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mmvec
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mmvec
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#!/usr/bin/env python3
import os
import time
import click
import datetime
from tqdm import tqdm
import pandas as pd
import numpy as np
from biom import load_table, Table
from biom.util import biom_open
from skbio import OrdinationResults
from skbio.stats.composition import clr, centralize, closure
from skbio.stats.composition import clr_inv as softmax
from scipy.stats import entropy, spearmanr
from scipy.sparse import coo_matrix
from scipy.sparse.linalg import svds
import tensorflow as tf
from tensorflow.contrib.distributions import Multinomial, Normal
from mmvec.multimodal import MMvec
from mmvec.util import split_tables, format_params
import matplotlib.pyplot as plt
@click.group()
def mmvec():
pass
@mmvec.command()
@click.option('--microbe-file',
help='Input microbial abundances')
@click.option('--metabolite-file',
help='Input metabolite abundances')
@click.option('--metadata-file', default=None,
help='Input sample metadata file')
@click.option('--training-column',
help=('Column in the sample metadata specifying which '
'samples are for training and testing.'),
default=None)
@click.option('--num-testing-examples',
help=('Number of samples to randomly select for testing'),
default=10)
@click.option('--min-feature-count',
help=('Minimum number of samples a microbe needs to be observed '
'in order to not filter out'),
default=10)
@click.option('--epochs',
help='Number of epochs to train', default=10)
@click.option('--batch-size',
help='Size of mini-batch', default=32)
@click.option('--latent-dim',
help=('Dimensionality of shared latent space. '
'This is analogous to the number of PC axes.'),
default=3)
@click.option('--input-prior',
help=('Width of normal prior for input embedding. '
'Smaller values will regularize parameters towards zero. '
'Values must be greater than 0.'),
default=1.)
@click.option('--output-prior',
help=('Width of normal prior for input embedding. '
'Smaller values will regularize parameters towards zero. '
'Values must be greater than 0.'),
default=1.)
@click.option('--arm-the-gpu', is_flag=True,
help=('Enables GPU support'),
default=False)
@click.option('--learning-rate',
help=('Gradient descent decay rate.'),
default=1e-5)
@click.option('--beta1',
help=('Gradient decay rate for first Adam momentum estimates'),
default=0.9)
@click.option('--beta2',
help=('Gradient decay rate for second Adam momentum estimates'),
default=0.95)
@click.option('--clipnorm',
help=('Gradient clipping size.'),
default=10.)
@click.option('--checkpoint-interval',
help=('Number of seconds before a storing a summary.'),
default=1000)
@click.option('--summary-interval',
help=('Number of seconds before a storing a summary.'),
default=1000)
@click.option('--summary-dir', default='summarydir',
help='Summary directory to save cross validation results.')
@click.option('--embeddings-file', default=None,
help=('Path to save the embeddings learned from the model. '
'If this is not specified, then this will be saved under '
'`--summary-dir`.'))
@click.option('--ranks-file', default=None,
help=('Path to save the ranks learned from the model. '
'If this is not specified, then this will be saved under '
'`--summary-dir`.'))
@click.option('--ordination-file', default=None,
help=('Path to save the ordination learned from the model. '
'If this is not specified, then this will be saved under '
'`--summary-dir`.'))
def paired_omics(microbe_file, metabolite_file,
metadata_file, training_column,
num_testing_examples, min_feature_count,
epochs, batch_size, latent_dim,
input_prior, output_prior, arm_the_gpu,
learning_rate, beta1, beta2, clipnorm,
checkpoint_interval, summary_interval,
summary_dir, embeddings_file, ranks_file, ordination_file):
microbes = load_table(microbe_file)
metabolites = load_table(metabolite_file)
if metadata_file is not None:
metadata = pd.read_table(metadata_file, index_col=0)
else:
metadata = None
# filter out low abundance microbes and split table
res = split_tables(
microbes, metabolites,
metadata=metadata, training_column=training_column,
num_test=num_testing_examples,
min_samples=min_feature_count)
(train_microbes_df, test_microbes_df,
train_metabolites_df, test_metabolites_df) = res
params = []
sname = 'latent_dim_' + str(latent_dim) + \
'_input_prior_%.2f' % input_prior + \
'_output_prior_%.2f' % output_prior + \
'_beta1_%.2f' % beta1 + \
'_beta2_%.2f' % beta2
sname = os.path.join(summary_dir, sname)
if embeddings_file is None:
embeddings_file = sname + "_embedding.txt"
if ranks_file is None:
ranks_file = sname + "_ranks.txt"
if ordination_file is None:
ordination_file = sname + "_ordination.txt"
n, d1 = microbes.shape
n, d2 = metabolites.shape
train_microbes_coo = coo_matrix(train_microbes_df.values)
test_microbes_coo = coo_matrix(test_microbes_df.values)
if arm_the_gpu:
# pick out the first GPU
device_name='/device:GPU:0'
else:
device_name='/cpu:0'
config = tf.ConfigProto()
with tf.Graph().as_default(), tf.Session(config=config) as session:
model = MMvec(
latent_dim=latent_dim,
u_scale=input_prior, v_scale=output_prior,
learning_rate = learning_rate,
beta_1=beta1, beta_2=beta2,
device_name=device_name,
clipnorm=clipnorm, save_path=sname)
model(session,
train_microbes_coo, train_metabolites_df.values,
test_microbes_coo, test_metabolites_df.values)
loss, cv = model.fit(epoch=epochs, summary_interval=summary_interval,
checkpoint_interval=checkpoint_interval)
pc_ids = list(range(latent_dim))
vdim = model.V.shape[0]
V = np.hstack((np.zeros((vdim, 1)), model.V))
V = V.T
Vbias = np.hstack((np.zeros(1), model.Vbias.ravel()))
# Save to an embeddings file
Uparam = format_params(model.U, pc_ids, list(train_microbes_df.columns), 'microbe')
Vparam = format_params(V, pc_ids, list(train_metabolites_df.columns), 'metabolite')
df = pd.concat(
(
Uparam, Vparam,
format_params(model.Ubias, ['bias'], train_microbes_df.columns, 'microbe'),
format_params(Vbias, ['bias'], train_metabolites_df.columns, 'metabolite')
), axis=0)
df.to_csv(embeddings_file, sep='\t')
# Save to a ranks file
ranks = pd.DataFrame(model.ranks(), index=train_microbes_df.columns,
columns=train_metabolites_df.columns)
u, s, v = svds(ranks - ranks.mean(axis=0), k=latent_dim)
ranks = ranks.T
ranks.index.name = 'featureid'
ranks.to_csv(ranks_file, sep='\t')
# Save to an ordination file
s = s[::-1]
u = u[:, ::-1]
v = v[::-1, :]
microbe_embed = u @ np.diag(s)
metabolite_embed = v.T
pc_ids = ['PC%d' % i for i in range(microbe_embed.shape[1])]
features = pd.DataFrame(
microbe_embed, columns=pc_ids,
index=train_microbes_df.columns)
samples = pd.DataFrame(
metabolite_embed, columns=pc_ids,
index=train_metabolites_df.columns)
short_method_name = 'mmvec biplot'
long_method_name = 'Multiomics mmvec biplot'
eigvals = pd.Series(s, index=pc_ids)
proportion_explained = pd.Series(s**2 / np.sum(s**2), index=pc_ids)
biplot = OrdinationResults(
short_method_name, long_method_name, eigvals,
samples=samples, features=features,
proportion_explained=proportion_explained)
biplot.write(ordination_file)
if __name__ == '__main__':
mmvec()