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TurboID

TurboID Mass Spectrometry Analysis

  1. Run parseproteinfa.sh.

    To parse the protein fasta sequences. The script will take the longest isoform of protein for each gene. It will also output the wormbase gene ID and protein sequence.

  2. Run iupred_analysis.sh

    This script finds the per base iupred score for each protein in a given fasta and outputs the results file in the format "protein".iupred.txt

  3. Run process_iupred.py.

    This will process output from iupred_analysis.sh. Takes total iupred score for protein and divides by protein length to yeild mean disorder of protein

  4. Used the turboid-analysis.R script for statistical analysis and plotting

All other scripts were used in other analyses throughout the report.

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TurboID Mass Spectrometry Analysis

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