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blogdump.xml
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<?xml version="1.0" encoding="UTF-8"?>
<posts to="2010-09-23T11:36:17+01:00" from="2008-10-09T11:22:07+01:00"><post><id>75</id><rid>75</rid><title>Lab SAXS Template</title><section>Templates</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on the SAXSess instrument in the Edler group at the University of Bath. The samples were run in the capillary cell at a temperature of 25 C.
[table]
[row]Sample[col]Exposure time (min)[col]Run #[col]Data[/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Blog]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[/table]]]></content><html><![CDATA[The following samples were run as described on the SAXSess instrument in the Edler group at the University of Bath. The samples were run in the capillary cell at a temperature of 25 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Exposure time (min)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Blog]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr></table><br/>
]]></html><datestamp>2008-10-09T11:22:07+01:00</datestamp><timestamp>2008-10-09T11:22:07+01:00</timestamp><blog>5</blog><key>b5a2b3c856a5b282ebaf1c6677c98ff5</key><links><uri>http://biolab.isis.rl.ac.uk/uri/20</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/75/Lab_SAXS_Template.png</format></formats><comments/></post><post><id>81</id><rid>81</rid><title>GFP solution 5 mg/ml</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A 5mg/mL solution of GFP in [blog]76[/blog] prepared in [blog]79[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
A 5mg/mL solution of GFP in <a href="camerons_labblog/76/Sortase_Buffer.html">Sortase Buffer</a> prepared in <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;<a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T11:32:10+01:00</datestamp><timestamp>2008-10-09T11:32:10+01:00</timestamp><blog>5</blog><key>03b54a3da0d896d20d6c1388c15d954a</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/25</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/81/GFP_solution_5_mgml.png</format></formats><comments/></post><post><id>82</id><rid>82</rid><title>GFP solution 2 mg/ml</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A 2mg/mL solution of GFP in Sortase Buffer prepared in [blog]79[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
A 2mg/mL solution of GFP in Sortase Buffer prepared in <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;
]]></html><datestamp>2008-10-09T11:33:25+01:00</datestamp><timestamp>2008-10-09T11:33:25+01:00</timestamp><blog>5</blog><key>3f3fac49af43fadb082ddb4b0253bd27</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/26</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/82/GFP_solution_2_mgml.png</format></formats><comments/></post><post><id>83</id><rid>83</rid><title>GFP solution 1 mg/ml</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A 2mg/mL solution of GFP in Sortase Buffer prepared in [blog]79[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
A 2mg/mL solution of GFP in Sortase Buffer prepared in <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T12:03:10+01:00</datestamp><timestamp>2008-10-09T12:03:10+01:00</timestamp><blog>5</blog><key>f4b3e95420fd15c7d322aba34d053fc9</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/27</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/83/GFP_solution_1_mgml.png</format></formats><comments/></post><post><id>79</id><rid>84</rid><title>Preparation of GFP solutions</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Carried out by Maria Gomis-Gomis.
9.7 mg of [blog]77[/blog] was resuspended in 970 uL of [blog]76[/blog] to yield [blog]78[/blog].
This solution was then diluted with [blog]76[/blog] to yield:
[blog]81[/blog]
[blog]82[/blog]
[blog]83[/blog]]]></content><html><![CDATA[Carried out by Maria Gomis-Gomis. <br style="clear:left;"/><br style="clear:left;"/>9.7 mg of <a href="camerons_labblog/77/Freeze_dried_GFP.html">Freeze dried GFP</a> was resuspended in 970 uL of <a href="camerons_labblog/76/Sortase_Buffer.html">Sortase Buffer</a> to yield <a href="camerons_labblog/78/GFP_solution_10_mgmL.html">GFP solution 10 mg/mL</a>.<br style="clear:left;"/><br style="clear:left;"/>This solution was then diluted with <a href="camerons_labblog/76/Sortase_Buffer.html">Sortase Buffer</a> to yield:<br style="clear:left;"/><br style="clear:left;"/><a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a><br style="clear:left;"/><a href="camerons_labblog/82/GFP_solution_2_mgml.html">GFP solution 2 mg/ml</a><br style="clear:left;"/><a href="camerons_labblog/83/GFP_solution_1_mgml.html">GFP solution 1 mg/ml</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a>;<a href="camerons_labblog/82/GFP_solution_2_mgml.html">GFP solution 2 mg/ml</a>;<a href="camerons_labblog/83/GFP_solution_1_mgml.html">GFP solution 1 mg/ml</a>;<a href="camerons_labblog/78/GFP_solution_10_mgmL.html">GFP solution 10 mg/mL</a>;
]]></html><datestamp>2008-10-09T11:31:01+01:00</datestamp><timestamp>2008-10-09T12:04:55+01:00</timestamp><blog>5</blog><key>47825913e13aca36e785087a55032097</key><links><uri>http://biolab.isis.rl.ac.uk/uri/24</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/79/Preparation_of_GFP_solutions.png</format></formats><comments/></post><post><id>86</id><rid>86</rid><title>Centrifuged 10 mg/mL GFP</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The centrifuged product of [blog]85[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
The centrifuged product of <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:06:36+01:00</datestamp><timestamp>2008-10-09T16:06:36+01:00</timestamp><blog>5</blog><key>a0dcc82ec201f32d805c81ddb4744fba</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/29</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/86/Centrifuged_10_mgmL_GFP.png</format></formats><comments/></post><post><id>87</id><rid>87</rid><title>Centrifuged 2 mg/mL GFP</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[ Spun solution of GFP at 2 mg/mL from [blog]85[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
Spun solution of GFP at 2 mg/mL from <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:08:11+01:00</datestamp><timestamp>2008-10-09T16:08:11+01:00</timestamp><blog>5</blog><key>7e46e48632324368bbcf9e798e04c9c8</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/2a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/87/Centrifuged_2_mgmL_GFP.png</format></formats><comments/></post><post><id>88</id><rid>88</rid><title>Centrifuged 5 mg/mL GFP</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Spun solution of GFP at 5 mg/mL from [blog]85[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
Spun solution of GFP at 5 mg/mL from <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html">Centrifugation of 10 mg/mL GFP solution</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:09:03+01:00</datestamp><timestamp>2008-10-09T16:09:03+01:00</timestamp><blog>5</blog><key>eba9f8e012fb65668fcb5a5c0aa7f658</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/2b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/88/Centrifuged_5_mgmL_GFP.png</format></formats><comments/></post><post><id>110</id><rid>114</rid><title>SAXS Run 02075</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T18:16:04+01:00</datestamp><timestamp>2008-10-09T18:21:56+01:00</timestamp><blog>5</blog><key>5226880bb4b88fa8caf705aa0ef1f030</key><metadata><data_type>SAXS_Lab</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/36</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/110/SAXS_Run_02075.png</format></formats><comments/></post><post><id>108</id><rid>115</rid><title>SAXS Run 02074</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T18:14:04+01:00</datestamp><timestamp>2008-10-09T18:22:15+01:00</timestamp><blog>5</blog><key>03f2e16e6153cd78718819a4d4523a46</key><metadata><data_type>SAXS_Lab</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/34</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/108/SAXS_Run_02074.png</format></formats><comments/></post><post><id>97</id><rid>116</rid><title>SAXS Run 02073</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:16:15+01:00</datestamp><timestamp>2008-10-09T18:22:29+01:00</timestamp><blog>5</blog><key>453bd92341a5e66790fed62e7a739f1a</key><metadata><data_type>SAXS_Lab</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/33</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/97/SAXS_Run_02073.png</format></formats><comments/></post><post><id>96</id><rid>117</rid><title>SAXS Run 02072</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:16:04+01:00</datestamp><timestamp>2008-10-09T18:22:48+01:00</timestamp><blog>5</blog><key>93138f5668a95d90a7580769cc3193a2</key><metadata><data_type>SAXS_Lab</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/2.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/32</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/96/SAXS_Run_02072.png</format></formats><comments/></post><post><id>95</id><rid>118</rid><title>SAXS Run 02071</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:15:47+01:00</datestamp><timestamp>2008-10-09T18:23:03+01:00</timestamp><blog>5</blog><key>da3545253c5f94d524e931ded2fa12fc</key><metadata><data_type>SAXS_Lab</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/4.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/31</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/95/SAXS_Run_02071.png</format></formats><comments/></post><post><id>94</id><rid>119</rid><title>SAXS Run 02070</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:15:35+01:00</datestamp><timestamp>2008-10-09T18:23:18+01:00</timestamp><blog>5</blog><key>da627ffbde618aaee605f54973001401</key><metadata><data_type>SAXS_Lab</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/6.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/30</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/94/SAXS_Run_02070.png</format></formats><comments/></post><post><id>93</id><rid>120</rid><title>SAXS Run 02069</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The processing was aborted because the image plate was misplaced on the reader.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The processing was aborted because the image plate was misplaced on the reader.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:15:23+01:00</datestamp><timestamp>2008-10-09T18:23:32+01:00</timestamp><blog>5</blog><key>0b17c1565dd38cb842a102bdbcda63ff</key><metadata><data_type>SAXS_Lab</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/2f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/93/SAXS_Run_02069.png</format></formats><comments/></post><post><id>92</id><rid>121</rid><title>SAXS Run 02068</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:15:11+01:00</datestamp><timestamp>2008-10-09T18:23:47+01:00</timestamp><blog>5</blog><key>bfac81fb3e17a826ef3316bfd8886095</key><metadata><data_type>SAXS_Lab</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/8.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/2e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/92/SAXS_Run_02068.png</format></formats><comments/></post><post><id>91</id><rid>122</rid><title>SAXS Run 02067</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Lab<br />
The raw image (tiff) was collected from the image plate and processed using SAXS quant software (mask file). The X-scale was normalised by finding the beam centre (Q=0) and resetting the scale to nm<sup>-1</sup>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T16:14:13+01:00</datestamp><timestamp>2008-10-09T18:24:49+01:00</timestamp><blog>5</blog><key>e0499d02179261a070388613e744bb55</key><metadata><data_type>SAXS_Lab</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/10.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/2d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/91/SAXS_Run_02067.png</format></formats><comments/></post><post><id>123</id><rid>128</rid><title>GFP SAXS data reduction</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The pdh files generated in [blog]109[/blog] were all binned from Q = 0.08 - 20 nm<sup>-1</sup> to generate the associated bin files. The background [blog]91[/blog] was subtracted from all 30 minute sample runs to generate the associated sub files.
The rebinned data from [blog]91[/blog] and [blog]110[/blog] were summed to give 02075.add. This was used as the background for the 60 minute runs ([blog]95[/blog] and [blog]108[/blog]) as well as for the sum of two runs of [blog]81[/blog] designated 02070.add
All data files are provided at:
http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10
The background subtracted data were re-binned to generate the files designated #####b.bin. Binning range was selected to run from 0.25 nm<sup>-1</sup> to where the scattering was deemed to reach noise levels (can be seen from the data files). The binned files were de-smeared using the provided Lake software with default parameters. These generated the associated des files.
All re-binned and de-smeared data files are provided at:
http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10
The data from the 1mg/mL solution [blog]94[/blog] was not desmeared as after background subtraction it had very little signal.]]></content><html><![CDATA[The pdh files generated in <a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a> were all binned from Q = 0.08 - 20 nm<sup>-1</sup> to generate the associated bin files. The background <a href="camerons_labblog/91/SAXS_Run_02067.html">SAXS Run 02067</a> was subtracted from all 30 minute sample runs to generate the associated sub files. <br style="clear:left;"/><br style="clear:left;"/>The rebinned data from <a href="camerons_labblog/91/SAXS_Run_02067.html">SAXS Run 02067</a> and <a href="camerons_labblog/110/SAXS_Run_02075.html">SAXS Run 02075</a> were summed to give 02075.add. This was used as the background for the 60 minute runs (<a href="camerons_labblog/95/SAXS_Run_02071.html">SAXS Run 02071</a> and <a href="camerons_labblog/108/SAXS_Run_02074.html">SAXS Run 02074</a>) as well as for the sum of two runs of <a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a> designated 02070.add<br style="clear:left;"/><br style="clear:left;"/>All data files are provided at:<br style="clear:left;"/> <br style="clear:left;"/><a href="http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10">http://research.google.com/datasets/id/grd20081000149/files#snapshot=2008-10-10</a><br style="clear:left;"/><br style="clear:left;"/>The background subtracted data were re-binned to generate the files designated #####b.bin. Binning range was selected to run from 0.25 nm<sup>-1</sup> to where the scattering was deemed to reach noise levels (can be seen from the data files). The binned files were de-smeared using the provided Lake software with default parameters. These generated the associated des files. <br style="clear:left;"/><br style="clear:left;"/>All re-binned and de-smeared data files are provided at:<br style="clear:left;"/><br style="clear:left;"/><a href="http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10">http://research.google.com/datasets/id/grd20081000177/files#snapshot=2008-10-10</a><br style="clear:left;"/><br style="clear:left;"/>The data from the 1mg/mL solution <a href="camerons_labblog/94/SAXS_Run_02070.html">SAXS Run 02070</a> was not desmeared as after background subtraction it had very little signal.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.html">Maria's analysis of the GFP SAXS data</a>;
]]></html><datestamp>2008-10-09T19:11:32+01:00</datestamp><timestamp>2008-10-10T14:57:10+01:00</timestamp><blog>5</blog><key>f058512dd6813c498098b957e07ea341</key><links><uri>http://biolab.isis.rl.ac.uk/uri/37</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/123/GFP_SAXS_data_reduction.png</format></formats><comments/></post><post><id>85</id><rid>129</rid><title>Centrifugation of 10 mg/mL GFP solution</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Aggregation was evident in the 10 mg/mL GFP solution so it was spun (10 minutes, 11krpm in Eppendorf benchtop centrifuge) to give [blog]86[/blog]
From this solution fresh solutions of [blog]88[/blog] and [blog]87[/blog]]]></content><html><![CDATA[Aggregation was evident in the 10 mg/mL GFP solution so it was spun (10 minutes, 11krpm in Eppendorf benchtop centrifuge) to give <a href="camerons_labblog/86/Centrifuged_10_mgmL_GFP.html">Centrifuged 10 mg/mL GFP</a> <br style="clear:left;"/><br style="clear:left;"/>From this solution fresh solutions of <a href="camerons_labblog/88/Centrifuged_5_mgmL_GFP.html">Centrifuged 5 mg/mL GFP</a> and <a href="camerons_labblog/87/Centrifuged_2_mgmL_GFP.html">Centrifuged 2 mg/mL GFP</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/86/Centrifuged_10_mgmL_GFP.html">Centrifuged 10 mg/mL GFP</a>;<a href="camerons_labblog/87/Centrifuged_2_mgmL_GFP.html">Centrifuged 2 mg/mL GFP</a>;<a href="camerons_labblog/88/Centrifuged_5_mgmL_GFP.html">Centrifuged 5 mg/mL GFP</a>;
]]></html><datestamp>2008-10-09T16:06:16+01:00</datestamp><timestamp>2008-10-13T13:38:51+01:00</timestamp><blog>5</blog><key>093a2979502fbd4116f64a554b0127b5</key><links><uri>http://biolab.isis.rl.ac.uk/uri/28</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/85/Centrifugation_of_10_mgmL_GFP_solution.png</format></formats><comments/></post><post><id>131</id><rid>131</rid><title>GFP (10 mg/mL) in D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A 10 mg/mL solution of GFP in D2O buffer prepared in [blog]130[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
A 10 mg/mL solution of GFP in D2O buffer prepared in <a href="http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a><br/><b>This Post is Linked By:</b> <a href="http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/11913/Comparison_of_SANS2d_and_LOQ_GFP_data.html">Comparison of SANS2d and LOQ GFP data</a>;
]]></html><datestamp>2008-10-16T12:15:41+01:00</datestamp><timestamp>2008-10-16T12:15:41+01:00</timestamp><blog>5</blog><key>c28ba56169ef8c61b8cc7618ee879c21</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/39</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.png</format></formats><comments/></post><post><id>132</id><rid>132</rid><title>GFP 5 mg/mL in D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A 5 mg/mL solution of GFP in D2O phophate buffer prepared in [blog]130[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
A 5 mg/mL solution of GFP in D2O phophate buffer prepared in <a href="camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a>;<a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-16T12:51:18+01:00</datestamp><timestamp>2008-10-16T12:51:18+01:00</timestamp><blog>5</blog><key>ba50ac4c30f1e4327942358befc3d09b</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/3a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.png</format></formats><comments/></post><post><id>133</id><rid>133</rid><title>GFP 2 mg/mL in D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[An approx 2mg/mL solution of GFP in D2O phosphate buffer prepared in ]]></content><html><![CDATA[<b>Material:</b> Solution<br />
An approx 2mg/mL solution of GFP in D2O phosphate buffer prepared in <br/><b>This Post is Linked By:</b> <a href="camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a>;<a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-16T12:51:55+01:00</datestamp><timestamp>2008-10-16T12:51:55+01:00</timestamp><blog>5</blog><key>4f8c138e5699553c08d251674adfa964</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/3b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.png</format></formats><comments/></post><post><id>130</id><rid>134</rid><title>Preparation of GFP Samples for a quickie SANS experiment on LOQ</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[blog]77[/blog] (10.5 mg) was disolved in 1.00 mL of 20 mM phosphate buffer in D2O. After addition the tube and contents weighed 1.1010g. The solution was then spun for 10' (20k x g) (to give [blog]131[/blog].
