0.4.0

Changelog

New features

Data compatibility

• Add support for custom MINFLUX sequences.
• Add initial support for single-molecule tracking datasets.
• Update the pyMINFLUX Reader ParaView plugin to read .pmx version 2.0 files.
• Add macOS M1 build of the pyMINFLUX application.

Analysis and filtering tools

• Merge Trace Length Viewer tool into the Analyzer.
• Implement filtering by trace length into the Analyzer.
• Add per-trace tracking stats to the Trace Stats Viewer (tracking datasets only).
• Add Histogram Plotter tool.
• Implement Set range context-menu action in the Time Inspector.

UI

• Switch to green-magenta color scheme for fluorophore IDs.
• Add a scale bar to the main data viewer (for XYZ localisations).
• Display line connecting subsequent localizations within a trace (tracking datasets only).

Performance improvements

• Dramatically improve the rendering of color-coded localizations.
• Speed up dataframe-based operations by upgrading to Pandas 2.2.x with PyArrow backend.

Fixes

• Fix default axis ranges not being applied to the Analyzer plots when loading new data.
• Fix (incorrectly) forcing the aspect ratio of the Data Plotter for XYZ localizations.

0.3.0

Changelog

New features

• Add native pyMINFLUX file format .pmx.
• FRC Analysis now runs in parallel over all CPU cores.
• Add new Trace Statistics Viewer.
• Add new Trace Length Viewer.
• Add fluorophore ID reassignment within traces by majority vote.
• Allow opening files by drag-and-drop onto the Pipeline Toolbar (left panel).
• Add all analysis tools to the main window's Analysis menu.
• Add companion ParaView reader plug-in.
• Remove 3D Plotter (in favor of using ParaView and the ParaView reader plug-in).

0.2.0

Changelog

New features

• Calculate additional per-trace statistics and allow exporting all stats to csv file.
• Add Fourier Ring Correlation tool to follow resolution progression.
• Allow complete access to the API for command-line/notebook processing: https://pyminflux.ethz.ch/api/pyminflux.
  • Example notebook
• Add menu action to check for application updates.

Fixed

• Fix issue with the fluorophore drop-down menu not being refreshed after global reset button is pressed.

0.1.3

Changelog

New features

• Implement z-scaling factor to compensate for refractive index mismatch (issue #1).
• Add context menu actions to export any plot as a .png image and at a user-defined DPI (issue #3).
• Add global reset button to the main-window wizard and remove the one from the Analyzer.
• Add quick help for all entries in the Options dialog.
• Add links to code repository, issues page, and mailing list to the Help menu.
• Add a few minor UI tweaks.

Fixed

• Fix issue with applying a filter in the Time Inspector not updating the Analyzer plots.
• Fix issue with measurement lines being allowed when the plotted data was not spatial (x, y), and the reported distance would be in nm.
• Fix inconsistency in the way fluorophore IDs and colors were assigned by the various methods used.

0.1.2

Changelog

• Fix issue with exporting to .npy only working when the Active Color selector was on All.

0.1.1

This is the first official release of pyMINFLUX.