFERENCE -> SEQUENCE_DICTIONARY).
  • Remove mitochondrial reads from ChIP/ATAC-seq calling.
  • Add documentation describing ATAC-seq outputs.
  • Add ENCODE library complexity metrics for ATAC/ChIP-seq to MultiQC report
    (see https://www.encodeproject.org/data-standards/terms/#library for a description of the metrics)
  • Add STAR sample-specific 2-pass. This helps assign a moderate number of reads per genes. Thanks
    to @naumenko-sa for the intial implementation and push to get this going.
  • Index transcriptomes only once for pseudo/quasi aligner tools. This fixes race conditions that
    can happen.
  • Add --buildversion option, for tracking which version of a gene build was used. This is used
    during bcbio_setup_genome.py. Suggested formats are source_version, so Ensembl_94,
    EnsemblMetazoa_25, FlyBase_26, etc.
  • Sort MACS2 bedgraph files before compressing. Thanks to @LMannarino for the suggestion.
  • Check for the reserved field sample in RNA-seq metadata and quit with a useful error message.
    Thanks to @marypiper for suggesting this.
  • Split ATAC-seq BAM files into nucleosome-free and mono/di/tri nucleosome files, so we can call
    peaks on them separately.
  • Call peaks on NF/MN/DN/TN regions separately for each caller during ATAC-seq.
  • Allow viral contamination to be assasyed on non tumor/normal samples.
  • Ensure EBV coverage is calculated when run on genomes with it included as a contig.