The solution was then diluted (300 uL + 300 uL buffer) to give [blog]132[/blog] and then this solution was diluted further (100 uL + 150 uL of buffer) to give [blog]133[/blog].]]></content><html><![CDATA[<a href="camerons_labblog/77/Freeze_dried_GFP.html">Freeze dried GFP</a> (10.5 mg) was disolved in 1.00 mL of 20 mM phosphate buffer in D2O. After addition the tube and contents weighed 1.1010g. The solution was then spun for 10' (20k x g) (to give <a href="camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html">GFP (10 mg/mL) in D2O buffer</a>.<br style="clear:left;"/><br style="clear:left;"/>The solution was then diluted (300 uL + 300 uL buffer) to give <a href="camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html">GFP 5 mg/mL in D2O buffer</a> and then this solution was diluted further (100 uL + 150 uL of buffer) to give <a href="camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.html">GFP 2 mg/mL in D2O buffer</a>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html">GFP (10 mg/mL) in D2O buffer</a>;<a href="camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html">GFP 5 mg/mL in D2O buffer</a>;
]]></html><datestamp>2008-10-16T12:15:05+01:00</datestamp><timestamp>2008-10-16T12:55:24+01:00</timestamp><blog>5</blog><key>8d73b9766fc7ba43334947ede0adbc56</key><links><uri>http://biolab.isis.rl.ac.uk/uri/38</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.png</format></formats><comments/></post><post><id>135</id><rid>135</rid><title>Lysozyme</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Fisher lysozyme powder]]></content><html><![CDATA[<b>Material:</b> Powder<br />
Fisher lysozyme powder<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html">Preparation of lysozyme solutions for quickie SANS experiment</a>;
]]></html><datestamp>2008-10-16T15:13:20+01:00</datestamp><timestamp>2008-10-16T15:13:20+01:00</timestamp><blog>5</blog><key>494b31358edd5c631b5e38876000a1e2</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/3c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/135/Lysozyme.png</format></formats><comments/></post><post><id>137</id><rid>137</rid><title>Lysozyme 10 mg/mL in D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[10 mg/mL solution of lysozyme in D2O buffer preapred in[blog]136[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
10 mg/mL solution of lysozyme in D2O buffer preapred in<a href="camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html">Preparation of lysozyme solutions for quickie SANS experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html">Preparation of lysozyme solutions for quickie SANS experiment</a>;<a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-16T15:15:44+01:00</datestamp><timestamp>2008-10-16T15:15:44+01:00</timestamp><blog>5</blog><key>f6aeee02449034bd4ed2d71f187f94ba</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/3e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.png</format></formats><comments/></post><post><id>138</id><rid>138</rid><title>Lysozyme 2mg/mL</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[2 mg/mL solution of lysozyme prepared in [blog]136[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
2 mg/mL solution of lysozyme prepared in <a href="camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html">Preparation of lysozyme solutions for quickie SANS experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html">Preparation of lysozyme solutions for quickie SANS experiment</a>;<a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-16T15:16:25+01:00</datestamp><timestamp>2008-10-16T15:16:25+01:00</timestamp><blog>5</blog><key>7b680eecebc1d358b07a5b224ea3c577</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/3f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/138/Lysozyme_2mgmL.png</format></formats><comments/></post><post><id>136</id><rid>139</rid><title>Preparation of lysozyme solutions for quickie SANS experiment</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[blog]135[/blog] (17.6 mg) was dissolved in D2O phophate buffer (880 uL) to give a 20 mg/mL solution. The solution was diluted (400uL + 400 uL buffer) to give [blog]137[/blog]. This solution was diluted (100 uL + 400 uL of buffer) to give [blog]138[/blog].]]></content><html><![CDATA[<a href="camerons_labblog/135/Lysozyme.html">Lysozyme</a> (17.6 mg) was dissolved in D2O phophate buffer (880 uL) to give a 20 mg/mL solution. The solution was diluted (400uL + 400 uL buffer) to give <a href="camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html">Lysozyme 10 mg/mL in D2O buffer</a>. This solution was diluted (100 uL + 400 uL of buffer) to give <a href="camerons_labblog/138/Lysozyme_2mgmL.html">Lysozyme 2mg/mL</a>.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html">Lysozyme 10 mg/mL in D2O buffer</a>;<a href="camerons_labblog/138/Lysozyme_2mgmL.html">Lysozyme 2mg/mL</a>;
]]></html><datestamp>2008-10-16T15:15:30+01:00</datestamp><timestamp>2008-10-16T15:17:23+01:00</timestamp><blog>5</blog><key>65982a5385ead0c16f723448ee425099</key><links><uri>http://biolab.isis.rl.ac.uk/uri/3d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/136/Preparation_of_lysozyme_solutions_for_quickie_SANS_experiment.png</format></formats><comments/></post><post><id>141</id><rid>141</rid><title>20 mM Phosphate buffer in D2O</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Made by Luke]]></content><html><![CDATA[<b>Material:</b> Solution<br />
Made by Luke<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-16T15:52:45+01:00</datestamp><timestamp>2008-10-16T15:52:45+01:00</timestamp><blog>5</blog><key>c92a79433ae5ced830a7003d2afae744</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/41</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.png</format></formats><comments/></post><post><id>171</id><rid>171</rid><title>UV-Vis spectroscopy of GFP and Lysozyme samples</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Samples were run in 1mm banjo cells that were used for the SANS experiment. The samples were held in place by white-tac. The JASCO UV-Vis spectrophotometer was zeroed with no samples and then the baseline checked and re-zeroed with two buffer samples. Spectra were recorded with buffer in the reference position.
[table]
[row]Sample[col]Data[/row]
[row][blog]131[/blog][col][blog]144[/blog][/row]
[row][blog]132[/blog][col][blog]147[/blog][/row]
[row][blog]133[/blog][col][blog]150[/blog][/row]
[row][blog]137[/blog][col][blog]153[/blog][/row]
[row][blog]138[/blog][col][blog]156[/blog][/row]
[/table]
]]></content><html><![CDATA[<b>Procedure:</b> UV-Vis<br />
Samples were run in 1mm banjo cells that were used for the SANS experiment. The samples were held in place by white-tac. The JASCO UV-Vis spectrophotometer was zeroed with no samples and then the baseline checked and re-zeroed with two buffer samples. Spectra were recorded with buffer in the reference position.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html">GFP (10 mg/mL) in D2O buffer</a></td><td class="table_st"><a href="camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.html">UV-Vis spectrum of 10mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html">GFP 5 mg/mL in D2O buffer</a></td><td class="table_st"><a href="camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.html">UV-Vis spectrum of 5mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.html">GFP 2 mg/mL in D2O buffer</a></td><td class="table_st"><a href="camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.html">UV-Vis spectrum of 2mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html">Lysozyme 10 mg/mL in D2O buffer</a></td><td class="table_st"><a href="camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.html">UV-Vis spectrum of 10mg/mL Lysozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/138/Lysozyme_2mgmL.html">Lysozyme 2mg/mL</a></td><td class="table_st"><a href="camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.html">UV-Vis spectrum of 2 mg/mL Lysozyme</a></td></tr></table><br style="clear:left;"/><br/>
]]></html><datestamp>2008-10-20T11:32:11+01:00</datestamp><timestamp>2008-10-20T11:32:11+01:00</timestamp><blog>5</blog><key>c759aa20b21936714c6ffa55b0a80402</key><metadata><procedure>UV-Vis</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/48</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.png</format></formats><comments/></post><post><id>90</id><rid>173</rid><title>LOQ SANS data template</title><section>Templates</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.
[[Section>Data]]
[[Data_type>SANS_LOQ]]]]></content><html><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br style="clear:left;"/><br style="clear:left;"/>[[Section>Data]]<br style="clear:left;"/>[[Data_type>SANS_LOQ]]<br/>
]]></html><datestamp>2008-10-09T16:12:42+01:00</datestamp><timestamp>2008-10-20T11:39:49+01:00</timestamp><blog>5</blog><key>3cb28ca8e67dcbca93ec449dff3c70d7</key><links><uri>http://biolab.isis.rl.ac.uk/uri/2c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/90/LOQ_SANS_data_template.png</format></formats><comments/></post><post><id>174</id><rid>177</rid><title>44700 - 10mg/mL GFP on LOQ</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.]]></content><html><![CDATA[<b>Data Type:</b> SANS_LOQ<br />
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html">Initial analysis of SANS data on GFP</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-20T11:40:22+01:00</datestamp><timestamp>2008-10-20T11:41:14+01:00</timestamp><blog>5</blog><key>636ca1234b3d40afab26dd24f5a9d00a</key><metadata><data_type>SANS_LOQ</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/32.xml</data><data>http://biolab.isis.rl.ac.uk/data/34.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/49</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.png</format></formats><comments/></post><post><id>178</id><rid>181</rid><title>44701 - 5mg/mL GFP on LOQ</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.]]></content><html><![CDATA[<b>Data Type:</b> SANS_LOQ<br />
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html">Initial analysis of SANS data on GFP</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-20T12:02:57+01:00</datestamp><timestamp>2008-10-20T12:03:44+01:00</timestamp><blog>5</blog><key>dbdad992d82be85e0141eab767f16f1e</key><metadata><data_type>SANS_LOQ</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/36.xml</data><data>http://biolab.isis.rl.ac.uk/data/38.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/4a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.png</format></formats><comments/></post><post><id>182</id><rid>185</rid><title>44702 - 2mg/mL GFP on LOQ</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.]]></content><html><![CDATA[<b>Data Type:</b> SANS_LOQ<br />
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html">Initial analysis of SANS data on GFP</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-20T12:03:58+01:00</datestamp><timestamp>2008-10-20T12:04:36+01:00</timestamp><blog>5</blog><key>98f3e53a69da806ea061880400784168</key><metadata><data_type>SANS_LOQ</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/40.xml</data><data>http://biolab.isis.rl.ac.uk/data/42.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/4b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.png</format></formats><comments/></post><post><id>186</id><rid>189</rid><title>44703 - 10mg/mL Lysozyme on LOQ</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.]]></content><html><![CDATA[<b>Data Type:</b> SANS_LOQ<br />
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br/><b>This Post is Linked By:</b> <a href="http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html">Initial analysis of SANS data on GFP</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/11921/Comparison_of_SANS2d_and_LOQ_Lysozyme_data.html">Comparison of SANS2d and LOQ Lysozyme data</a>;
]]></html><datestamp>2008-10-20T12:04:54+01:00</datestamp><timestamp>2008-10-20T12:05:30+01:00</timestamp><blog>5</blog><key>31a2c7d9fc3e6742ce67131d39e0fb6d</key><metadata><data_type>SANS_LOQ</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/44.xml</data><data>http://biolab.isis.rl.ac.uk/data/46.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/4c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.png</format></formats><comments/></post><post><id>190</id><rid>193</rid><title>44704 - 2mg/mL Lysozyme on LOQ</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.]]></content><html><![CDATA[<b>Data Type:</b> SANS_LOQ<br />
The raw image files were corrected and processed using Collete and using a buffer background. SASXML and LOQ Q files are included.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html">Initial analysis of SANS data on GFP</a>;<a href="camerons_labblog/142/SANS_on_GFP_concentration_series.html">SANS on GFP concentration series</a>;
]]></html><datestamp>2008-10-20T12:05:54+01:00</datestamp><timestamp>2008-10-20T12:06:41+01:00</timestamp><blog>5</blog><key>ca76931b8e905da561905fa5d06c4a7a</key><metadata><data_type>SANS_LOQ</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/48.xml</data><data>http://biolab.isis.rl.ac.uk/data/50.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/4d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.png</format></formats><comments/></post><post><id>195</id><rid>197</rid><title>Initial analysis of SANS data on GFP</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The data from the following files was loaded into Excel and graphed. Data from [blog]174[/blog], [blog]178[/blog], and [blog]186[/blog] was fairly good over the range of Q=0.01 - 0.1. Data from [blog]182[/blog] was ok and [blog]190[/blog] wasn't really useable.
Guiner plots gave an Rg of ~24 A for GFP and the experimental data was well fitted by that generated from the pdb file 1gfl ([url]http://www.pdb.org/pdb/explore/explore.do?structureId=1GFL[/url]) which is of a dimer. The Rg is consistent with a dimer. Trying to fit the data to that predicted from the monomer (prepared by deleting one chain from 1gfl) gave a very poor fit.
The lysozyme data is not fit well by data predicted from 2vb1 ([url]http://www.pdb.org/pdb/explore.do?structureId=2VB1[/url])]]></content><html><![CDATA[The data from the following files was loaded into Excel and graphed. Data from <a href="camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.html">44700 - 10mg/mL GFP on LOQ</a>, <a href="camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.html">44701 - 5mg/mL GFP on LOQ</a>, and <a href="camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.html">44703 - 10mg/mL Lysozyme on LOQ</a> was fairly good over the range of Q=0.01 - 0.1. Data from <a href="camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.html">44702 - 2mg/mL GFP on LOQ</a> was ok and <a href="camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.html">44704 - 2mg/mL Lysozyme on LOQ</a> wasn't really useable.<br style="clear:left;"/><br style="clear:left;"/>Guiner plots gave an Rg of ~24 A for GFP and the experimental data was well fitted by that generated from the pdb file 1gfl (<a href="http://www.pdb.org/pdb/explore/explore.do?structureId=1GFL" class="ng_url">http://www.pdb.org/pdb/explore/explore.do?structureId=1GFL</a>) which is of a dimer. The Rg is consistent with a dimer. Trying to fit the data to that predicted from the monomer (prepared by deleting one chain from 1gfl) gave a very poor fit.<br style="clear:left;"/><br style="clear:left;"/>The lysozyme data is not fit well by data predicted from 2vb1 (<a href="http://www.pdb.org/pdb/explore.do?structureId=2VB1" class="ng_url">http://www.pdb.org/pdb/explore.do?structureId=2VB1</a>)<br/>
]]></html><datestamp>2008-10-20T14:06:35+01:00</datestamp><timestamp>2008-10-20T14:07:15+01:00</timestamp><blog>5</blog><key>237204accab398d73eaeb9465c41bf84</key><attached_data><data>http://biolab.isis.rl.ac.uk/data/52.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/4e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/195/Initial_analysis_of_SANS_data_on_GFP.png</format></formats><comments/></post><post><id>156</id><rid>199</rid><title>UV-Vis spectrum of 2 mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[jws, jcamp, txt]]></content><html><![CDATA[<b>Data Type:</b> UV-VIS<br />
jws, jcamp, txt<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;
]]></html><datestamp>2008-10-17T12:18:30+01:00</datestamp><timestamp>2008-10-20T16:03:03+01:00</timestamp><blog>5</blog><key>af8d31033d76a2b1398cc724c5091f1d</key><metadata><data_type>UV-VIS</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/20.xml</data><data>http://biolab.isis.rl.ac.uk/data/22.xml</data><data>http://biolab.isis.rl.ac.uk/data/54.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/47</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/156/UVVis_spectrum_of_2_mgmL_Lysozyme.png</format></formats><comments/></post><post><id>153</id><rid>201</rid><title>UV-Vis spectrum of 10mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[jws, jcamp, txt]]></content><html><![CDATA[<b>Data Type:</b> UV-VIS<br />
jws, jcamp, txt<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;
]]></html><datestamp>2008-10-17T12:17:34+01:00</datestamp><timestamp>2008-10-20T16:03:42+01:00</timestamp><blog>5</blog><key>458ca9609dde1fa076af9252c6eda454</key><metadata><data_type>UV-VIS</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/18.xml</data><data>http://biolab.isis.rl.ac.uk/data/24.xml</data><data>http://biolab.isis.rl.ac.uk/data/56.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/46</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/153/UVVis_spectrum_of_10mgmL_Lysozyme.png</format></formats><comments/></post><post><id>150</id><rid>203</rid><title>UV-Vis spectrum of 2mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[jws file, jcamp, txt]]></content><html><![CDATA[<b>Data Type:</b> UV-VIS<br />
jws file, jcamp, txt<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;
]]></html><datestamp>2008-10-17T12:16:37+01:00</datestamp><timestamp>2008-10-20T16:04:13+01:00</timestamp><blog>5</blog><key>a4bd306a1bded4d1206b63a98a2c726d</key><metadata><data_type>UV-VIS</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/16.xml</data><data>http://biolab.isis.rl.ac.uk/data/26.xml</data><data>http://biolab.isis.rl.ac.uk/data/58.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/45</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/150/UVVis_spectrum_of_2mgmL_GFP.png</format></formats><comments/></post><post><id>147</id><rid>205</rid><title>UV-Vis spectrum of 5mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[jws file, jcamp,txt]]></content><html><![CDATA[<b>Data Type:</b> UV-VIS<br />
jws file, jcamp,txt<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;
]]></html><datestamp>2008-10-17T12:14:10+01:00</datestamp><timestamp>2008-10-20T16:04:46+01:00</timestamp><blog>5</blog><key>100180e9674e46e91befdc98268451e3</key><metadata><data_type>UV-VIS</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/14.xml</data><data>http://biolab.isis.rl.ac.uk/data/28.xml</data><data>http://biolab.isis.rl.ac.uk/data/60.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/44</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/147/UVVis_spectrum_of_5mgmL_GFP.png</format></formats><comments/></post><post><id>144</id><rid>207</rid><title>UV-Vis spectrum of 10mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[JWS file, jcamp, txt]]></content><html><![CDATA[<b>Data Type:</b> UV-VIS<br />
JWS file, jcamp, txt<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/171/UVVis_spectroscopy_of_GFP_and_Lysozyme_samples.html">UV-Vis spectroscopy of GFP and Lysozyme samples</a>;
]]></html><datestamp>2008-10-17T12:10:13+01:00</datestamp><timestamp>2008-10-20T16:05:22+01:00</timestamp><blog>5</blog><key>f5743fea2f674a7a7f8ce832fefff1f4</key><metadata><data_type>UV-VIS</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/12.xml</data><data>http://biolab.isis.rl.ac.uk/data/30.xml</data><data>http://biolab.isis.rl.ac.uk/data/62.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/43</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/144/UVVis_spectrum_of_10mgmL_GFP.png</format></formats><comments/></post><post><id>142</id><rid>208</rid><title>SANS on GFP concentration series</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length banjo cells cell at a temperature of 20 C. The data were reduced using Collette and the buffer as a background subtraction.
[table]
[row]Sample[col]Exposure time (min)[col]Run #[col]Trans Run#[col]Data[/row]
[row][blog]141[/blog][col]60[col]44699[col]44687[col][/row]
[row][blog]131[/blog][col]60[col]44700[col]44688[col][blog]174[/blog][/row]
[row][blog]132[/blog][col]60[col]44701[col]44689[col][blog]178[/blog][/row]
[row][blog]133[/blog][col]60[col]44702[col]44690[col][blog]182[/blog][/row]
[row][blog]137[/blog][col]60[col]44703[col]44691[col][blog]186[/blog][/row]
[row][blog]138[/blog][col]60[col]44704[col]44692[col][blog]190[/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length banjo cells cell at a temperature of 20 C. The data were reduced using Collette and the buffer as a background subtraction.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Exposure time (min)</td><td class="table_st">Run #</td><td class="table_st">Trans Run#</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/141/20_mM_Phosphate_buffer_in_D2O.html">20 mM Phosphate buffer in D2O</a></td><td class="table_st">60</td><td class="table_st">44699</td><td class="table_st">44687</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/131/GFP_10_mgmL_in_D2O_buffer.html">GFP (10 mg/mL) in D2O buffer</a></td><td class="table_st">60</td><td class="table_st">44700</td><td class="table_st">44688</td><td class="table_st"><a href="camerons_labblog/174/44700__10mgmL_GFP_on_LOQ.html">44700 - 10mg/mL GFP on LOQ</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/132/GFP_5_mgmL_in_D2O_buffer.html">GFP 5 mg/mL in D2O buffer</a></td><td class="table_st">60</td><td class="table_st">44701</td><td class="table_st">44689</td><td class="table_st"><a href="camerons_labblog/178/44701__5mgmL_GFP_on_LOQ.html">44701 - 5mg/mL GFP on LOQ</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/133/GFP_2_mgmL_in_D2O_buffer.html">GFP 2 mg/mL in D2O buffer</a></td><td class="table_st">60</td><td class="table_st">44702</td><td class="table_st">44690</td><td class="table_st"><a href="camerons_labblog/182/44702__2mgmL_GFP_on_LOQ.html">44702 - 2mg/mL GFP on LOQ</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/137/Lysozyme_10_mgmL_in_D2O_buffer.html">Lysozyme 10 mg/mL in D2O buffer</a></td><td class="table_st">60</td><td class="table_st">44703</td><td class="table_st">44691</td><td class="table_st"><a href="camerons_labblog/186/44703__10mgmL_Lysozyme_on_LOQ.html">44703 - 10mg/mL Lysozyme on LOQ</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/138/Lysozyme_2mgmL.html">Lysozyme 2mg/mL</a></td><td class="table_st">60</td><td class="table_st">44704</td><td class="table_st">44692</td><td class="table_st"><a href="camerons_labblog/190/44704__2mgmL_Lysozyme_on_LOQ.html">44704 - 2mg/mL Lysozyme on LOQ</a></td></tr></table><br/>
]]></html><datestamp>2008-10-16T15:52:49+01:00</datestamp><timestamp>2008-10-20T16:11:52+01:00</timestamp><blog>5</blog><key>676a9e02de155a7fe68dc681a1c28852</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/42</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/142/SANS_on_GFP_concentration_series.png</format></formats><comments/></post><post><id>109</id><rid>209</rid><title>SAXS of GFP Samples</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on the SAXSess instrument in the Edler group at the University of Bath. The samples were run in the capillary cell at a temperature of 25 C. Data was collected on the image plate provided and read using ImageQuant software provided with the image plate reader. The image was converted to 1D scattering data (I vs Q) using the SAXS Quant software provided.
