TEMMitoMacro is a pair of open-source ImageJ and Fiji macros that, together, allow for the semi-automated batch analysis of mitchondrial morphology in TEM images.
Parameters analysed are based on previously implemented measures in the literature: area, area^2, form factor, aspect ratio, perimeter, circularity, Feret’s diameter and roundness (ie as per Merrill, Flippo & Strack, 2017).
TEMMitoMacro consistes of 2 Macro files: (1) ROIBatchGen, which allows for the selection of ROIs to be used by the second macro, (2)EM-MitoMorphMacro, which automatically analyses mitochondrial morphology in the generated ROI files and generates population statistics. This macro pair was developed in collaboration with Erin Buchanan from the O'Ryan Lab at the University of Cape Town.
TEMMitoMacro is available for download The .ijm files can be opened and run directly in Fiji/ImageJ.