BioBytes PairSync: A Bioinformatics Tool for Basic Information Retrieval and Pairwise Sequence Alignment

Download Installer!

Click on "biobytes_pairsync_setup.exe" at the releases page.

Demo

This GUI app allows users to input biological sequences, either manually or by loading from a file. It supports DNA, RNA, and Protein sequences. Users can retrieve various information about the input sequence, such as nucleotide or amino acid frequency, GC content, reverse complement, transcription, translation, amino acid molecular weight, isoelectric point, and open reading frames.

Users can save the output information to a text file and clear the output section for a clean slate. Additionally, loading sequences from FASTA or text files is supported, making it convenient for users to work with existing sequence data.

Enjoy exploring the functionalities provided by this GUI app for basic sequence analysis!

Usage

Basic Information

• Please select the Biomolecule Type of your data (DNA, RNA, or Protein).

• Add a Label (Optional). It would add "No Sample Label Added" if no entry added

• Add the sequence Input Box. Make sure it is in correct format and without whitespaces. NCBI URL is also accepted under the Nucleotide Database.

• You can Clear the output or Save it as .txt file

Dot Plot Alignment

• Input your first and second sequence at Sequence 1 and Sequence 2 box respectively. Additionally, it is optional to add a Label

• Take note that whitespaces and totally unequal lengths prompts error and not allowed for this alignment

It is highly recommended to add Label as it would appear in the plot

• The Plot Button allows to generate the Dot Plot with associated information of match & mismatch bases/amino acids.

• The output can be saved by clicking the Save Button as .png file

Local and Global Alignment

• Both Local and Global Alignment had the same User Interface Layout but they differ in the algorithm used since they have different purpose.

• Input your first and second sequence at Sequence 1 and Sequence 2 box respectively. Additionally, it is optional to add a Label

• Make sure no whitespaces included on the sequence and they are compatible for pairwise alignment (sequence length may be different but not applicable for pair of sequences which has large difference in length)

• You can Clear the output or Save it as .txt file

Thank you for using my developed tool 🚢

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Developer: Bryan Castillo

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