[table]
[row]Sample[col]Exposure time (min)[col]Run #[col]Data[/row]
[row][blog]76[/blog][col]30[col]02067[col][blog]91[/blog][/row]
[row][blog]81[/blog][col]30[col]02068[col][blog]92[/blog][/row]
[row][blog]81[/blog][col]30[col]02069[col][blog]93[/blog][/row]
[row][blog]81[/blog][col]30[col]02070[col][blog]94[/blog][/row]
[row][blog]83[/blog][col]60[col]02071[col][blog]95[/blog][/row]
[row][blog]86[/blog][col]30[col]02072[col][blog]96[/blog][/row]
[row][blog]88[/blog][col]30[col]02073[col][blog]97[/blog][/row]
[row][blog]87[/blog][col]60[col]02074[col][blog]108[/blog][/row]
[row][blog]76[/blog][col]30[col]02075[col][blog]110[/blog][/row]
[/table]
Run #02069 was aborted because the image plate was misaligned in the image plate carousel. There is therefore no data file recorded.]]></content><html><![CDATA[<b>Procedure:</b> SAXS_Lab<br />
<b>Instrument:</b> SAXSess_Bath<br />
The following samples were run as described on the SAXSess instrument in the Edler group at the University of Bath. The samples were run in the capillary cell at a temperature of 25 C. Data was collected on the image plate provided and read using ImageQuant software provided with the image plate reader. The image was converted to 1D scattering data (I vs Q) using the SAXS Quant software provided.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Exposure time (min)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/76/Sortase_Buffer.html">Sortase Buffer</a></td><td class="table_st">30</td><td class="table_st">02067</td><td class="table_st"><a href="camerons_labblog/91/SAXS_Run_02067.html">SAXS Run 02067</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a></td><td class="table_st">30</td><td class="table_st">02068</td><td class="table_st"><a href="camerons_labblog/92/SAXS_Run_02068.html">SAXS Run 02068</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a></td><td class="table_st">30</td><td class="table_st">02069</td><td class="table_st"><a href="camerons_labblog/93/SAXS_Run_02069.html">SAXS Run 02069</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a></td><td class="table_st">30</td><td class="table_st">02070</td><td class="table_st"><a href="camerons_labblog/94/SAXS_Run_02070.html">SAXS Run 02070</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/83/GFP_solution_1_mgml.html">GFP solution 1 mg/ml</a></td><td class="table_st">60</td><td class="table_st">02071</td><td class="table_st"><a href="camerons_labblog/95/SAXS_Run_02071.html">SAXS Run 02071</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/86/Centrifuged_10_mgmL_GFP.html">Centrifuged 10 mg/mL GFP</a></td><td class="table_st">30</td><td class="table_st">02072</td><td class="table_st"><a href="camerons_labblog/96/SAXS_Run_02072.html">SAXS Run 02072</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/88/Centrifuged_5_mgmL_GFP.html">Centrifuged 5 mg/mL GFP</a></td><td class="table_st">30</td><td class="table_st">02073</td><td class="table_st"><a href="camerons_labblog/97/SAXS_Run_02073.html">SAXS Run 02073</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/87/Centrifuged_2_mgmL_GFP.html">Centrifuged 2 mg/mL GFP</a></td><td class="table_st">60</td><td class="table_st">02074</td><td class="table_st"><a href="camerons_labblog/108/SAXS_Run_02074.html">SAXS Run 02074</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/76/Sortase_Buffer.html">Sortase Buffer</a></td><td class="table_st">30</td><td class="table_st">02075</td><td class="table_st"><a href="camerons_labblog/110/SAXS_Run_02075.html">SAXS Run 02075</a></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>Run #02069 was aborted because the image plate was misaligned in the image plate carousel. There is therefore no data file recorded.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>;
]]></html><datestamp>2008-10-09T18:14:20+01:00</datestamp><timestamp>2008-10-20T16:12:50+01:00</timestamp><blog>5</blog><key>78a2af9140bece87c082f8e2ac1c874d</key><metadata><procedure>SAXS_Lab</procedure><instrument>SAXSess_Bath</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/35</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/109/SAXS_of_GFP_Samples.png</format></formats><comments/></post><post><id>210</id><rid>217</rid><title>oligo template</title><section>Templates</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col][[box]][/row]
[row]Number[col][[box]][/row]
[row]Location[col][[box]][/row]
[row]Sequence[col][[box]][/row]
[row]Length[col][[box]][/row]
[row]Melting temp[col][[box]][/row]
[row]Supplier[col][[box]][/row]
[row]Stock concentration[col][[box]][/row][/table]
[[Section>Materials]]
[[DNA>oligonucleotide]]
[[Material>solution]]]]></content><html><![CDATA[<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Number</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Location</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Length</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Supplier</td><td class="table_st">[[box]]</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st">[[box]]</td></tr></table><br style="clear:left;"/><br style="clear:left;"/>[[Section>Materials]]<br style="clear:left;"/>[[DNA>oligonucleotide]]<br style="clear:left;"/>[[Material>solution]]<br/>
]]></html><datestamp>2008-10-27T13:09:49+00:00</datestamp><timestamp>2008-10-27T13:45:50+00:00</timestamp><blog>5</blog><key>1638fb8d63ae6aac1aabc45f61eb59ed</key><links><uri>http://biolab.isis.rl.ac.uk/uri/4f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/210/oligo_template.png</format></formats><comments/></post><post><id>231</id><rid>234</rid><title>Planning preparation of DNA for Laser Tweezers experiment</title><section>Note</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The background to this experiment is given in my old lab book here:
http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/18571
As described the presumed final volume for the ligations reactions is the 50uL given in the supplementary information for this paper: [pubmed]12947199[/pubmed]. There is sufficient DNA for each of the oligos to make 100 uM stocks which will make it easy to add relatively small volumes for each ligation step.
The overall plan is therefore to make 100 uM stocks of the oligos in straight Tris buffer. For each oligo I will then do a kinase reaction at ~100 uM oligo (i.e. by just adding the reagents). The [blog]228[/blog] is 0.33 ug/uL which is very close to 1x10<sup>-8</sup> M as required by the described method. Unfortunately it is in a Tris-EDTA buffer which will probably interfere with the kinase and ligation reactions. I will therefore take 100 uL and ethanol ppt it as Luke has recently made up buffers for this.
The ligation of the first (biotin end) then follows by mixing the lambda (10<sup>-8</sup> M) with [blog]224[/blog] (10<sup>-7</sup> M) and annealing followed by ligation. This mixture is then combined with the appropriate combination of end oligos ([blog]221[/blog] and [blog]218[/blog] or [blog]214[/blog] and[blog]211[/blog] at 10<sup>-6</sup> M) followed by annealing and ligation.
As the amount of biotinylated lamda we want is relatively small contamination with the biotin oligo shouldn't be a problem. If this seems to cause issues we can easily ethanol ppt during the experiment.
I think it will be advisable to get fresh PNK and ligase for this along with fresh ATP to rule out any problems with the enzymes. These are now ordered so we are right to go tomorrow or Thursday. Will attempt to sort out ethanol ppt of lambda this afternoon and make sure buffers are together.]]></content><html><![CDATA[The background to this experiment is given in my old lab book here:<br style="clear:left;"/><a href="http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/18571">http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/18571</a><br style="clear:left;"/><br style="clear:left;"/>As described the presumed final volume for the ligations reactions is the 50uL given in the supplementary information for this paper: <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12947199" target=_blank class=ext_link>PMID: 12947199</a>. There is sufficient DNA for each of the oligos to make 100 uM stocks which will make it easy to add relatively small volumes for each ligation step.<br style="clear:left;"/><br style="clear:left;"/>The overall plan is therefore to make 100 uM stocks of the oligos in straight Tris buffer. For each oligo I will then do a kinase reaction at ~100 uM oligo (i.e. by just adding the reagents). The <a href="lab_materials/228/Lambda_DNA.html">Lambda DNA</a> is 0.33 ug/uL which is very close to 1x10<sup>-8</sup> M as required by the described method. Unfortunately it is in a Tris-EDTA buffer which will probably interfere with the kinase and ligation reactions. I will therefore take 100 uL and ethanol ppt it as Luke has recently made up buffers for this.<br style="clear:left;"/><br style="clear:left;"/>The ligation of the first (biotin end) then follows by mixing the lambda (10<sup>-8</sup> M) with <a href="camerons_labblog/224/Oligolambdabiotin.html">Oligo-lambda-biotin</a> (10<sup>-7</sup> M) and annealing followed by ligation. This mixture is then combined with the appropriate combination of end oligos (<a href="camerons_labblog/221/OligoTerRlam.html">Oligo-TerRlam</a> and <a href="camerons_labblog/218/OligoTerR.html">Oligo-TerR</a> or <a href="camerons_labblog/214/OligoTerFlam.html">Oligo-TerFlam</a> and<a href="camerons_labblog/211/OligoTerF.html">Oligo-TerF</a> at 10<sup>-6</sup> M) followed by annealing and ligation.<br style="clear:left;"/><br style="clear:left;"/>As the amount of biotinylated lamda we want is relatively small contamination with the biotin oligo shouldn't be a problem. If this seems to cause issues we can easily ethanol ppt during the experiment.<br style="clear:left;"/><br style="clear:left;"/>I think it will be advisable to get fresh PNK and ligase for this along with fresh ATP to rule out any problems with the enzymes. These are now ordered so we are right to go tomorrow or Thursday. Will attempt to sort out ethanol ppt of lambda this afternoon and make sure buffers are together.<br/>
]]></html><datestamp>2008-10-27T15:01:53+00:00</datestamp><timestamp>2008-10-28T12:12:57+00:00</timestamp><blog>5</blog><key>23e3763280db34015c5bce0c08b817e1</key><links><uri>http://biolab.isis.rl.ac.uk/uri/57</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.png</format></formats><comments/></post><post><id>240</id><rid>242</rid><title>Ethanol precipitated lambda DNA</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Lambda DNA resuspended in nuclease free water produced in [blog]239[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
Lambda DNA resuspended in nuclease free water produced in <a href="camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.html">Ethanol precipitation of lambda DNA</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;<a href="camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.html">Ethanol precipitation of lambda DNA</a>;
]]></html><datestamp>2008-10-28T15:35:18+00:00</datestamp><timestamp>2008-10-28T15:37:39+00:00</timestamp><blog>5</blog><key>c07cb6713f9f2895994f7f1ff302d03c</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/5c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/240/Ethanol_precipitated_lambda_DNA.png</format></formats><comments/></post><post><id>263</id><rid>263</rid><title>Phosphorylated TerR lam</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Prepared in [blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> oligonucleotide<br />
Prepared in <a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;<a href="camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.html">First preparation of beads for laser tweezers experiment</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:02:10+00:00</datestamp><timestamp>2008-11-05T16:02:10+00:00</timestamp><blog>5</blog><key>0f354bb608239d3e912774be394d33bf</key><metadata><material>Solution</material><dna>oligonucleotide</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/67</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/263/Phosphorylated_TerR_lam.png</format></formats><comments/></post><post><id>264</id><rid>264</rid><title>Phosporylated TerR</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Prepared in [blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> oligonucleotide<br />
Prepared in <a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:02:43+00:00</datestamp><timestamp>2008-11-05T16:02:43+00:00</timestamp><blog>5</blog><key>e06c845b4981f3a16964a83001feaaca</key><metadata><material>Solution</material><dna>oligonucleotide</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/68</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/264/Phosporylated_TerR.png</format></formats><comments/></post><post><id>265</id><rid>268</rid><title>Phosphorylated TerFlam</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Prepared in[blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> oligonucleotide<br />
Prepared in<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:03:13+00:00</datestamp><timestamp>2008-11-05T16:04:45+00:00</timestamp><blog>5</blog><key>8349da83b21c84c3f50ddf46e73e8403</key><metadata><material>Solution</material><dna>oligonucleotide</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/69</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/265/Phosphorylated_TerFlam.png</format></formats><comments/></post><post><id>269</id><rid>269</rid><title>Phosphorylated oligo-lambda-biotin</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[prepared in[blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> oligonucleotide<br />
prepared in<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html">Ligation of oligo-biotin-lambda to lambda DNA</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:05:58+00:00</datestamp><timestamp>2008-11-05T16:05:58+00:00</timestamp><blog>5</blog><key>a2fba022fee57b22f8a5616b1fcbc3b5</key><metadata><material>Solution</material><dna>oligonucleotide</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/6b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/269/Phosphorylated_oligolambdabiotin.png</format></formats><comments/></post><post><id>275</id><rid>275</rid><title>Product of ligation of lambda to lambda-oligo-biotin</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The product of [blog]273[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> double_stranded_linear<br />
The product of <a href="camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html">Ligation of oligo-biotin-lambda to lambda DNA</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html">Ligation of oligo-biotin-lambda to lambda DNA</a>;<a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;
]]></html><datestamp>2008-11-05T17:08:20+00:00</datestamp><timestamp>2008-11-05T17:08:20+00:00</timestamp><blog>5</blog><key>509e19551e60cfd8443d68879e69d98b</key><metadata><material>Solution</material><dna>double_stranded_linear</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/6f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.png</format></formats><comments/></post><post><id>262</id><rid>276</rid><title>Phosphorylated lambda DNA</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Phosphorylated lambda DNA prepared in [blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> double_stranded_linear<br />
Phosphorylated lambda DNA prepared in <a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html">Ligation of oligo-biotin-lambda to lambda DNA</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:01:05+00:00</datestamp><timestamp>2008-11-05T17:08:39+00:00</timestamp><blog>5</blog><key>da7fcf88bcd3ef5c45f38631f7c51124</key><metadata><material>Solution</material><dna>double_stranded_linear</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/66</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/262/Phosphorylated_lambda_DNA.png</format></formats><comments/></post><post><id>273</id><rid>277</rid><title>Ligation of oligo-biotin-lambda to lambda DNA</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[blog]262[/blog] (250 uL) and [blog]269[/blog] (0.5 uL) were combined and heated to 50 C followed by slow cooling over one hour. To this solution was added [blog]272[/blog] (1 uL) and the solution incubated overnight at 16 C to give [blog]275[/blog].
This procedure follows that in [pubmed]12947199[/pubmed] (supplementary information) except for a longer ligation period.]]></content><html><![CDATA[<b>Procedure:</b> DNA_ligation<br />
<a href="camerons_labblog/262/Phosphorylated_lambda_DNA.html">Phosphorylated lambda DNA</a> (250 uL) and <a href="camerons_labblog/269/Phosphorylated_oligolambdabiotin.html">Phosphorylated oligo-lambda-biotin</a> (0.5 uL) were combined and heated to 50 C followed by slow cooling over one hour. To this solution was added <a href="lab_materials/272/5_x_T4_DNA_ligase_buffer.html">5 x T4 DNA ligase buffer</a> (1 uL) and the solution incubated overnight at 16 C to give <a href="camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html">Product of ligation of lambda to lambda-oligo-biotin</a>. <br style="clear:left;"/><br style="clear:left;"/>This procedure follows that in <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=12947199" target=_blank class=ext_link>PMID: 12947199</a> (supplementary information) except for a longer ligation period.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html">Product of ligation of lambda to lambda-oligo-biotin</a>;
]]></html><datestamp>2008-11-05T17:06:34+00:00</datestamp><timestamp>2008-11-05T17:29:54+00:00</timestamp><blog>5</blog><key>05308b7374d0fab7fd0173ec769c3792</key><metadata><procedure>DNA_ligation</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/6e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/273/Ligation_of_oligobiotinlambda_to_lambda_DNA.png</format></formats><comments/></post><post><id>266</id><rid>278</rid><title>Phosphorylated TerF</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Prepared in[blog]261[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> oligonucleotide<br />
Prepared in<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-05T16:03:43+00:00</datestamp><timestamp>2008-11-05T17:30:52+00:00</timestamp><blog>5</blog><key>10a0a8b6e2dbc76f06e2ee4d176def7c</key><metadata><material>Solution</material><dna>oligonucleotide</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/6a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/266/Phosphorylated_TerF.png</format></formats><comments/></post><post><id>224</id><rid>279</rid><title>Oligo-lambda-biotin</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]lambda-biotin[/row]
[row]Number[col][/row]
[row]Location[col]Freezer 1 - Cameron's box[/row]
[row]Sequence[col]5'-Agg TCg CCg CCC AAA AAA AAA AAA AAA AAA AA-biotin-3'[/row]
[row]Length[col]32[/row]
[row]Melting temp[col][/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col][/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">lambda-biotin</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st">Freezer 1 - Cameron's box</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">5'-Agg TCg CCg CCC AAA AAA AAA AAA AAA AAA AA-biotin-3'</td></tr><tr><td class="table_st">Length</td><td class="table_st">32</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st"></td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st"></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html">Planning preparation of DNA for Laser Tweezers experiment</a>;<a href="camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.html">Preparing fresh polymer-DNA beads for lasers experiment</a>;<a href="camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.html">Examining silica beads for stickiness to surface</a>;<a href="shubbies_lablog/447/Polymer_beads_with_low_loading_of_lambdabiotinDNA.html">Polymer beads with low loading of lambda-biotin-DNA</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-10-27T14:14:48+00:00</datestamp><timestamp>2008-11-05T17:31:13+00:00</timestamp><blog>5</blog><key>56e121e1f56bbe3139b745b48c8fb340</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/72.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/54</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/224/Oligolambdabiotin.png</format></formats><comments/></post><post><id>221</id><rid>280</rid><title>Oligo-TerRlam</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]TerRlam[/row]
[row]Number[col][/row]
[row]Location[col]Freezer 1 - Cameron's box[/row]
[row]Sequence[col]ggg Cgg CgA CCTATAAGTATGTTGTAACTAAAG[/row]
[row]Length[col]33[/row]
[row]Melting temp[col][/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col][/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">TerRlam</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st">Freezer 1 - Cameron's box</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">ggg Cgg CgA CCTATAAGTATGTTGTAACTAAAG</td></tr><tr><td class="table_st">Length</td><td class="table_st">33</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st"></td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st"></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html">Planning preparation of DNA for Laser Tweezers experiment</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-10-27T13:48:01+00:00</datestamp><timestamp>2008-11-05T17:31:31+00:00</timestamp><blog>5</blog><key>588f4a854c7099e2e04456d8b725724b</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/70.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/53</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/221/OligoTerRlam.png</format></formats><comments/></post><post><id>218</id><rid>281</rid><title>Oligo-TerR</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]TerR[/row]
[row]Number[col][/row]
[row]Location[col]Freezer 1 - Cameron's box[/row]
[row]Sequence[col]CTTTAGTTACAACATACTTAT[/row]
[row]Length[col]21[/row]
[row]Melting temp[col][/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col][/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">TerR</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st">Freezer 1 - Cameron's box</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">CTTTAGTTACAACATACTTAT</td></tr><tr><td class="table_st">Length</td><td class="table_st">21</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st"></td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st"></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html">Planning preparation of DNA for Laser Tweezers experiment</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-10-27T13:46:50+00:00</datestamp><timestamp>2008-11-05T17:31:52+00:00</timestamp><blog>5</blog><key>68973ced2a72fc9864763b921e8ca7f3</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/68.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/52</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/218/OligoTerR.png</format></formats><comments/></post><post><id>214</id><rid>282</rid><title>Oligo-TerFlam</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]TerFlam[/row]
[row]Number[col][/row]
[row]Location[col]Freezer 1 - Cameron's box[/row]
[row]Sequence[col]ggg Cgg CgA CCT CTTTAGTTACAACATACTTAT[/row]
[row]Length[col]33[/row]
[row]Melting temp[col][/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col][/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">TerFlam</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st">Freezer 1 - Cameron's box</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">ggg Cgg CgA CCT CTTTAGTTACAACATACTTAT</td></tr><tr><td class="table_st">Length</td><td class="table_st">33</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st"></td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st"></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html">Planning preparation of DNA for Laser Tweezers experiment</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-10-27T13:44:58+00:00</datestamp><timestamp>2008-11-05T17:32:16+00:00</timestamp><blog>5</blog><key>f16fbbd01b399a75fe1ba85af0520809</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/66.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/51</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/214/OligoTerFlam.png</format></formats><comments/></post><post><id>211</id><rid>283</rid><title>Oligo-TerF</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]TerF[/row]
[row]Number[col][/row]
[row]Location[col]Freezer 1 - Cameron's box[/row]
[row]Sequence[col]ATAAGTATGTTGTAACTAAAG[/row]
[row]Length[col]21[/row]
[row]Melting temp[col][/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col][/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">TerF</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st">Freezer 1 - Cameron's box</td></tr><tr><td class="table_st">Sequence</td><td class="table_st">ATAAGTATGTTGTAACTAAAG</td></tr><tr><td class="table_st">Length</td><td class="table_st">21</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st"></td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st"></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/231/Planning_preparation_of_DNA_for_Laser_Tweezers_experiment.html">Planning preparation of DNA for Laser Tweezers experiment</a>;<a href="camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html">Phosphorylation of DNA substrates for laser tweezers experiment</a>;
]]></html><datestamp>2008-10-27T13:41:00+00:00</datestamp><timestamp>2008-11-05T17:32:48+00:00</timestamp><blog>5</blog><key>c4720d14f49f328ae45fdd762ed50001</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/64.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/50</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/211/OligoTerF.png</format></formats><comments/></post><post><id>76</id><rid>284</rid><title>Sortase Buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Prepared by Maria Gomis-Gomis at the University of Southampton.
[table]
[mrow]Property[mcol]Data[/mrow]
[row]Name[col]Sortase buffer[/row]
[row]Expiry[col][/row]
[row]pH[col]7.5[/row]
[row]Tris-HCl[col]50 mM[/row]
[row]NaCl[col]100 mM[/row]
[row]CaCl2[col]5 mM[/row]
[/table]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
Prepared by Maria Gomis-Gomis at the University of Southampton. <br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">Sortase buffer</td></tr><tr><td class="table_st">Expiry</td><td class="table_st"></td></tr><tr><td class="table_st">pH</td><td class="table_st">7.5</td></tr><tr><td class="table_st">Tris-HCl</td><td class="table_st">50 mM</td></tr><tr><td class="table_st">NaCl</td><td class="table_st">100 mM</td></tr><tr><td class="table_st">CaCl2</td><td class="table_st">5 mM</td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/81/GFP_solution_5_mgml.html">GFP solution 5 mg/ml</a>;<a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;<a href="camerons_labblog/109/SAXS_of_GFP_Samples.html">SAXS of GFP Samples</a>;
]]></html><datestamp>2008-10-09T11:24:25+01:00</datestamp><timestamp>2008-11-05T17:33:08+00:00</timestamp><blog>5</blog><key>978a60a5043b41604e73716c574cc2b1</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/21</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/76/Sortase_Buffer.png</format></formats><comments/></post><post><id>78</id><rid>285</rid><title>GFP solution 10 mg/mL</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The solution of GFP prepared in [blog]79[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
The solution of GFP prepared in <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;
]]></html><datestamp>2008-10-09T11:30:01+01:00</datestamp><timestamp>2008-11-05T17:34:43+00:00</timestamp><blog>5</blog><key>7693cecaf1ce2d82a892ca74c195973b</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/23</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/78/GFP_solution_10_mgmL.png</format></formats><comments/></post><post><id>77</id><rid>286</rid><title>Freeze dried GFP</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[GFP prepared by Luke Clifton and Mark Telling. This has been purified by Ni-NTA chromatography but not further purified. A fluffy yellow powder. This specific sample given to Maria Gomis-Gomis at Southampton University for SAXS and MS experiments]]></content><html><![CDATA[<b>Material:</b> Solution<br />
GFP prepared by Luke Clifton and Mark Telling. This has been purified by Ni-NTA chromatography but not further purified. A fluffy yellow powder. This specific sample given to Maria Gomis-Gomis at Southampton University for SAXS and MS experiments<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/79/Preparation_of_GFP_solutions.html">Preparation of GFP solutions</a>;<a href="camerons_labblog/130/Preparation_of_GFP_Samples_for_a_quickie_SANS_experiment_on_LOQ.html">Preparation of GFP Samples for a quickie SANS experiment on LOQ</a>;
]]></html><datestamp>2008-10-09T11:26:29+01:00</datestamp><timestamp>2008-11-05T17:35:00+00:00</timestamp><blog>5</blog><key>84824d7f45eb1244a656f88f09415add</key><metadata><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/22</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/77/Freeze_dried_GFP.png</format></formats><comments/></post><post><id>287</id><rid>287</rid><title>1:100 gly-OH modified silica beads</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Silica beads]]></content><html><![CDATA[<b>Material:</b> Powder<br />
Silica beads<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a>;
]]></html><datestamp>2008-11-06T10:22:35+00:00</datestamp><timestamp>2008-11-06T10:22:35+00:00</timestamp><blog>5</blog><key>6f68f1c89bca7a211164105aacae2089</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/70</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/287/1100_glyOH_modified_silica_beads.png</format></formats><comments/></post><post><id>288</id><rid>288</rid><title>1:1000 gly-OH modified silica beads</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[beads]]></content><html><![CDATA[<b>Material:</b> Powder<br />
beads<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a>;
]]></html><datestamp>2008-11-06T10:23:26+00:00</datestamp><timestamp>2008-11-06T10:23:26+00:00</timestamp><blog>5</blog><key>8ec756f325088ccba987840212c4a679</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/71</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/288/11000_glyOH_modified_silica_beads.png</format></formats><comments/></post><post><id>289</id><rid>289</rid><title>1:10000 gly-OH modified silica beads</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[beads]]></content><html><![CDATA[<b>Material:</b> Powder<br />
beads<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a>;
]]></html><datestamp>2008-11-06T10:23:50+00:00</datestamp><timestamp>2008-11-06T10:23:50+00:00</timestamp><blog>5</blog><key>a107536cd93fc6a1aa0d3b979a719ae4</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/72</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/289/110000_glyOH_modified_silica_beads.png</format></formats><comments/></post><post><id>290</id><rid>290</rid><title>1:100000 gly-OH modified silica beads</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[beads]]></content><html><![CDATA[<b>Material:</b> Powder<br />
beads<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a>;
]]></html><datestamp>2008-11-06T10:24:40+00:00</datestamp><timestamp>2008-11-06T10:24:40+00:00</timestamp><blog>5</blog><key>d1e966f54e417407af955c2dc52a3472</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/73</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/290/1100000_glyOH_modified_silica_beads.png</format></formats><comments/></post><post><id>295</id><rid>296</rid><title>TerF-lambda-biotin construct</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Product #1 of [blog]294[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> double_stranded_linear<br />
Product #1 of <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;
]]></html><datestamp>2008-11-06T11:28:21+00:00</datestamp><timestamp>2008-11-06T11:28:42+00:00</timestamp><blog>5</blog><key>33bf2c8eef376771d7b30be23d5e7f64</key><metadata><material>Solution</material><dna>double_stranded_linear</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/76</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/295/TerFlambdabiotin_construct.png</format></formats><comments/></post><post><id>294</id><rid>299</rid><title>Ligation of Tus adaptors to biotin-modified lambda</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[To [blog]275[/blog] was added the adaptors as given below. The solutions were heated to 65 C and then cooled over approximately one hour. Fresh ATP (0.5 uL of 10 mM) and [blog]271[/blog] (1 uL of 1U/uL) were then added and the solutions incubated at 16 C for two hours.
[table]
[row]#[col][blog]275[/blog] (uL)[col]Adaptor 1[col]uL[col]Adaptor 2[col]uL[col]Product[/row]
[row]1[col]50[col][blog]266[/blog][col]0.5[col][blog]265[/blog][col]0.5[col][blog]295[/blog][/row]
[row]2[col]50[col][blog]264[/blog][col]0.5[col][blog]263[/blog][col]0.5[col][blog]298[/blog][/row]
[/table]
Note that Rxn #1 is actually Ter forward. It shows as TerR because of a mistake with the original title of the material posts.]]></content><html><![CDATA[<b>Procedure:</b> DNA_ligation<br />
To <a href="camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html">Product of ligation of lambda to lambda-oligo-biotin</a> was added the adaptors as given below. The solutions were heated to 65 C and then cooled over approximately one hour. Fresh ATP (0.5 uL of 10 mM) and <a href="lab_materials/271/T4_DNA_ligase.html">T4 DNA ligase</a> (1 uL of 1U/uL) were then added and the solutions incubated at 16 C for two hours.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">#</td><td class="table_st"><a href="camerons_labblog/275/Product_of_ligation_of_lambda_to_lambdaoligobiotin.html">Product of ligation of lambda to lambda-oligo-biotin</a> (uL)</td><td class="table_st">Adaptor 1</td><td class="table_st">uL</td><td class="table_st">Adaptor 2</td><td class="table_st">uL</td><td class="table_st">Product</td></tr><tr><td class="table_st">1</td><td class="table_st">50</td><td class="table_st"><a href="camerons_labblog/266/Phosphorylated_TerR.html">Phosphorylated TerR</a></td><td class="table_st">0.5</td><td class="table_st"><a href="camerons_labblog/265/Phosphorylated_TerRlam.html">Phosphorylated TerRlam</a></td><td class="table_st">0.5</td><td class="table_st"><a href="camerons_labblog/295/TerFlambdabiotin_construct.html">TerF-lambda-biotin construct</a></td></tr><tr><td class="table_st">2</td><td class="table_st">50</td><td class="table_st"><a href="camerons_labblog/264/Phosporylated_TerR.html">Phosporylated TerR</a></td><td class="table_st">0.5</td><td class="table_st"><a href="camerons_labblog/263/Phosphorylated_TerR_lam.html">Phosphorylated TerR lam</a></td><td class="table_st">0.5</td><td class="table_st"><a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>Note that Rxn #1 is actually Ter forward. It shows as TerR because of a mistake with the original title of the material posts.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/295/TerFlambdabiotin_construct.html">TerF-lambda-biotin construct</a>;<a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a>;
]]></html><datestamp>2008-11-06T11:14:54+00:00</datestamp><timestamp>2008-11-06T11:48:48+00:00</timestamp><blog>5</blog><key>a5b9176047db8c0ca2bc781e947e7382</key><metadata><procedure>DNA_ligation</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/75</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.png</format></formats><comments/></post><post><id>300</id><rid>300</rid><title>1:100 gly-oh beads after ammonia treatment</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Product #1 from [blog]291[/blog]]]></content><html><![CDATA[<b>Material:</b> Powder<br />
Product #1 from <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html">Fresh beads for Laser Tweezers experiments</a>;<a href="camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.html">First preparation of beads for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-06T11:53:11+00:00</datestamp><timestamp>2008-11-06T11:53:11+00:00</timestamp><blog>5</blog><key>f622e865987f526ece6a435da4a796f4</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/78</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.png</format></formats><comments/></post><post><id>301</id><rid>301</rid><title>1:1000 gly-OH beads after ammonia treatment</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Product #2 of [blog]291[/blog]]]></content><html><![CDATA[<b>Material:</b> Powder<br />
Product #2 of <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html">Fresh beads for Laser Tweezers experiments</a>;
]]></html><datestamp>2008-11-06T11:53:45+00:00</datestamp><timestamp>2008-11-06T11:53:45+00:00</timestamp><blog>5</blog><key>b87b8f263f1a2342bb1067002630d241</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/79</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.png</format></formats><comments/></post><post><id>302</id><rid>302</rid><title>1:10000 gly-OH beads after ammonia treatment</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Product #3 of[blog]291[/blog]]]></content><html><![CDATA[<b>Material:</b> Powder<br />
Product #3 of<a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html">Fresh beads for Laser Tweezers experiments</a>;
]]></html><datestamp>2008-11-06T11:54:16+00:00</datestamp><timestamp>2008-11-06T11:54:16+00:00</timestamp><blog>5</blog><key>f3bc0ca8ce63072270074731be0e4468</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/7a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.png</format></formats><comments/></post><post><id>303</id><rid>303</rid><title>1:100,000 gly-OH beads after ammonia treatment</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[product #4 of [blog]291[/blog]]]></content><html><![CDATA[<b>Material:</b> Powder<br />
product #4 of <a href="camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html">Ammonia treatment of gly modified beads</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html">Fresh beads for Laser Tweezers experiments</a>;
]]></html><datestamp>2008-11-06T11:54:46+00:00</datestamp><timestamp>2008-11-06T11:54:46+00:00</timestamp><blog>5</blog><key>b2df3625ee2d0541d159ff4a02353a66</key><metadata><material>Powder</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/7b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.png</format></formats><comments/></post><post><id>291</id><rid>304</rid><title>Ammonia treatment of gly modified beads</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A sample of the silica beads previously prepared were treated with aqueous ammonia (200 uL) for 30' at 65 C as given.
[table]
[row]Beads[col]Weight (mg)[col]Product[/row]
[row][blog]287[/blog][col]20.96[col][/row]
[row][blog]288[/blog][col]31.26[col][/row]
[row][blog]289[/blog][col]26.57[col][/row]
[row][blog]290[/blog][col]20.92[col][/row]
[/table]
After addition of the ammonia the beads rapidly dispersed. A quick spin seemed to show there was still some beads there so hopefully not dissolved.
Beads were washed five times with ~1 mL of water and then dried in the freeze drier. Beads seem to be ok after washing.]]></content><html><![CDATA[<b>Procedure:</b> Chemistry<br />
A sample of the silica beads previously prepared were treated with aqueous ammonia (200 uL) for 30' at 65 C as given.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Beads</td><td class="table_st">Weight (mg)</td><td class="table_st">Product</td></tr><tr><td class="table_st"><a href="camerons_labblog/287/1100_glyOH_modified_silica_beads.html">1:100 gly-OH modified silica beads</a></td><td class="table_st">20.96</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/288/11000_glyOH_modified_silica_beads.html">1:1000 gly-OH modified silica beads</a></td><td class="table_st">31.26</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/289/110000_glyOH_modified_silica_beads.html">1:10000 gly-OH modified silica beads</a></td><td class="table_st">26.57</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/290/1100000_glyOH_modified_silica_beads.html">1:100000 gly-OH modified silica beads</a></td><td class="table_st">20.92</td><td class="table_st"></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>After addition of the ammonia the beads rapidly dispersed. A quick spin seemed to show there was still some beads there so hopefully not dissolved.<br style="clear:left;"/><br style="clear:left;"/>Beads were washed five times with ~1 mL of water and then dried in the freeze drier. Beads seem to be ok after washing.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html">1:100 gly-oh beads after ammonia treatment</a>;<a href="camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.html">1:1000 gly-OH beads after ammonia treatment</a>;<a href="camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.html">1:10000 gly-OH beads after ammonia treatment</a>;<a href="camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.html">1:100,000 gly-OH beads after ammonia treatment</a>;
]]></html><datestamp>2008-11-06T10:27:35+00:00</datestamp><timestamp>2008-11-06T12:16:50+00:00</timestamp><blog>5</blog><key>888f4662662c26dbcbf809531934fd52</key><metadata><procedure>Chemistry</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/74</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/291/Ammonia_treatment_of_gly_modified_beads.png</format></formats><comments/></post><post><id>305</id><rid>307</rid><title>Maria's analysis of the GFP SAXS data</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Maria Gomis-Gomis has done some analysis of Rg and I(0) values from the SAXS data that is described in [blog]123[/blog].
The Rgs are broadly consistent with the SANS data for the 5mg/mL and 2 mg/mL samples. The I(0) values do not appear consistent suggesting issues with background subtraction that will need to be looked at.]]></content><html><![CDATA[<b>Procedure:</b> Data_analysis<br />
Maria Gomis-Gomis has done some analysis of Rg and I(0) values from the SAXS data that is described in <a href="camerons_labblog/123/GFP_SAXS_data_reduction.html">GFP SAXS data reduction</a>.<br style="clear:left;"/><br style="clear:left;"/>The Rgs are broadly consistent with the SANS data for the 5mg/mL and 2 mg/mL samples. The I(0) values do not appear consistent suggesting issues with background subtraction that will need to be looked at.<br/>
]]></html><datestamp>2008-11-07T11:23:34+00:00</datestamp><timestamp>2008-11-07T11:24:56+00:00</timestamp><blog>5</blog><key>d9c61b665d361af17a928f681ec1e400</key><metadata><procedure>Data_analysis</procedure></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/76.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/7c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/305/Marias_analysis_of_the_GFP_SAXS_data.png</format></formats><comments/></post><post><id>298</id><rid>314</rid><title>TerR-lambda-biotin construct</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Product #2 of [blog]294[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Dna:</b> double_stranded_linear<br />
Product #2 of <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/294/Ligation_of_Tus_adaptors_to_biotinmodified_lambda.html">Ligation of Tus adaptors to biotin-modified lambda</a>;<a href="camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.html">Preparing fresh polymer-DNA beads for lasers experiment</a>;<a href="camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.html">Examining silica beads for stickiness to surface</a>;<a href="shubbies_lablog/447/Polymer_beads_with_low_loading_of_lambdabiotinDNA.html">Polymer beads with low loading of lambda-biotin-DNA</a>;
]]></html><datestamp>2008-11-06T11:47:08+00:00</datestamp><timestamp>2008-11-11T15:48:39+00:00</timestamp><blog>5</blog><key>ce89fe18d50229b6e9d4b3e826e2d187</key><metadata><material>Solution</material><dna>double_stranded_linear</dna></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/77</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/298/TerRlambdabiotin_construct.png</format></formats><comments/></post><post><id>352</id><rid>352</rid><title>Bangs Labs streptavidin coated beads</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[to be filled in]]></content><html><![CDATA[<b>Material:</b> Suspension<br />
to be filled in<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.html">Preparing fresh polymer-DNA beads for lasers experiment</a>;<a href="camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.html">First preparation of beads for laser tweezers experiment</a>;
]]></html><datestamp>2008-11-12T14:10:53+00:00</datestamp><timestamp>2008-11-12T14:10:53+00:00</timestamp><blog>5</blog><key>d8d96935b67d252c34e54ac780614dc2</key><metadata><material>Suspension</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/8f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.png</format></formats><comments/></post><post><id>375</id><rid>380</rid><title>Preparing fresh polymer-DNA beads for lasers experiment</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[blog]352[/blog] (20 uL) were mixed with [blog]298[/blog] (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl.
[blog]352[/blog] (20 uL) were mixed with [blog]298[/blog] (1 uL) and [blog]224[/blog] (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl and then 3 x 100 mM NaCl.]]></content><html><![CDATA[<b>Project:</b> Laser_tweezers_tus<br />
<a href="camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.html">Bangs Labs streptavidin coated beads</a> (20 uL) were mixed with <a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a> (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl.<br style="clear:left;"/><br style="clear:left;"/><a href="camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.html">Bangs Labs streptavidin coated beads</a> (20 uL) were mixed with <a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a> (1 uL) and <a href="camerons_labblog/224/Oligolambdabiotin.html">Oligo-lambda-biotin</a> (1 uL) and incubated for 30 minutes. The beads were washed twice with 1 mL of 5 mM Tris-HCl pH 7.5, 1 M NaCl and then 3 x 100 mM NaCl.<br/>
]]></html><datestamp>2008-11-14T10:21:15+00:00</datestamp><timestamp>2008-11-14T12:14:36+00:00</timestamp><blog>5</blog><key>8fd45d9d3700674ffcc570cf51f55a61</key><metadata><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/9a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/375/Preparing_fresh_polymerDNA_beads_for_lasers_experiment.png</format></formats><comments/></post><post><id>379</id><rid>385</rid><title>Examining silica beads for stickiness to surface</title><section>Note</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A quick check on labelled 100:1 beads and 100,000:1 beads indicate that both are very sticky when suspended in water or buffer. it seems possible to disperse them from the top and catch a few which may be viable for experiments but a bit of a pain.
More comprehensive analysis:
In 100 mM all appear to be sticky and also sticking to themselves. Beads are forming aggregates much worse than they were before.
All beads sets are extremely sticky in 100 mM, 50 mM, 0mM NaCl buffer. Beads themselves are more sticky, probably due to being shaken overnight in water but aggregates seem loose so sonication should clear things up. Not much good for easy interaction studies though.
