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DESCRIPTION
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DESCRIPTION
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Package: TAPseq
Type: Package
Title: Targeted scRNA-seq primer design for TAP-seq
Version: 1.17.0
Authors@R: c(
person(c("Andreas", "R."), "Gschwind", email = "andreas.gschwind@stanford.edu",
role = c("aut", "cre"), comment = c(ORCID = "0000-0002-0769-6907")),
person("Lars", "Velten", email = "lars.velten@crg.eu", role = "aut",
comment = c(ORCID = "0000-0002-1233-5874")),
person(c("Lars", "M."), "Steinmetz", role = "aut"))
Description: Design primers for targeted single-cell RNA-seq used by TAP-seq. Create sequence
templates for target gene panels and design gene-specific primers using Primer3. Potential
off-targets can be estimated with BLAST. Requires working installations of Primer3 and BLASTn.
URL: https://github.com/argschwind/TAPseq
BugRepots: https://github.com/argschwind/TAPseq/issues
biocViews: SingleCell, Sequencing, Technology, CRISPR, PooledScreens
License: MIT + file LICENSE
Encoding: UTF-8
RoxygenNote: 7.2.3
VignetteBuilder: knitr
Depends: R (>= 4.0.0)
Imports:
methods,
GenomicAlignments,
GenomicRanges,
IRanges,
BiocGenerics,
S4Vectors (>= 0.20.1),
GenomeInfoDb,
BSgenome,
GenomicFeatures,
Biostrings,
dplyr,
tidyr,
BiocParallel
Suggests:
testthat,
BSgenome.Hsapiens.UCSC.hg38,
knitr,
rmarkdown,
ggplot2,
Seurat,
glmnet,
cowplot,
Matrix,
rtracklayer,
BiocStyle
SystemRequirements:
Primer3 (>= 2.5.0),
BLAST+ (>=2.6.0)