Add Homologs
If a HomologsStats.txt file is found by BACNET and it contains the information on the homologs for your database species you can skip this part.
Otherwise start by clicking on Create Blast database for Homolog search. It will automatically create a Temp/BLASTDB folder, copy all genomes files and create a verification file.
Click on Create BlastP script. As stated on the console you need to update the path of your blast bin folder and your data path on your computer.
- Open in Eclipse the page bacnet.scripts > src > bacnet > scripts > database > HomologCreation.java and change the appropriate lines (PATH_BLAST for the correct OS).
- Update your data path (PATH_SCRIPT) to match the path given to you in BACNET console.
Check the following screenshot for the paths to change.
After applying those changes click again on Create BlastP script. Through command line launch the created BlastP scripts. All scripts are stored in /GenomeNCBI/Temp/Threads/Commands/.
To verify that BlastP scripts were run correctly click on Check BlastP script.
To create the homologs table click on Create Homolog tables and check in Eclipse console that no errors or unfound files were mentionned. If no problem appeared click on Add Homologs information to Genomes to finish adding the found homologs to your database.
If everything went well you can now deploy "bacnet.e4.rap" by running it on Eclipse. Go to Genes Information page on the Home page. Select a gene, select Homologs panel, you should see the 2 homologs of your protein in the other genomes.
Now pursue on the Biological Conditions panel.