Polymer beads when settled seem to be possible to pull back off the surface with the tweezers. After 10-15 minutes it is still possible to pull polymer beads (with DNA) off the surface with the traps. After 30 minutes it is getting harder to find easily trappable beads but there are still plenty about.
But there seems to be significant differences between different cells. Sticking in some but not others. Beads treated with [blog]298[/blog] plus [blog]224[/blog] seem to be less prone to sticking than those just treated with [blog]298[/blog].]]></content><html><![CDATA[<b>Project:</b> Laser_tweezers_tus<br />
A quick check on labelled 100:1 beads and 100,000:1 beads indicate that both are very sticky when suspended in water or buffer. it seems possible to disperse them from the top and catch a few which may be viable for experiments but a bit of a pain.<br style="clear:left;"/><br style="clear:left;"/>More comprehensive analysis:<br style="clear:left;"/><br style="clear:left;"/>In 100 mM all appear to be sticky and also sticking to themselves. Beads are forming aggregates much worse than they were before.<br style="clear:left;"/><br style="clear:left;"/>All beads sets are extremely sticky in 100 mM, 50 mM, 0mM NaCl buffer. Beads themselves are more sticky, probably due to being shaken overnight in water but aggregates seem loose so sonication should clear things up. Not much good for easy interaction studies though.<br style="clear:left;"/><br style="clear:left;"/>Polymer beads when settled seem to be possible to pull back off the surface with the tweezers. After 10-15 minutes it is still possible to pull polymer beads (with DNA) off the surface with the traps. After 30 minutes it is getting harder to find easily trappable beads but there are still plenty about.<br style="clear:left;"/><br style="clear:left;"/>But there seems to be significant differences between different cells. Sticking in some but not others. Beads treated with <a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a> plus <a href="camerons_labblog/224/Oligolambdabiotin.html">Oligo-lambda-biotin</a> seem to be less prone to sticking than those just treated with <a href="camerons_labblog/298/TerRlambdabiotin_construct.html">TerR-lambda-biotin construct</a>.<br/>
]]></html><datestamp>2008-11-14T12:14:20+00:00</datestamp><timestamp>2008-11-14T17:12:14+00:00</timestamp><blog>5</blog><key>e7196b695b66f55c2c935b6480548205</key><metadata><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/9b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/379/Examining_silica_beads_for_stickiness_to_surface.png</format></formats><comments/></post><post><id>387</id><rid>388</rid><title>Octameric rck domain from MthK in D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Octameric rck domain in 20 mM Tris-pD 8.0, 250 mM NaCl, in D2O. Prepared by Luke by performing gel filtration at pH 8.0]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
Octameric rck domain in 20 mM Tris-pD 8.0, 250 mM NaCl, in D2O. Prepared by Luke by performing gel filtration at pH 8.0<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/402/SANS_runs_on_octameric_RCK.html">SANS runs on octameric RCK</a>;<a href="camerons_labblog/405/Additional_runs_on_octameric_Rck.html">Additional runs on octameric Rck</a>;<a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a>;<a href="camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html">Dialysis of Octameric RCK samples</a>;
]]></html><datestamp>2008-11-16T11:21:24+00:00</datestamp><timestamp>2008-11-16T11:21:52+00:00</timestamp><blog>5</blog><key>4b8da4794fd6123a894a6978b6fd1c1e</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/9d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.png</format></formats><comments/></post><post><id>140</id><rid>398</rid><title>SANS on LOQ template</title><section>Templates</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[row][[Material:Solution]][col][[box=2]][col][[Box=5]][col][[Box=5]][col][[Blog]][/row]
[/table]
[[Section>Procedures]]
[[Procedure>SANS]]
[[Instrument>LOQ]]]]></content><html><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr><tr><td class="table_st">[[Material:Solution]]</td><td class="table_st">[[box=2]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Box=5]]</td><td class="table_st">[[Blog]]</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/>[[Section>Procedures]]<br style="clear:left;"/>[[Procedure>SANS]]<br style="clear:left;"/>[[Instrument>LOQ]]<br/>
]]></html><datestamp>2008-10-16T15:51:26+01:00</datestamp><timestamp>2008-11-16T12:42:38+00:00</timestamp><blog>5</blog><key>2d587f98ce20238e1ff3eb9cc54e04e2</key><links><uri>http://biolab.isis.rl.ac.uk/uri/40</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/140/SANS_on_LOQ_template.png</format></formats><comments/></post><post><id>394</id><rid>401</rid><title>SANS on LOQ template</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure (uamp.hr)[col]Run #[col]Data[/row]
[row]LOQ T49 Standard 14 mm[col]T[col]10[col]46281[col][blog][/blog][/row]
[row]LOQ standard Can 14 mm[col]T[col]10[col]46282[col][blog][/blog][/row]
[row]Direct beam[col]T[col]10[col]46283[col][blog][/blog][/row]
[row]LOQ T49 Standard 14 mm[col]S[col]15[col]46284[col][blog][/blog][/row]
[row]LOQ standard Can 14 mm[col]S[col]15[col]46285[col][blog][/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st">LOQ T49 Standard 14 mm</td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46281</td><td class="table_st"></td></tr><tr><td class="table_st">LOQ standard Can 14 mm</td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46282</td><td class="table_st"></td></tr><tr><td class="table_st">Direct beam</td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46283</td><td class="table_st"></td></tr><tr><td class="table_st">LOQ T49 Standard 14 mm</td><td class="table_st">S</td><td class="table_st">15</td><td class="table_st">46284</td><td class="table_st"></td></tr><tr><td class="table_st">LOQ standard Can 14 mm</td><td class="table_st">S</td><td class="table_st">15</td><td class="table_st">46285</td><td class="table_st"></td></tr></table><br/>
]]></html><datestamp>2008-11-16T12:38:41+00:00</datestamp><timestamp>2008-11-16T12:45:55+00:00</timestamp><blog>5</blog><key>d8c995003b88a4bc3aae88cd07bcbfad</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/82.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/9f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/394/SANS_on_LOQ_template.png</format></formats><comments/></post><post><id>402</id><rid>404</rid><title>SANS runs on octameric RCK</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][blog]387[/blog][col]T[col]10[col]46286[col][blog][/blog][/row]
[row][blog]390[/blog][col]T[col]10[col]46287[col][blog][/blog][/row]
[row][blog]387[/blog][col]S[col]85[col]46288[col][blog][/blog][/row]
[row][blog]390[/blog][col]S[col]85[col]46289[col][blog][/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html">Octameric rck domain from MthK in D2O buffer</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46286</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html">Buffer background for octameric rck domain</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46287</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html">Octameric rck domain from MthK in D2O buffer</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46288</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html">Buffer background for octameric rck domain</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46289</td><td class="table_st"></td></tr></table><br/>
]]></html><datestamp>2008-11-16T13:29:40+00:00</datestamp><timestamp>2008-11-16T13:30:12+00:00</timestamp><blog>5</blog><key>917c66865caf9ad4138621894c8e1b37</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/84.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/a0</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/402/SANS_runs_on_octameric_RCK.png</format></formats><comments/></post><post><id>405</id><rid>407</rid><title>Additional runs on octameric Rck</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][blog]387[/blog][col]S[col]85[col]46290[col][blog][/blog][/row]
[row][blog]390[/blog][col]S[col]85[col]46291[col][blog][/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html">Octameric rck domain from MthK in D2O buffer</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46290</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html">Buffer background for octameric rck domain</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46291</td><td class="table_st"></td></tr></table><br/>
]]></html><datestamp>2008-11-16T15:57:06+00:00</datestamp><timestamp>2008-11-16T15:57:49+00:00</timestamp><blog>5</blog><key>8efc50f30b73885389040d482a9128a6</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/86.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/a1</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/405/Additional_runs_on_octameric_Rck.png</format></formats><comments/></post><post><id>409</id><rid>409</rid><title>Octameric RCK plus Ca</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The product of adding Ca to octameric RCK domain in [blog]408[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
The product of adding Ca to octameric RCK domain in <a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a>;<a href="camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.html">SANS of octameric RCK plus Ca++</a>;<a href="camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html">Dialysis of Octameric RCK samples</a>;
]]></html><datestamp>2008-11-16T17:38:22+00:00</datestamp><timestamp>2008-11-16T17:38:22+00:00</timestamp><blog>5</blog><key>6d655284c48c88bb74e99cd332bbfe14</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a3</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/409/Octameric_RCK_plus_Ca.png</format></formats><comments/></post><post><id>410</id><rid>410</rid><title>Buffer background for octameric RCK plus Ca</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The product of adding Ca to buffer background in [blog]408[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
The product of adding Ca to buffer background in <a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a>;<a href="camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.html">SANS of octameric RCK plus Ca++</a>;
]]></html><datestamp>2008-11-16T17:40:07+00:00</datestamp><timestamp>2008-11-16T17:40:07+00:00</timestamp><blog>5</blog><key>a904b86e09cbd74c58109caa9a51b53d</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a4</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.png</format></formats><comments/></post><post><id>408</id><rid>415</rid><title>Addition of Ca++ to octameric mthk sample</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[To each of [blog]387[/blog] and [blog]390[/blog] (500 uL) in 1 mm SANS Tank cells was added CaCl2 (15 uL of a 1 M solution) to give [blog]409[/blog] and [blog]410[/blog] respectively.
Some precipitation was observed in the protein sample so it was spun down and returned to the sample cell.]]></content><html><![CDATA[<b>Project:</b> mthk<br />
To each of <a href="camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html">Octameric rck domain from MthK in D2O buffer</a> and <a href="camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html">Buffer background for octameric rck domain</a> (500 uL) in 1 mm SANS Tank cells was added CaCl2 (15 uL of a 1 M solution) to give <a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a> and <a href="camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html">Buffer background for octameric RCK plus Ca</a> respectively.<br style="clear:left;"/><br style="clear:left;"/>Some precipitation was observed in the protein sample so it was spun down and returned to the sample cell.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a>;<a href="camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html">Buffer background for octameric RCK plus Ca</a>;
]]></html><datestamp>2008-11-16T17:38:03+00:00</datestamp><timestamp>2008-11-16T18:09:23+00:00</timestamp><blog>5</blog><key>4c4aa4cb1d88ab8fc76d521b7a05611c</key><metadata><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a2</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.png</format></formats><comments/></post><post><id>417</id><rid>417</rid><title>Dimeric RCK from gel filtration in pD 6.5 D2O buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Dimeric RCK off gel filtration column in 20 mM MES 250 mM KCl, pD 6.5 in D2O
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
Dimeric RCK off gel filtration column in 20 mM MES 250 mM KCl, pD 6.5 in D2O<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/419/SANS_of_dimeric_RCK.html">SANS of dimeric RCK</a>;
]]></html><datestamp>2008-11-16T22:29:21+00:00</datestamp><timestamp>2008-11-16T22:29:21+00:00</timestamp><blog>5</blog><key>85d3f2c442509ac080bb968cc24b6614</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a6</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.png</format></formats><comments/></post><post><id>418</id><rid>418</rid><title>Buffer background for dimeric RCK</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[20 mM MES, 250 mM KCl, pD 6.5 in D2O
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
20 mM MES, 250 mM KCl, pD 6.5 in D2O<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/419/SANS_of_dimeric_RCK.html">SANS of dimeric RCK</a>;
]]></html><datestamp>2008-11-16T22:30:01+00:00</datestamp><timestamp>2008-11-16T22:30:01+00:00</timestamp><blog>5</blog><key>1d9b4e9ae5cc193c16ca5702f5135a17</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a7</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/418/Buffer_background_for_dimeric_RCK.png</format></formats><comments/></post><post><id>419</id><rid>421</rid><title>SANS of dimeric RCK</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][blog]417[/blog][col]T[col]10[col]46298[col][blog][/blog][/row]
[row][blog]418[/blog][col]T[col]10[col]46299[col][blog][/blog][/row]
[row][blog]417[/blog][col]S[col]85[col]46300[col][blog][/blog][/row]
[row][blog]418[/blog][col]S[col]85[col]46301[col][blog][/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.html">Dimeric RCK from gel filtration in pD 6.5 D2O buffer</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46298</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/418/Buffer_background_for_dimeric_RCK.html">Buffer background for dimeric RCK</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46299</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/417/Dimeric_RCK_from_gel_filtration_in_pD_65_D2O_buffer.html">Dimeric RCK from gel filtration in pD 6.5 D2O buffer</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46300</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/418/Buffer_background_for_dimeric_RCK.html">Buffer background for dimeric RCK</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46301</td><td class="table_st"></td></tr></table><br/>
]]></html><datestamp>2008-11-16T22:31:38+00:00</datestamp><timestamp>2008-11-16T22:33:43+00:00</timestamp><blog>5</blog><key>052747bd64b94dcab3e7e37e44bd36ba</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a8</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/419/SANS_of_dimeric_RCK.png</format></formats><comments/></post><post><id>412</id><rid>424</rid><title>SANS of octameric RCK plus Ca++</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][blog]409[/blog][col]T[col]10[col]46292[col][blog][/blog][/row]
[row][blog]410[/blog][col]T[col]10[col]46293[col][blog][/blog][/row]
[row][blog]409[/blog][col]S[col]85[col]46294[col][blog][/blog][/row]
[row][blog]410[/blog][col]S[col]85[col]46295[col][blog][/blog][/row]
[row][blog]409[/blog][col]S[col]85[col]46296[col][blog][/blog][/row]
[row][blog]410[/blog][col]S[col]85[col]46297[col][blog][/blog][/row]
[/table]
The order was changed after uploading the control script. The second control script is the correct one.]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46292</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html">Buffer background for octameric RCK plus Ca</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46293</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46294</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html">Buffer background for octameric RCK plus Ca</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46295</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46296</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/410/Buffer_background_for_octameric_RCK_plus_Ca.html">Buffer background for octameric RCK plus Ca</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46297</td><td class="table_st"></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>The order was changed after uploading the control script. The second control script is the correct one.<br/>
]]></html><datestamp>2008-11-16T17:58:16+00:00</datestamp><timestamp>2008-11-16T22:35:41+00:00</timestamp><blog>5</blog><key>c6b49643ecf5e75bb27a4ef4c1c0d087</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/88.xml</data><data>http://biolab.isis.rl.ac.uk/data/90.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/a5</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/412/SANS_of_octameric_RCK_plus_Ca.png</format></formats><comments/></post><post><id>426</id><rid>426</rid><title>Octameric RCK plus Ca dialysed into pD6.5</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Sample of octameric RCK with calcium added that was dialysed into pD 6.5 buffer in [blog]425[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
Sample of octameric RCK with calcium added that was dialysed into pD 6.5 buffer in <a href="camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html">Dialysis of Octameric RCK samples</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html">Dialysis of Octameric RCK samples</a>;<a href="camerons_labblog/434/SANS_on_LOQ_template.html">SANS on LOQ template</a>;
]]></html><datestamp>2008-11-17T01:00:49+00:00</datestamp><timestamp>2008-11-17T01:00:49+00:00</timestamp><blog>5</blog><key>8e7951e3dfe66b3a87d348555db48232</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/aa</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.png</format></formats><comments/></post><post><id>427</id><rid>430</rid><title>Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The sample of octameric RCK dialysed into 20 mM MES, 250 mM KCl pD 6.5]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
The sample of octameric RCK dialysed into 20 mM MES, 250 mM KCl pD 6.5<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html">Dialysis of Octameric RCK samples</a>;<a href="camerons_labblog/434/SANS_on_LOQ_template.html">SANS on LOQ template</a>;
]]></html><datestamp>2008-11-17T01:01:31+00:00</datestamp><timestamp>2008-11-17T01:04:25+00:00</timestamp><blog>5</blog><key>4e0f1a0bd9d97d5a37249bb6bf1f1e2b</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/ab</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.png</format></formats><comments/></post><post><id>425</id><rid>431</rid><title>Dialysis of Octameric RCK samples</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The samples [blog]387[/blog] and [blog]409[/blog] were dialysed into pD 6.5 buffer (MES, 250 mM KCl, one change) and the same buffer with 25 mM Ca2+ to give the samples [blog]427[/blog] and [blog]426[/blog] respectively.]]></content><html><![CDATA[<b>Project:</b> mthk<br />
The samples <a href="camerons_labblog/387/Octameric_rck_domain_from_MthK_in_D2O_buffer.html">Octameric rck domain from MthK in D2O buffer</a> and <a href="camerons_labblog/409/Octameric_RCK_plus_Ca.html">Octameric RCK plus Ca</a> were dialysed into pD 6.5 buffer (MES, 250 mM KCl, one change) and the same buffer with 25 mM Ca2+ to give the samples <a href="camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html">Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer</a> and <a href="camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html">Octameric RCK plus Ca dialysed into pD6.5</a> respectively.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html">Octameric RCK plus Ca dialysed into pD6.5</a>;
]]></html><datestamp>2008-11-17T01:00:35+00:00</datestamp><timestamp>2008-11-17T01:04:46+00:00</timestamp><blog>5</blog><key>977adc83a6cee25d669b90417564a96e</key><metadata><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/a9</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/425/Dialysis_of_Octameric_RCK_samples.png</format></formats><comments/></post><post><id>432</id><rid>432</rid><title>20 mM MES pD6.5 250 mM KCl in D2O</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Buffer pD 6.5 with no Ca2+
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
Buffer pD 6.5 with no Ca2+<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/434/SANS_on_LOQ_template.html">SANS on LOQ template</a>;
]]></html><datestamp>2008-11-17T01:05:19+00:00</datestamp><timestamp>2008-11-17T01:05:19+00:00</timestamp><blog>5</blog><key>e5eee28dd7870883785b8aad445c075d</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/ac</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.png</format></formats><comments/></post><post><id>433</id><rid>433</rid><title>20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2O</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Buffer pD 6.5 with Ca2+
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
Buffer pD 6.5 with Ca2+<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/434/SANS_on_LOQ_template.html">SANS on LOQ template</a>;
]]></html><datestamp>2008-11-17T01:05:45+00:00</datestamp><timestamp>2008-11-17T01:05:45+00:00</timestamp><blog>5</blog><key>09206e556e6792b92bdb7b6765a416d6</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/ad</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.png</format></formats><comments/></post><post><id>434</id><rid>438</rid><title>SANS of dialysed octameric RCK samples</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.
