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Although the background channel is useful for the cell classification and registration steps, it is not always practical to double the data collected (and in the case of some fluorophores, a channel with only auto-fluorescence can be hard to obtain). It should be possible to supply one channel and:
Only register this one channel
Use 3D, rather than 4D input to the classification network (at the expense of poorer performance).
The text was updated successfully, but these errors were encountered:
Although the background channel is useful for the cell classification and registration steps, it is not always practical to double the data collected (and in the case of some fluorophores, a channel with only auto-fluorescence can be hard to obtain). It should be possible to supply one channel and:
The text was updated successfully, but these errors were encountered: