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Equivolumetric protocol generates library sizes proportional to total microbial load in 16S amplicon sequencing

Repository with data and code for the analyses involved in the corresponding manuscript (https://doi.org/10.3389/fmicb.2021.638231).

• All Supplementary Materials .Rmd files use data/normalized/phyloseq.rds as primary input.
• Within each .Rmd file, you will need to set the variable REFIT = TRUE in order to fit the models and save corresponding (quite big) .rds files.
• Once the .rds files for the fitted models and cross-validations exist, you can just set REFIT = FALSE and knit the .Rmd files to produce beautiful .html reports.

All sequence data are deposited in NCBI BioProject PRJNA603167.

Dependencies:

R v. 3.6.3

brms v. 2.14.4

docstring v. 1.0.0

furrr v. 0.2.2

ggpubr v. 0.4.0

ggridges v. 0.5.3

latex2exp v. 0.4.0

modelr v. 0.1.8

patchwork v. 1.1.1

phyloseq v. 1.30.0

RColorBrewer v. 1.1.2

Rcpp v. 1.0.6

rlang v. 0.4.10

rms v. 6.1.0

tidybayes v. 2.3.1

tidyverse v. 1.3.0

caret v. 6.0.86

future v. 1.21.0

DescTools v. 0.99.39

rafalib v. 1.0.0

scales v. 1.1.1

plyr v. 1.8.6

ggrepel v. 0.9.1

knitr v. 1.31