[table]
[row]Sample[col]SANS/TRANS[col]Exposure time (uamp.hr)[col]Run #[col]Data[/row]
[row][blog]427[/blog][col]T[col]10[col]46302[col][blog][/blog][/row]
[row][blog]432[/blog][col]T[col]10[col]46303[col][blog][/blog][/row]
[row][blog]426[/blog][col]T[col]10[col]46304[col][blog][/blog][/row]
[row][blog]433[/blog][col]T[col]10[col]46305[col][blog][/blog][/row]
[row][blog]427[/blog][col]S[col]85[col]46306[col][blog][/blog][/row]
[row][blog]432[/blog][col]S[col]85[col]46307[col][blog][/blog][/row]
[row][blog]426[/blog][col]S[col]85[col]46308[col][blog][/blog][/row]
[row][blog]433[/blog][col]S[col]85[col]46309[col][blog][/blog][/row]
[row][blog]427[/blog][col]S[col]85[col]46310[col][blog][/blog][/row]
[row][blog]432[/blog][col]S[col]85[col]46311[col][blog][/blog][/row]
[row][blog]426[/blog][col]S[col]85[col]46312[col][blog][/blog][/row]
[row][blog]433[/blog][col]S[col]85[col]46313[col][blog][/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SANS<br />
<b>Instrument:</b> LOQ<br />
The following samples were run as described on LOQ at ISIS. The samples were run in 1 mm path length tank cells (500 uL samples)l at a temperature of 16 C.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">SANS/TRANS</td><td class="table_st">Exposure time (uamp.hr)</td><td class="table_st">Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html">Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46302</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.html">20 mM MES pD6.5 250 mM KCl in D2O</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46303</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html">Octameric RCK plus Ca dialysed into pD6.5</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46304</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.html">20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2O</a></td><td class="table_st">T</td><td class="table_st">10</td><td class="table_st">46305</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html">Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46306</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.html">20 mM MES pD6.5 250 mM KCl in D2O</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46307</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html">Octameric RCK plus Ca dialysed into pD6.5</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46308</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.html">20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2O</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46309</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/427/Octameric_RCK_plus_no_Ca_dialysed_into_pD_65_buffer.html">Octameric RCK plus (no Ca) dialysed into pD 6.5 buffer</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46310</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/432/20_mM_MES_pD65_250_mM_KCl_in_D2O.html">20 mM MES pD6.5 250 mM KCl in D2O</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46311</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/426/Octameric_RCK_plus_Ca_dialysed_into_pD65.html">Octameric RCK plus Ca dialysed into pD6.5</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46312</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/433/20_mM_MES_pD65_250_mM_KCl_25_mM_Ca2_in_D2O.html">20 mM MES pD6.5 250 mM KCl, 25 mM Ca2+ in D2O</a></td><td class="table_st">S</td><td class="table_st">85</td><td class="table_st">46313</td><td class="table_st"></td></tr></table><br/>
]]></html><datestamp>2008-11-17T01:07:34+00:00</datestamp><timestamp>2008-11-17T01:14:29+00:00</timestamp><blog>5</blog><key>b4d0546b1766c45aea42e8762099d940</key><metadata><procedure>SANS</procedure><instrument>LOQ</instrument></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/92.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/ae</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/434/SANS_of_dialysed_octameric_RCK_samples.png</format></formats><comments/></post><post><id>374</id><rid>446</rid><title>Fresh beads for Laser Tweezers experiments</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[We have done a bunch of fairly random preliminary experiments that suggest that the beads made have very different stickiness. The [blog]300[/blog] seem to be pretty non-sticky but after protein loading they become much more sticky. We are going to prepare protein loaded beads on good quantities of beads to split into different buffers and wash extensively.
Sortase reaction mixture made from:
50 uL Sortase (8.4 uM)
50 uL CaCl2 (100 mM)
368 uL Tus (62 uM)
32 uL water
[Table]
[row]Beads[col]Wt of tube[col]Wt of tube + beads[col]uL Sortase reaction[col]Product[/row]
[row][blog]300[/blog][col]1.5482[col]1.5877[col]100[col][/row]
[row][blog]301[/blog][col]1.6434[col]1.6486[col]100[col][blog]445[/blog][/row]
[row][blog]302[/blog][col]1.6431[col]1.6473[col]100[col][blog]444[/blog][/row]
[row][blog]303[/blog][col]1.5906[col]1.5942[col]100[col][/row]
[/table]
The beads were incubated overnight with shaking at room temperature. In the morning they were washed twice with 5 mM Tris-HCl pH 7.5, 1 M NaCL (1 mL), then 1 x 1 mL of the same buffer with 400 mM NaCl, then 1 x 1 mL of buffer with 300 mM NaCl. Then 1 x 1 mL of 100 mM NaCl buffer.
After resuspension in 100 mM NaCl buffer the samples were then split into 4 x 250 uL. The 250 uL samples were spun and resuspended in 1 mL of 100 mM NaCl buffer, 50 mM NaCl buffer, 0 mM NaCl buffer and water and washed a further two times with this buffer.]]></content><html><![CDATA[<b>Project:</b> Laser_tweezers_tus<br />
We have done a bunch of fairly random preliminary experiments that suggest that the beads made have very different stickiness. The <a href="camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html">1:100 gly-oh beads after ammonia treatment</a> seem to be pretty non-sticky but after protein loading they become much more sticky. We are going to prepare protein loaded beads on good quantities of beads to split into different buffers and wash extensively.<br style="clear:left;"/><br style="clear:left;"/>Sortase reaction mixture made from:<br style="clear:left;"/>50 uL Sortase (8.4 uM)<br style="clear:left;"/>50 uL CaCl2 (100 mM)<br style="clear:left;"/>368 uL Tus (62 uM)<br style="clear:left;"/>32 uL water<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Beads</td><td class="table_st">Wt of tube</td><td class="table_st">Wt of tube + beads</td><td class="table_st">uL Sortase reaction</td><td class="table_st">Product</td></tr><tr><td class="table_st"><a href="camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html">1:100 gly-oh beads after ammonia treatment</a></td><td class="table_st">1.5482</td><td class="table_st">1.5877</td><td class="table_st">100</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/301/11000_glyOH_beads_after_ammonia_treatment.html">1:1000 gly-OH beads after ammonia treatment</a></td><td class="table_st">1.6434</td><td class="table_st">1.6486</td><td class="table_st">100</td><td class="table_st"><a href="shubbies_lablog/445/11000_Tus_loaded_silica_beads.html">1;1000 Tus loaded silica beads</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/302/110000_glyOH_beads_after_ammonia_treatment.html">1:10000 gly-OH beads after ammonia treatment</a></td><td class="table_st">1.6431</td><td class="table_st">1.6473</td><td class="table_st">100</td><td class="table_st"><a href="shubbies_lablog/444/New_110000_Tus_labelled_Silica_beads.html">New 1:10,000 Tus labelled Silica beads</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/303/1100000_glyOH_beads_after_ammonia_treatment.html">1:100,000 gly-OH beads after ammonia treatment</a></td><td class="table_st">1.5906</td><td class="table_st">1.5942</td><td class="table_st">100</td><td class="table_st"></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>The beads were incubated overnight with shaking at room temperature. In the morning they were washed twice with 5 mM Tris-HCl pH 7.5, 1 M NaCL (1 mL), then 1 x 1 mL of the same buffer with 400 mM NaCl, then 1 x 1 mL of buffer with 300 mM NaCl. Then 1 x 1 mL of 100 mM NaCl buffer.<br style="clear:left;"/><br style="clear:left;"/>After resuspension in 100 mM NaCl buffer the samples were then split into 4 x 250 uL. The 250 uL samples were spun and resuspended in 1 mL of 100 mM NaCl buffer, 50 mM NaCl buffer, 0 mM NaCl buffer and water and washed a further two times with this buffer.<br/><b>This Post is Linked By:</b> <a href="shubbies_lablog/444/New_110000_Tus_labelled_Silica_beads.html">New 1:10,000 Tus labelled Silica beads</a>;<a href="shubbies_lablog/445/11000_Tus_loaded_silica_beads.html">1;1000 Tus loaded silica beads</a>;
]]></html><datestamp>2008-11-13T17:52:44+00:00</datestamp><timestamp>2008-11-27T13:24:08+00:00</timestamp><blog>5</blog><key>5a4f761c532ac99880cabdb3a8a580a9</key><metadata><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/99</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/374/Fresh_beads_for_Laser_Tweezers_experiments.png</format></formats><comments/></post><post><id>454</id><rid>454</rid><title>DNA sequence data from Southampton on pLLC064</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Sequence data forwarded by guillame boucher from sequencing of the plasmid pLLC064 - supposedly Sortase in pETMCSI
TTTTGTTTACTTTAAGAAGGAGATATACATATGAAACCACATATCGATAATTATCTTCAC
GATAAAGATAAAGATGAAAAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGT
AAAGATAAAAAGCAGCAAGCTAAACCTCAAATTCCGAAAGATAAATCGAAAGTGGCAGGC
TATATTGAAATTCCAGATGCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCT
GAACAATTAAATAGAGGTGTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAAT
ATTTCAATTGCAGGACACACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAA
GCAGCCAAAAAAGGTAGTATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAA
ATGACAAGTATAAGAGATGTTAAGCCTACAGATGTAGGAGTTCTAGATGAACAAAAAGGT
AAAGATAAACAATTAACATTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGG
GAAAAACGTAAAATCTTTGTAGCTACAGAAGTCAAATAATCGAATTCGAGCTCCCGGGTA
CCATGGCATGCATCGATAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT
GCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGG
GGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCACAGGACGGGTGTGGTCGCCAT
GATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTGGGCGGCGGCCAAA
GCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAAATTGCATCAACGCATATAGCGC
TAGCAGCACGCCATAGTGACTGGCCGATGCTGTCGGAATGGACGATATCCCGCAAGAGGC
CCGGCAGTACCGGCATAACCAAGCCTATGCCT]]></content><html><![CDATA[<b>Data Type:</b> Sequence_DNA<br />
Sequence data forwarded by guillame boucher from sequencing of the plasmid pLLC064 - supposedly Sortase in pETMCSI<br style="clear:left;"/><br style="clear:left;"/>TTTTGTTTACTTTAAGAAGGAGATATACATATGAAACCACATATCGATAATTATCTTCAC<br style="clear:left;"/>GATAAAGATAAAGATGAAAAGATTGAACAATATGATAAAAATGTAAAAGAACAGGCGAGT<br style="clear:left;"/>AAAGATAAAAAGCAGCAAGCTAAACCTCAAATTCCGAAAGATAAATCGAAAGTGGCAGGC<br style="clear:left;"/>TATATTGAAATTCCAGATGCTGATATTAAAGAACCAGTATATCCAGGACCAGCAACACCT<br style="clear:left;"/>GAACAATTAAATAGAGGTGTAAGCTTTGCAGAAGAAAATGAATCACTAGATGATCAAAAT<br style="clear:left;"/>ATTTCAATTGCAGGACACACTTTCATTGACCGTCCGAACTATCAATTTACAAATCTTAAA<br style="clear:left;"/>GCAGCCAAAAAAGGTAGTATGGTGTACTTTAAAGTTGGTAATGAAACACGTAAGTATAAA<br style="clear:left;"/>ATGACAAGTATAAGAGATGTTAAGCCTACAGATGTAGGAGTTCTAGATGAACAAAAAGGT<br style="clear:left;"/>AAAGATAAACAATTAACATTAATTACTTGTGATGATTACAATGAAAAGACAGGCGTTTGG<br style="clear:left;"/>GAAAAACGTAAAATCTTTGTAGCTACAGAAGTCAAATAATCGAATTCGAGCTCCCGGGTA<br style="clear:left;"/>CCATGGCATGCATCGATAGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCT<br style="clear:left;"/>GCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGG<br style="clear:left;"/>GGTTTTTTGCTGAAAGGAGGAACTATATCCGGATATCCACAGGACGGGTGTGGTCGCCAT<br style="clear:left;"/>GATCGCGTAGTCGATAGTGGCTCCAAGTAGCGAAGCGAGCAGGACTGGGCGGCGGCCAAA<br style="clear:left;"/>GCGGTCGGACAGTGCTCCGAGAACGGGTGCGCATAGAAATTGCATCAACGCATATAGCGC<br style="clear:left;"/>TAGCAGCACGCCATAGTGACTGGCCGATGCTGTCGGAATGGACGATATCCCGCAAGAGGC<br style="clear:left;"/>CCGGCAGTACCGGCATAACCAAGCCTATGCCT<br/>
]]></html><datestamp>2008-11-28T11:02:19+00:00</datestamp><timestamp>2008-11-28T11:02:19+00:00</timestamp><blog>5</blog><key>1d219eabdb43425aad7b512a551f1ab2</key><metadata><data_type>Sequence_DNA</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/b7</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/454/DNA_sequence_data_from_Southampton_on_pLLC064.png</format></formats><comments/></post><post><id>474</id><rid>476</rid><title>Analysis of pLLC064 sequencing data</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Looking at the sequence provided by Guillame and comparing it to the sequences of pETMCSI and pETMCSIII it is evident that this plasmid is the original sequence that Krystle cloned into pETMCSI in 2004 rather than the version is pETMCSIII. This explains why in previous work the protein didn't bind to the nickel column but was apparently well expressed. My guess is that Lilyan mis-labelled the plasmids when they were placed in the storage box. It also means that the plasmid I have sent out to various people isn't what I said it was.
The good news is that Ali Tavasolli's group at Soton are going to re-clone "our" Sortase gene and we can then re-make the properly designated pLLC064.]]></content><html><![CDATA[<b>Procedure:</b> Data_analysis<br />
Looking at the sequence provided by Guillame and comparing it to the sequences of pETMCSI and pETMCSIII it is evident that this plasmid is the original sequence that Krystle cloned into pETMCSI in 2004 rather than the version is pETMCSIII. This explains why in previous work the protein didn't bind to the nickel column but was apparently well expressed. My guess is that Lilyan mis-labelled the plasmids when they were placed in the storage box. It also means that the plasmid I have sent out to various people isn't what I said it was.<br style="clear:left;"/><br style="clear:left;"/>The good news is that Ali Tavasolli's group at Soton are going to re-clone "our" Sortase gene and we can then re-make the properly designated pLLC064.<br/>
]]></html><datestamp>2008-12-01T11:16:32+00:00</datestamp><timestamp>2008-12-01T11:19:11+00:00</timestamp><blog>5</blog><key>1a8ea5e0a6017a464a35d3b7b61f9639</key><metadata><procedure>Data_analysis</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/c1</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/474/Analysis_of_pLLC064_sequencing_data.png</format></formats><comments/></post><post><id>766</id><rid>766</rid><title>PCR template</title><section>Templates</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[PCR reactions were run using the following sample conditions. The PCR cycling programme was [[blog]].
[table]
[row]
Reaction[col]Template[col]uL[col]Primer 1[col]ul[col]Primer 2[col]uL[col]Buffer[col]uL[col]Enzyme[col]uL[col]dNTP[col]ul[col]Mg[col]uL[col]Water (uL)[col]Product
[/row]
[row]
1[col][[Dna:%]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]]
[col][[box]][col][[box=3]][col][[box]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]]
[col][[Dna:pcr_product]][/row]
[row]
2[col][[Dna:%]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]]
[col][[box]][col][[box=3]][col][[box]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]]
[col][[Dna:pcr_product]][/row]
[row]
3[col][[Dna:%]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]]
[col][[box]][col][[box=3]][col][[box]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]]
[col][[Dna:pcr_product]][/row]
[row]
4[col][[Dna:%]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]]
[col][[box]][col][[box=3]][col][[box]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]]
[col][[Dna:pcr_product]][/row]
[row]
5[col][[Dna:%]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]][col][[Dna:oligonucleotide]][col][[box=3]]
[col][[box]][col][[box=3]][col][[box]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]][col][[box=3]]
[col][[Dna:pcr_product]][/row]
[/table]
[[Section>Procedure]]
[[Procedure>PCR]]]]></content><html><![CDATA[PCR reactions were run using the following sample conditions. The PCR cycling programme was [[blog]].<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st"><br style="clear:left;"/>Reaction</td><td class="table_st">Template</td><td class="table_st">uL</td><td class="table_st">Primer 1</td><td class="table_st">ul</td><td class="table_st">Primer 2</td><td class="table_st">uL</td><td class="table_st">Buffer</td><td class="table_st">uL</td><td class="table_st">Enzyme</td><td class="table_st">uL</td><td class="table_st">dNTP</td><td class="table_st">ul</td><td class="table_st">Mg</td><td class="table_st">uL</td><td class="table_st">Water (uL)</td><td class="table_st">Product<br style="clear:left;"/></td></tr><tr><td class="table_st"><br style="clear:left;"/>1</td><td class="table_st">[[Dna:%]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[Dna:pcr_product]]</td></tr><tr><td class="table_st"><br style="clear:left;"/>2</td><td class="table_st">[[Dna:%]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[Dna:pcr_product]]</td></tr><tr><td class="table_st"><br style="clear:left;"/>3</td><td class="table_st">[[Dna:%]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[Dna:pcr_product]]</td></tr><tr><td class="table_st"><br style="clear:left;"/>4</td><td class="table_st">[[Dna:%]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[Dna:pcr_product]]</td></tr><tr><td class="table_st"><br style="clear:left;"/>5</td><td class="table_st">[[Dna:%]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[Dna:oligonucleotide]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]</td><td class="table_st">[[box=3]]<br style="clear:left;"/></td><td class="table_st">[[Dna:pcr_product]]</td></tr></table><br style="clear:left;"/><br style="clear:left;"/>[[Section>Procedure]]<br style="clear:left;"/>[[Procedure>PCR]]<br/>
]]></html><datestamp>2008-12-09T10:36:01+00:00</datestamp><timestamp>2008-12-09T10:36:01+00:00</timestamp><blog>5</blog><key>d4e046ea08b8353327e4e4349245018f</key><links><uri>http://biolab.isis.rl.ac.uk/uri/110</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/766/PCR_template.png</format></formats><comments/></post><post><id>390</id><rid>815</rid><title>Buffer background for octameric rck domain</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[20 mM Tris-HCl pD 8.0, 250 mM KCl]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> mthk<br />
20 mM Tris-HCl pD 8.0, 250 mM KCl<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/402/SANS_runs_on_octameric_RCK.html">SANS runs on octameric RCK</a>;<a href="camerons_labblog/405/Additional_runs_on_octameric_Rck.html">Additional runs on octameric Rck</a>;<a href="camerons_labblog/408/Addition_of_Ca_to_octameric_mthk_sample.html">Addition of Ca++ to octameric mthk sample</a>;
]]></html><datestamp>2008-11-16T11:23:39+00:00</datestamp><timestamp>2008-12-10T13:51:32+00:00</timestamp><blog>5</blog><key>2caddbf4274f8312722a7214fcb761b2</key><metadata><material>Solution</material><project>mthk</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/9e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/390/Buffer_background_for_octameric_rck_domain.png</format></formats><comments/></post><post><id>765</id><rid>816</rid><title>KcsA plasmid from Southampton</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[A pET based plasmid containing the KcsA gene, a few micrograms resuspended in 20 uL of nuclease free water.]]></content><html><![CDATA[<b>Dna:</b> plasmid<br />
<b>Material:</b> Solution<br />
A pET based plasmid containing the KcsA gene, a few micrograms resuspended in 20 uL of nuclease free water.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.html">Test PCR to insert Sortase sequence within KcsA</a>;
]]></html><datestamp>2008-12-09T10:34:18+00:00</datestamp><timestamp>2008-12-10T13:54:42+00:00</timestamp><blog>5</blog><key>f1474f2acae2bd3c829a5d5b2dddbb0a</key><metadata><dna>plasmid</dna><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/10f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/765/KcsA_plasmid_from_Southampton.png</format></formats><comments/></post><post><id>757</id><rid>817</rid><title>kcsa-sort-bwd</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]kcsa-sort-bwd[/row]
[row]Number[col][/row]
[row]Location[col][/row]
[row]Sequence[col]CTGCCGGGTACCGGTGGTGCGCAGCTGATCACGTATCCGC[/row]
[row]Length[col]40[/row]
[row]Melting temp[col]71 (64) C[/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col]100 uM[/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">kcsa-sort-bwd</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st"></td></tr><tr><td class="table_st">Sequence</td><td class="table_st">CTGCCGGGTACCGGTGGTGCGCAGCTGATCACGTATCCGC</td></tr><tr><td class="table_st">Length</td><td class="table_st">40</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st">71 (64) C</td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st">100 uM</td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.html">Test PCR to insert Sortase sequence within KcsA</a>;
]]></html><datestamp>2008-12-09T10:24:45+00:00</datestamp><timestamp>2008-12-10T13:55:44+00:00</timestamp><blog>5</blog><key>ff7f3737c4f62cda5789e430513d3aff</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/116.xml</data><data>http://biolab.isis.rl.ac.uk/data/118.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/10e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/757/kcsasortbwd.png</format></formats><comments/></post><post><id>756</id><rid>818</rid><title>kcsa-sort-fwd</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table][mrow]Property[mcol]Data[/mrow]
[row]Name[col]kcsa-sort-fwd[/row]
[row]Number[col][/row]
[row]Location[col][/row]
[row]Sequence[col]CGCACCACCGGTACCGGCAGACCTGCGCCGCCTCAGCCAG[/row]
[row]Length[col]40[/row]
[row]Melting temp[col]74 (64) C[/row]
[row]Supplier[col]Invitrogen[/row]
[row]Stock concentration[col]100 uM[/row][/table]]]></content><html><![CDATA[<b>Dna:</b> oligonucleotide<br />
<b>Material:</b> Solution<br />
<table class="table_st" cellspacing="0"><tr class="table_title"><td class="table_st">Property</td><td class="table_st">Data</td></tr><tr><td class="table_st">Name</td><td class="table_st">kcsa-sort-fwd</td></tr><tr><td class="table_st">Number</td><td class="table_st"></td></tr><tr><td class="table_st">Location</td><td class="table_st"></td></tr><tr><td class="table_st">Sequence</td><td class="table_st">CGCACCACCGGTACCGGCAGACCTGCGCCGCCTCAGCCAG</td></tr><tr><td class="table_st">Length</td><td class="table_st">40</td></tr><tr><td class="table_st">Melting temp</td><td class="table_st">74 (64) C</td></tr><tr><td class="table_st">Supplier</td><td class="table_st">Invitrogen</td></tr><tr><td class="table_st">Stock concentration</td><td class="table_st">100 uM</td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.html">Test PCR to insert Sortase sequence within KcsA</a>;
]]></html><datestamp>2008-12-09T10:23:18+00:00</datestamp><timestamp>2008-12-10T13:55:59+00:00</timestamp><blog>5</blog><key>20ac5712f22bb4333175022ae283d31e</key><metadata><dna>oligonucleotide</dna><material>Solution</material></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/120.xml</data><data>http://biolab.isis.rl.ac.uk/data/122.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/10d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/756/kcsasortfwd.png</format></formats><comments/></post><post><id>769</id><rid>820</rid><title>Test PCR to insert Sortase sequence within KcsA</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Two large PCR reactions masters were set up as described in the table.
