NLM-HiLo

This content is based on our bioRxiv preprint titled "Multiplane HiLo microscopy with speckle illumination and non-local means denoising".

Background

HiLo microscopy is a variant of structured illumination microscopy. It makes use of an uniform-illumination image and a structured illumination image (with a grid or speckle pattern) to achieve widefield optical sectioning.

Implementation of basic speckle-based HiLo reconstruction and non-local means (NLM) denoising to reduce residual speckle noise in the processed image is provided here.

Instructions

• Set up environment:

MATLAB 2021a

Visual Studio 2019

Check here to see if your version of Visual C++ is supported in MATLAB.

• Run HiLo example

main_test.m

A single image $(571\times 571)$ with a search window width of $31\times 31$ should take ~9s to process.

Contact

Please reach out to sqzheng@bu.edu with any questions.