[table]
[row]
Reaction[col]Template[col]uL[col]Primer 1[col]ul[col]Primer 2[col]uL[col]Buffer[col]uL[col]Enzyme[col]uL[col]dNTP[col]ul[col]Mg[col]uL[col]Water (uL)[col]Product
[/row]
[row]
1[col][blog]765[/blog][col]5[col][blog]756[/blog][col]1[col][blog]757[/blog][col]1
[col]Thermopol buffer[col]10[col]Vent[col]1[col]2 mM[col]10[col]100 mM[col]0[col]72
[/row]
[row]
2[col][blog]765[/blog][col]5[col][blog]756[/blog][col]1[col][blog]757[/blog][col]1
[col]Thermopol buffer[col]10[col]Vent[col]1[col]2 mM[col]10[col]100 mM[col]1[col]71[/row]
[/table]
Each of the two was then divided into ten PCR tubes and spread across the plate of the thermal cycler to have a gradient on the annealing phase of the PCR reaction from 40-60 C.
Samples of each rxn (5 uL) were run on an agarose gel and stained. Not a thing on it! Which is odd given the amount of MgSO4 in the second set of reactions. Will have to try again...]]></content><html><![CDATA[<b>Procedure:</b> PCR<br />
<b>Project:</b> kcsa<br />
Two large PCR reactions masters were set up as described in the table.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st"><br style="clear:left;"/>Reaction</td><td class="table_st">Template</td><td class="table_st">uL</td><td class="table_st">Primer 1</td><td class="table_st">ul</td><td class="table_st">Primer 2</td><td class="table_st">uL</td><td class="table_st">Buffer</td><td class="table_st">uL</td><td class="table_st">Enzyme</td><td class="table_st">uL</td><td class="table_st">dNTP</td><td class="table_st">ul</td><td class="table_st">Mg</td><td class="table_st">uL</td><td class="table_st">Water (uL)</td><td class="table_st">Product<br style="clear:left;"/></td></tr><tr><td class="table_st"><br style="clear:left;"/>1</td><td class="table_st"><a href="camerons_labblog/765/KcsA_plasmid_from_Southampton.html">KcsA plasmid from Southampton</a></td><td class="table_st">5</td><td class="table_st"><a href="camerons_labblog/756/kcsasortfwd.html">kcsa-sort-fwd</a></td><td class="table_st">1</td><td class="table_st"><a href="camerons_labblog/757/kcsasortbwd.html">kcsa-sort-bwd</a></td><td class="table_st">1<br style="clear:left;"/></td><td class="table_st">Thermopol buffer</td><td class="table_st">10</td><td class="table_st">Vent</td><td class="table_st">1</td><td class="table_st">2 mM</td><td class="table_st">10</td><td class="table_st">100 mM</td><td class="table_st">0</td><td class="table_st">72<br style="clear:left;"/></td></tr><tr><td class="table_st"><br style="clear:left;"/>2</td><td class="table_st"><a href="camerons_labblog/765/KcsA_plasmid_from_Southampton.html">KcsA plasmid from Southampton</a></td><td class="table_st">5</td><td class="table_st"><a href="camerons_labblog/756/kcsasortfwd.html">kcsa-sort-fwd</a></td><td class="table_st">1</td><td class="table_st"><a href="camerons_labblog/757/kcsasortbwd.html">kcsa-sort-bwd</a></td><td class="table_st">1<br style="clear:left;"/></td><td class="table_st">Thermopol buffer</td><td class="table_st">10</td><td class="table_st">Vent</td><td class="table_st">1</td><td class="table_st">2 mM</td><td class="table_st">10</td><td class="table_st">100 mM</td><td class="table_st">1</td><td class="table_st">71</td></tr></table><br style="clear:left;"/><br style="clear:left;"/>Each of the two was then divided into ten PCR tubes and spread across the plate of the thermal cycler to have a gradient on the annealing phase of the PCR reaction from 40-60 C.<br style="clear:left;"/><br style="clear:left;"/>Samples of each rxn (5 uL) were run on an agarose gel and stained. Not a thing on it! Which is odd given the amount of MgSO4 in the second set of reactions. Will have to try again...<br/>
]]></html><datestamp>2008-12-09T10:47:35+00:00</datestamp><timestamp>2008-12-10T14:16:59+00:00</timestamp><blog>5</blog><key>58aa3a920bb1641d5a80e924993eda5d</key><metadata><procedure>PCR</procedure><project>kcsa</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/124.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/112</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/769/Test_PCR_to_insert_Sortase_sequence_within_KcsA.png</format></formats><comments/></post><post><id>353</id><rid>2261</rid><title>First preparation of beads for laser tweezers experiment</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approximately 1 mg of [blog]300[/blog] was taken for resuspension in Sortase labelling reaction. [blog]263[/blog] (1uL) was added to [blog]352[/blog] (10uL) and allowed to incubate for ~15 minutes after which 1 mL of water was added and the beads spun, supernatant discarded and the beads resuspended in 1mL of water.]]></content><html><![CDATA[<b>Project:</b> Laser_tweezers_tus<br />
Approximately 1 mg of <a href="camerons_labblog/300/1100_glyoh_beads_after_ammonia_treatment.html">1:100 gly-oh beads after ammonia treatment</a> was taken for resuspension in Sortase labelling reaction. <a href="camerons_labblog/263/Phosphorylated_TerR_lam.html">Phosphorylated TerR lam</a> (1uL) was added to <a href="camerons_labblog/352/Bangs_Labs_streptavidin_coated_beads.html">Bangs Labs streptavidin coated beads</a> (10uL) and allowed to incubate for ~15 minutes after which 1 mL of water was added and the beads spun, supernatant discarded and the beads resuspended in 1mL of water.<br/>
]]></html><datestamp>2008-11-12T14:12:44+00:00</datestamp><timestamp>2009-01-23T15:03:27+00:00</timestamp><blog>5</blog><key>9f8189d2d6d1e3421fb30f3166051a68</key><metadata><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/90</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/353/First_preparation_of_beads_for_laser_tweezers_experiment.png</format></formats><comments/></post><post><id>261</id><rid>2262</rid><title>Phosphorylation of DNA substrates for laser tweezers experiment</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]DNA substrate[col]volume[col][blog]259[/blog][col][blog]260[/blog][col]ATP (10 mM)[col]Water[col]Product[/row]
[row][blog]240[/blog][col]200[col]1[col]50[col]25[col]0[col][blog]262[/blog][/row]
[row][blog]224[/blog][col]10[col]1[col]5[col]2.5[col]7.5[col][blog]269[/blog][/row]
[row][blog]221[/blog][col]10[col]1[col]5[col]2.5[col]7.5[col][blog]263[/blog][/row]
[row][blog]218[/blog][col]10[col]1[col]5[col]2.5[col]7.5[col][blog]264[/blog][/row]
[row][blog]214[/blog][col]10[col]1[col]5[col]2.5[col]7.5[col][blog]265[/blog][/row]
[row][blog]211[/blog][col]10[col]1[col]5[col]2.5[col]7.5[col][blog]266[/blog][/row]
[/table]
Samples were prepared as given and incubated at 37 C for 10' followed by 10' at 65 C to inactivate the enzyme.]]></content><html><![CDATA[<b>Procedure:</b> DNA_phosphorylation<br />
<b>Project:</b> Laser_tweezers_tus<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">DNA substrate</td><td class="table_st">volume</td><td class="table_st"><a href="lab_materials/259/T4_Polynucleotide_Kinase.html">T4 Polynucleotide Kinase</a></td><td class="table_st"><a href="lab_materials/260/5_x_Kinase_Forward_reaction_buffer.html">5 x Kinase Forward reaction buffer</a></td><td class="table_st">ATP (10 mM)</td><td class="table_st">Water</td><td class="table_st">Product</td></tr><tr><td class="table_st"><a href="camerons_labblog/240/Ethanol_precipitated_lambda_DNA.html">Ethanol precipitated lambda DNA</a></td><td class="table_st">200</td><td class="table_st">1</td><td class="table_st">50</td><td class="table_st">25</td><td class="table_st">0</td><td class="table_st"><a href="camerons_labblog/262/Phosphorylated_lambda_DNA.html">Phosphorylated lambda DNA</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/224/Oligolambdabiotin.html">Oligo-lambda-biotin</a></td><td class="table_st">10</td><td class="table_st">1</td><td class="table_st">5</td><td class="table_st">2.5</td><td class="table_st">7.5</td><td class="table_st"><a href="camerons_labblog/269/Phosphorylated_oligolambdabiotin.html">Phosphorylated oligo-lambda-biotin</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/221/OligoTerRlam.html">Oligo-TerRlam</a></td><td class="table_st">10</td><td class="table_st">1</td><td class="table_st">5</td><td class="table_st">2.5</td><td class="table_st">7.5</td><td class="table_st"><a href="camerons_labblog/263/Phosphorylated_TerR_lam.html">Phosphorylated TerR lam</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/218/OligoTerR.html">Oligo-TerR</a></td><td class="table_st">10</td><td class="table_st">1</td><td class="table_st">5</td><td class="table_st">2.5</td><td class="table_st">7.5</td><td class="table_st"><a href="camerons_labblog/264/Phosporylated_TerR.html">Phosporylated TerR</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/214/OligoTerFlam.html">Oligo-TerFlam</a></td><td class="table_st">10</td><td class="table_st">1</td><td class="table_st">5</td><td class="table_st">2.5</td><td class="table_st">7.5</td><td class="table_st"><a href="camerons_labblog/265/Phosphorylated_TerRlam.html">Phosphorylated TerRlam</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/211/OligoTerF.html">Oligo-TerF</a></td><td class="table_st">10</td><td class="table_st">1</td><td class="table_st">5</td><td class="table_st">2.5</td><td class="table_st">7.5</td><td class="table_st"><a href="camerons_labblog/266/Phosphorylated_TerR.html">Phosphorylated TerR</a></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>Samples were prepared as given and incubated at 37 C for 10' followed by 10' at 65 C to inactivate the enzyme.<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/263/Phosphorylated_TerR_lam.html">Phosphorylated TerR lam</a>;<a href="camerons_labblog/264/Phosporylated_TerR.html">Phosporylated TerR</a>;<a href="camerons_labblog/265/Phosphorylated_TerRlam.html">Phosphorylated TerRlam</a>;<a href="camerons_labblog/269/Phosphorylated_oligolambdabiotin.html">Phosphorylated oligo-lambda-biotin</a>;<a href="camerons_labblog/262/Phosphorylated_lambda_DNA.html">Phosphorylated lambda DNA</a>;<a href="camerons_labblog/266/Phosphorylated_TerR.html">Phosphorylated TerR</a>;
]]></html><datestamp>2008-11-05T15:59:58+00:00</datestamp><timestamp>2009-01-23T15:03:53+00:00</timestamp><blog>5</blog><key>e9a9f853ecf07850581e7ab794784a6c</key><metadata><procedure>DNA_phosphorylation</procedure><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/65</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/261/Phosphorylation_of_DNA_substrates_for_laser_tweezers_experiment.png</format></formats><comments/></post><post><id>239</id><rid>2263</rid><title>Ethanol precipitation of lambda DNA</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Because the [blog]228[/blog] is in a tris-edta buffer I will ethanol ppt it and resuspend in water.
200 uL of [blog]228[/blog] was added to 20 uL of 3 M ammonium acetate pH 4.8 followed by 400 uL of ethanol. The solution was mixed and incubated on ice for ~60 minutes. The solution was then centrifuged at 20k x g for 20 minutes.
The DNA was then further dried overnight and resuspended in nuclease free water to give [blog]240[/blog]]]></content><html><![CDATA[<b>Project:</b> Laser_tweezers_tus<br />
Because the <a href="lab_materials/228/Lambda_DNA.html">Lambda DNA</a> is in a tris-edta buffer I will ethanol ppt it and resuspend in water. <br style="clear:left;"/><br style="clear:left;"/>200 uL of <a href="lab_materials/228/Lambda_DNA.html">Lambda DNA</a> was added to 20 uL of 3 M ammonium acetate pH 4.8 followed by 400 uL of ethanol. The solution was mixed and incubated on ice for ~60 minutes. The solution was then centrifuged at 20k x g for 20 minutes.<br style="clear:left;"/><br style="clear:left;"/>The DNA was then further dried overnight and resuspended in nuclease free water to give <a href="camerons_labblog/240/Ethanol_precipitated_lambda_DNA.html">Ethanol precipitated lambda DNA</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/240/Ethanol_precipitated_lambda_DNA.html">Ethanol precipitated lambda DNA</a>;
]]></html><datestamp>2008-10-28T15:35:06+00:00</datestamp><timestamp>2009-01-23T15:04:27+00:00</timestamp><blog>5</blog><key>d4a62a6806f5e7bb7c0317df6f16bd3e</key><metadata><project>Laser_tweezers_tus</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/5b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/239/Ethanol_precipitation_of_lambda_DNA.png</format></formats><comments/></post><post><id>2868</id><rid>3262</rid><title>Further lysozyme runs</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate. 0.5 mm of Al was used to attenuate the beam from this point on.
[table]
[row]Sample[col]Frames[col]Exposure time [col]I22 Raw Run #[col]Data[/row]
[row][blog]2790[/blog][col]30[col]30[col]A10000.202[col][blog]2838[/blog][/row]
[row][blog]2778[/blog][col]30[col]30[col]A11000.202[col][blog]2837[/blog][/row]
[row][blog]2788[/blog][col]30[col]30[col]A12000.2020[col][blog]2858[/blog][/row]
[row][blog]2788[/blog][col]10[col]30[col]A13000.202[col][blog]2859[/blog][/row]
[row][blog]2787[/blog][col]30[col]30[col]A14000.202[col][blog]2860[/blog][/row]
[row][blog]2786[/blog][col]30[col]30[col]A15000.202[col][blog]2863[/blog][/row]
[row][blog]2782[/blog][col]30[col]30[col]A16000.202[col][blog]2864[/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SAXS<br />
<b>Instrument:</b> I22<br />
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate. 0.5 mm of Al was used to attenuate the beam from this point on.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Frames</td><td class="table_st">Exposure time </td><td class="table_st">I22 Raw Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/2790/Lysozyme_10_mgmL.html">Lysozyme (~10 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A10000.202</td><td class="table_st"><a href="camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.html">Lysozyme 10 mg/mL with attenutor</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2778/Lysozyme_20_mgmL.html">Lysozyme (~20 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A11000.202</td><td class="table_st"><a href="camerons_labblog/2837/Lysozyme_20_mgmL_wo_attenutor.html">Lysozyme 20 mg/mL w/o attenutor</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2788/GFP_2_mgmL.html">GFP (~2 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A12000.2020</td><td class="table_st"><a href="camerons_labblog/2858/GFP_2mgmL.html">GFP 2mg/mL</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2788/GFP_2_mgmL.html">GFP (~2 mg/mL)</a></td><td class="table_st">10</td><td class="table_st">30</td><td class="table_st">A13000.202</td><td class="table_st"><a href="camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.html">second 10 frames of 2 mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2787/GFP_5_mgmL.html">GFP (~5 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A14000.202</td><td class="table_st"><a href="camerons_labblog/2860/GFP_5_mgmL.html">GFP 5 mg/mL</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2786/GFP_10mgmL.html">GFP (~10mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A15000.202</td><td class="table_st"><a href="camerons_labblog/2863/GFP_10mgmL.html">GFP 10mg/mL</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2782/GFP_20_mgmL.html">GFP (~20 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A16000.202</td><td class="table_st"><a href="camerons_labblog/2864/GFP_20mgmL.html">GFP 20mg/mL</a></td></tr></table><br/>
]]></html><datestamp>2009-02-02T19:25:10+00:00</datestamp><timestamp>2009-02-10T11:36:21+00:00</timestamp><blog>5</blog><key>91ffa4469e7428b450cc167ead31edf0</key><metadata><procedure>SAXS</procedure><instrument>I22</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/16d</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2868/Further_lysozyme_runs.png</format></formats><comments/></post><post><id>3226</id><rid>3228</rid><title>UV-Vis of 5mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From[blog]3219[/blog][data]382[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From<a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=382','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=382&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:33:38+00:00</datestamp><timestamp>2009-02-09T15:33:54+00:00</timestamp><blog>5</blog><key>88ab82378cc2663c932d9ccbc0b06b3f</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/382.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1a9</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.png</format></formats><comments/></post><post><id>3229</id><rid>3229</rid><title>UV-Vis of 2mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:34:28+00:00</datestamp><timestamp>2009-02-09T15:34:28+00:00</timestamp><blog>5</blog><key>a156ec6c8397903f734509413c2cba95</key><metadata><data_type>UV-Vis</data_type></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/1aa</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.png</format></formats><comments/></post><post><id>3230</id><rid>3232</rid><title>UV-Vis of 20mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]384[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=384','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=384&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:34:55+00:00</datestamp><timestamp>2009-02-09T15:35:11+00:00</timestamp><blog>5</blog><key>91b41b2b6d3c8ee782f51c8b659ec0ba</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/384.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1ab</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3230/UVVis_of_20mgmL_GFP.png</format></formats><comments/></post><post><id>3233</id><rid>3235</rid><title>UV-Vis of 10mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]386[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=386','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=386&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:35:35+00:00</datestamp><timestamp>2009-02-09T15:36:03+00:00</timestamp><blog>5</blog><key>fe7d37bb8a4bd18e0ef4b62ec265928a</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/386.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1ac</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3233/UVVis_of_10mgmL_GFP.png</format></formats><comments/></post><post><id>3236</id><rid>3238</rid><title>UV-Vis of 5mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]388[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=388','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=388&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:36:27+00:00</datestamp><timestamp>2009-02-09T15:36:45+00:00</timestamp><blog>5</blog><key>b1a9b6feb83441f5a2d8adc87fd2a569</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/388.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1ad</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3236/UVVis_of_5mgmL_GFP.png</format></formats><comments/></post><post><id>3239</id><rid>3241</rid><title>UV-Vis of 2mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]390[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=390','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=390&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:37:09+00:00</datestamp><timestamp>2009-02-09T15:37:24+00:00</timestamp><blog>5</blog><key>cb7457c3feec927f51ee2b6380cdadf1</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/390.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1ae</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3239/UVVis_of_2mgmL_GFP.png</format></formats><comments/></post><post><id>3219</id><rid>3242</rid><title>UV-Vis of Lysozyme and GFP samples from I22 SAXS</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Samples (10 uL) were run in a 5mm path length microcell on the GeneQuant 1300. The instrument was zeroed with a water background.
[table]
[row]Sample[col]Dilution[col]Data[/row]
[row][blog]2778[/blog][col]20-fold[col][blog]3220[/blog][/row]
[row][blog]2790[/blog][col]20-fold[col][blog]3223[/blog][/row]
[row][blog]2791[/blog][col]10-fold[col][blog]3226[/blog][/row]
[row][blog]2792[/blog][col]5-fold[col][blog]3229[/blog][/row]
[row][blog]2782[/blog][col]10-fold[col][blog]3230[/blog][/row]
[row][blog]2786[/blog][col]2-fold[col][blog]3233[/blog][/row]
[row][blog]2787[/blog][col]2-fold[col][blog]3236[/blog][/row]
[row][blog]2788[/blog][col]N/a[col][blog]3239[/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> UV-Vis<br />
Samples (10 uL) were run in a 5mm path length microcell on the GeneQuant 1300. The instrument was zeroed with a water background.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Dilution</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/2778/Lysozyme_20_mgmL.html">Lysozyme (~20 mg/mL)</a></td><td class="table_st">20-fold</td><td class="table_st"><a href="camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.html">UV-Vis of 20mg/mL Lysozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2790/Lysozyme_10_mgmL.html">Lysozyme (~10 mg/mL)</a></td><td class="table_st">20-fold</td><td class="table_st"><a href="camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.html">UV-Vis of 10mg/mL Lysozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2791/Lysozyme_5_mgmL.html">Lysozyme (~5 mg/mL)</a></td><td class="table_st">10-fold</td><td class="table_st"><a href="camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.html">UV-Vis of 5mg/mL Lysozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2792/Lysozyme_2_mgmL.html">Lysozyme (~2 mg/mL)</a></td><td class="table_st">5-fold</td><td class="table_st"><a href="camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.html">UV-Vis of 2mg/mL Lysozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2782/GFP_20_mgmL.html">GFP (~20 mg/mL)</a></td><td class="table_st">10-fold</td><td class="table_st"><a href="camerons_labblog/3230/UVVis_of_20mgmL_GFP.html">UV-Vis of 20mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2786/GFP_10mgmL.html">GFP (~10mg/mL)</a></td><td class="table_st">2-fold</td><td class="table_st"><a href="camerons_labblog/3233/UVVis_of_10mgmL_GFP.html">UV-Vis of 10mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2787/GFP_5_mgmL.html">GFP (~5 mg/mL)</a></td><td class="table_st">2-fold</td><td class="table_st"><a href="camerons_labblog/3236/UVVis_of_5mgmL_GFP.html">UV-Vis of 5mg/mL GFP</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2788/GFP_2_mgmL.html">GFP (~2 mg/mL)</a></td><td class="table_st">N/a</td><td class="table_st"><a href="camerons_labblog/3239/UVVis_of_2mgmL_GFP.html">UV-Vis of 2mg/mL GFP</a></td></tr></table><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3226/UVVis_of_5mgmL_Lysozyme.html">UV-Vis of 5mg/mL Lysozyme</a>;<a href="camerons_labblog/3229/UVVis_of_2mgmL_Lysozyme.html">UV-Vis of 2mg/mL Lysozyme</a>;<a href="camerons_labblog/3230/UVVis_of_20mgmL_GFP.html">UV-Vis of 20mg/mL GFP</a>;<a href="camerons_labblog/3233/UVVis_of_10mgmL_GFP.html">UV-Vis of 10mg/mL GFP</a>;<a href="camerons_labblog/3236/UVVis_of_5mgmL_GFP.html">UV-Vis of 5mg/mL GFP</a>;<a href="camerons_labblog/3239/UVVis_of_2mgmL_GFP.html">UV-Vis of 2mg/mL GFP</a>;<a href="camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.html">UV-Vis of 20mg/mL Lysozyme</a>;<a href="camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.html">UV-Vis of 10mg/mL Lysozyme</a>;
]]></html><datestamp>2009-02-09T14:45:03+00:00</datestamp><timestamp>2009-02-09T15:38:25+00:00</timestamp><blog>5</blog><key>de3c0448e4c1fe295991582a625a8acf</key><metadata><procedure>UV-Vis</procedure></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/1a6</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.png</format></formats><comments/></post><post><id>3220</id><rid>3222</rid><title>UV-Vis of 20mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]378[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=378','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=378&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:32:00+00:00</datestamp><timestamp>2009-02-09T15:32:26+00:00</timestamp><blog>5</blog><key>ebcdde308ba7f83cf0cd68536076dc00</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/378.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1a7</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3220/UVVis_of_20mgmL_Lysozyme.png</format></formats><comments/></post><post><id>3223</id><rid>3225</rid><title>UV-Vis of 10mg/mL Lysozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[From [blog]3219[/blog][data]380[/data]]]></content><html><![CDATA[<b>Data Type:</b> UV-Vis<br />
From <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=380','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=380&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;
]]></html><datestamp>2009-02-09T15:32:51+00:00</datestamp><timestamp>2009-02-09T15:33:09+00:00</timestamp><blog>5</blog><key>4a7b4800847304ae2d263ea4cf6797b9</key><metadata><data_type>UV-Vis</data_type></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/380.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/1a8</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/3223/UVVis_of_10mgmL_Lysozyme.png</format></formats><comments/></post><post><id>2765</id><rid>2765</rid><title>Preparation of DMPC vesicles</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[DMPC (20.6 mg) was dissolved in 10 mL of chloroform and transferred to a conical flask. The chloroform was evaporated under a stream of dry nitrogen. ]]></content><html><![CDATA[<b>Project:</b> Reaction_centre<br />
DMPC (20.6 mg) was dissolved in 10 mL of chloroform and transferred to a conical flask. The chloroform was evaporated under a stream of dry nitrogen. <br/>
]]></html><datestamp>2009-02-01T12:48:21+00:00</datestamp><timestamp>2009-02-01T12:48:21+00:00</timestamp><blog>5</blog><key>5ec7dc98f5b23c7438ebcbb3f844376e</key><metadata><project>Reaction_centre</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/14b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2765/Preparation_of_DMPC_vesicles.png</format></formats><comments/></post><post><id>2774</id><rid>2775</rid><title>Exchange of prBC into bOG</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Following the procedure from http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/5591 for detergent exchange.
Stock solution of 20 mM Tris-HCl pH 8 is available in the lab. Will use this as the base for buffers.
[blog]2767[/blog] (0.4104 g) was dissolved in 70 mL of Tris buffer.
Approx 1.5 mL of prBC sample was loaded onto a DEAE-FF column equilibrated in Tris buffer. The column was loaded and then washed with ~50 mL of buffer. The column was then exchanged into Tris buffer with 20 mM [blog]2767[/blog] and the protein attempted to be eluted with Tris buffer with [blog]2767[/blog] plus 200 mM NaCl.
Took four fractions and did UV absorbance but no significant peak at ~800 expected for prBC. Also no visible purple throughout run which suggests protein did not bind to column.]]></content><html><![CDATA[<b>Project:</b> Reaction_centre<br />
Following the procedure from <a href="http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/5591">http://chemtools.chem.soton.ac.uk/projects/blog/blogs.php/bit_id/5591</a> for detergent exchange.<br style="clear:left;"/><br style="clear:left;"/>Stock solution of 20 mM Tris-HCl pH 8 is available in the lab. Will use this as the base for buffers.<br style="clear:left;"/><br style="clear:left;"/><a href="lab_materials/2767/beta_octyl_glucoside.html">beta octyl glucoside</a> (0.4104 g) was dissolved in 70 mL of Tris buffer.<br style="clear:left;"/><br style="clear:left;"/>Approx 1.5 mL of prBC sample was loaded onto a DEAE-FF column equilibrated in Tris buffer. The column was loaded and then washed with ~50 mL of buffer. The column was then exchanged into Tris buffer with 20 mM <a href="lab_materials/2767/beta_octyl_glucoside.html">beta octyl glucoside</a> and the protein attempted to be eluted with Tris buffer with <a href="lab_materials/2767/beta_octyl_glucoside.html">beta octyl glucoside</a> plus 200 mM NaCl.<br style="clear:left;"/><br style="clear:left;"/>Took four fractions and did UV absorbance but no significant peak at ~800 expected for prBC. Also no visible purple throughout run which suggests protein did not bind to column.<br/>
]]></html><datestamp>2009-02-01T15:00:06+00:00</datestamp><timestamp>2009-02-01T16:00:51+00:00</timestamp><blog>5</blog><key>7900c0c44ee9d7bb38908a62e9286d3c</key><metadata><project>Reaction_centre</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/14e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2774/Exchange_of_prBC_into_bOG.png</format></formats><comments/></post><post><id>2778</id><rid>2780</rid><title>Lysozyme (~20 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Solution of lysozyme at approx 20 mg/mL from [blog]2779[/blog]]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Solution of lysozyme at approx 20 mg/mL from <a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;<a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a>;
]]></html><datestamp>2009-02-01T16:30:33+00:00</datestamp><timestamp>2009-02-01T16:31:00+00:00</timestamp><blog>5</blog><key>d74dae5f57c53383af1c46830b4a7c4a</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/151</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2778/Lysozyme_20_mgmL.png</format></formats><comments/></post><post><id>2782</id><rid>2785</rid><title>GFP (~20 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 20 mg/mL GFP prepared in [blog]2783[/blog]]]></content><html><![CDATA[<b>Project:</b> SAS_standard<br />
Approx 20 mg/mL GFP prepared in <a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a>;
]]></html><datestamp>2009-02-01T16:32:27+00:00</datestamp><timestamp>2009-02-01T16:57:28+00:00</timestamp><blog>5</blog><key>f5bf61d81d0fc1b347129080e130947a</key><metadata><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/153</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2782/GFP_20_mgmL.png</format></formats><comments/></post><post><id>2786</id><rid>2786</rid><title>GFP (~10mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 10 mg/mL GFP prepared in [blog]2783[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 10 mg/mL GFP prepared in <a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a>;
]]></html><datestamp>2009-02-01T16:57:42+00:00</datestamp><timestamp>2009-02-01T16:57:42+00:00</timestamp><blog>5</blog><key>a63ccd63accd70b1f7208c9834237f86</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/155</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2786/GFP_10mgmL.png</format></formats><comments/></post><post><id>2787</id><rid>2787</rid><title>GFP (~5 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 5 mg/mL GFP prepared in [blog]2783[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 5 mg/mL GFP prepared in <a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a>;
]]></html><datestamp>2009-02-01T16:58:14+00:00</datestamp><timestamp>2009-02-01T16:58:14+00:00</timestamp><blog>5</blog><key>4840dc712155fa25b8322d7238dacad1</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/156</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2787/GFP_5_mgmL.png</format></formats><comments/></post><post><id>2788</id><rid>2788</rid><title>GFP (~2 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 2 mg/mL GFP prepared in [blog]2783[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 2 mg/mL GFP prepared in <a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2783/Preparation_of_GFP_solutions_for_I22_SAXS.html">Preparation of GFP solutions for I22 SAXS</a>;
]]></html><datestamp>2009-02-01T16:58:42+00:00</datestamp><timestamp>2009-02-01T16:58:42+00:00</timestamp><blog>5</blog><key>78b052f22d66cf8616c4f6bb66be34ea</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/157</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2788/GFP_2_mgmL.png</format></formats><comments/></post><post><id>2790</id><rid>2790</rid><title>Lysozyme (~10 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 10 mg/mL lysozyme prepared in [blog]2779[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 10 mg/mL lysozyme prepared in <a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;<a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a>;
]]></html><datestamp>2009-02-01T17:01:00+00:00</datestamp><timestamp>2009-02-01T17:01:00+00:00</timestamp><blog>5</blog><key>5a6577b6f4e1354c55972f5b346d96f6</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/158</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2790/Lysozyme_10_mgmL.png</format></formats><comments/></post><post><id>2791</id><rid>2791</rid><title>Lysozyme (~5 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 5 mg/mL lysozyme prepared in [blog]2779[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 5 mg/mL lysozyme prepared in <a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;<a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a>;
]]></html><datestamp>2009-02-01T17:01:26+00:00</datestamp><timestamp>2009-02-01T17:01:26+00:00</timestamp><blog>5</blog><key>b95c8b6aec4c44624d72a299a4095ac1</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/159</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2791/Lysozyme_5_mgmL.png</format></formats><comments/></post><post><id>2792</id><rid>2792</rid><title>Lysozyme (~2 mg/mL)</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Approx 2 mg/mL lysozyme prepared in [blog]2779[/blog]
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
Approx 2 mg/mL lysozyme prepared in <a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="/camerons_labblog/3219/UVVis_of_Lysozyme_and_GFP_samples_from_I22_SAXS.html">UV-Vis of Lysozyme and GFP samples from I22 SAXS</a>;<a href="/camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;<a href="/camerons_labblog/2779/Preparation_of_lysozyme_solutions.html">Preparation of lysozyme solutions</a>;
]]></html><datestamp>2009-02-01T17:01:52+00:00</datestamp><timestamp>2009-02-01T17:01:52+00:00</timestamp><blog>5</blog><key>3390efdf4bdc11c29c4bd74985a75f27</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/15a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2792/Lysozyme_2_mgmL.png</format></formats><comments/></post><post><id>2816</id><rid>2819</rid><title>Tris-HCl pH 8.0 buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[tris-hcl pH 8.0 buffer]]></content><html><![CDATA[<b>Project:</b> SAS_standard<br />
<b>Material:</b> Solution<br />
tris-hcl pH 8.0 buffer<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T14:16:52+00:00</datestamp><timestamp>2009-02-02T14:20:16+00:00</timestamp><blog>5</blog><key>e49ed9eeb95366c0c5f79c0e8ea1d898</key><metadata><project>SAS_standard</project><material>Solution</material></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/15b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2816/TrisHCl_pH_80_buffer.png</format></formats><comments/></post><post><id>2822</id><rid>2822</rid><title>SAXS on Tris-HCl pH 8.0 buffer</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A02001.202[col]A02000.DAT[/row]
[/table]
]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A02001.202</td><td class="table_st">A02000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T14:30:45+00:00</datestamp><timestamp>2009-02-02T14:30:45+00:00</timestamp><blog>5</blog><key>43ebe9b1ac30baa256f06d098583cccb</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/15e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.png</format></formats><comments/></post><post><id>2838</id><rid>2842</rid><title>Lysozyme 10 mg/mL with attenutor</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A10000.202[col]A10000.DAT[/row]
[/table]
[data]314[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A10000.202</td><td class="table_st">A10000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=314','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=314&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T17:22:34+00:00</datestamp><timestamp>2009-02-02T17:26:28+00:00</timestamp><blog>5</blog><key>394326e79f09d27e1f2f8646937f8569</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/314.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/167</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2838/Lysozyme_10_mgmL_with_attenutor.png</format></formats><comments/></post><post><id>2834</id><rid>2846</rid><title>Lysozyme 5mg/mL with attenuator</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A09000.202[col]A09000.DAT[/row]
[/table]
[data]318[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A09000.202</td><td class="table_st">A09000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=318','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=318&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T16:50:50+00:00</datestamp><timestamp>2009-02-02T17:27:18+00:00</timestamp><blog>5</blog><key>c71628d0624d2f869fae8e60705f54e7</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/318.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/164</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.png</format></formats><comments/></post><post><id>2835</id><rid>2847</rid><title>Lysozyme 2mg/mL with attenuator</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A08000.202[col]A08000.DAT[/row]
[/table][data]316[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A08000.202</td><td class="table_st">A08000.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=316','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=316&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T16:51:59+00:00</datestamp><timestamp>2009-02-02T17:27:32+00:00</timestamp><blog>5</blog><key>2202e93ef954831358a71d408d1bf4ae</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/316.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/165</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.png</format></formats><comments/></post><post><id>2833</id><rid>2849</rid><title>Buffer with attentuator</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A07000.202[col]A07000.DAT[/row]
[/table]
[data]320[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A07000.202</td><td class="table_st">A07000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=320','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=320&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T16:50:11+00:00</datestamp><timestamp>2009-02-02T17:28:05+00:00</timestamp><blog>5</blog><key>a4018a418fca531afe19bb3085eb53a7</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/320.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/163</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2833/Buffer_with_attentuator.png</format></formats><comments/></post><post><id>2832</id><rid>2851</rid><title>Lysozyme 20 mg/mL w/o attenutor</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A05000.202[col]A05000.DAT[/row]
[/table]
[data]322[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A05000.202</td><td class="table_st">A05000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=322','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=322&width=100&height=75&thumb=1" class="dataPic"></a></div><br/>
]]></html><datestamp>2009-02-02T16:49:20+00:00</datestamp><timestamp>2009-02-02T17:32:45+00:00</timestamp><blog>5</blog><key>19ada060b585476abfd99ee49d9f3321</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/322.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/162</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2832/Lysozyme_20_mgmL_wo_attenutor.png</format></formats><comments/></post><post><id>2831</id><rid>2853</rid><title>Lysozyme 5mg/mL w/o attenuator</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A04000.202[col]A04000.DAT[/row]
[/table]
[data]324[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A04000.202</td><td class="table_st">A04000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=324','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=324&width=100&height=75&thumb=1" class="dataPic"></a></div><br/>
]]></html><datestamp>2009-02-02T16:47:48+00:00</datestamp><timestamp>2009-02-02T17:33:14+00:00</timestamp><blog>5</blog><key>1e8e7aa545f63a1d7eb796ab1593b724</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/324.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/161</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2831/Lysozyme_5mgmL_wo_attenuator.png</format></formats><comments/></post><post><id>2824</id><rid>2855</rid><title>First run on 2 mg/ml Lyzozyme</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A03000.202[col]A03000.DAT[/row]
[/table]
[data]326[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A03000.202</td><td class="table_st">A03000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=326','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=326&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2825/SAXS_on_I22_template.html">SAXS on I22 template</a>;
]]></html><datestamp>2009-02-02T15:56:56+00:00</datestamp><timestamp>2009-02-02T17:33:40+00:00</timestamp><blog>5</blog><key>553fb24951904a864a62cb4b3ee99bcb</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/326.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/15f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2824/First_run_on_2_mgml_Lyzozyme.png</format></formats><comments/></post><post><id>2837</id><rid>2857</rid><title>Lysozyme 20 mg/mL with attenutor</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A11000.202[col]A11000.DAT[/row]
[/table]
[data]328[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A11000.202</td><td class="table_st">A11000.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=328','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=328&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T17:22:01+00:00</datestamp><timestamp>2009-02-02T17:41:04+00:00</timestamp><blog>5</blog><key>39844854a67d01844e5328ac8b3efd18</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/328.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/166</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2837/Lysozyme_20_mgmL_with_attenutor.png</format></formats><comments/></post><post><id>2858</id><rid>2858</rid><title>GFP 2mg/mL</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A12000.202[col]A12001.DAT[/row]
[/table]
]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A12000.202</td><td class="table_st">A12001.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T17:44:08+00:00</datestamp><timestamp>2009-02-02T17:44:08+00:00</timestamp><blog>5</blog><key>fab53c0d6dd43f8255c9c1b14c294472</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/168</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2858/GFP_2mgmL.png</format></formats><comments/></post><post><id>2859</id><rid>2862</rid><title>second 10 frames of 2 mg/mL GFP</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A13000.202[col]A13001.DAT[/row]
[/table]
[data]330[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A13000.202</td><td class="table_st">A13001.DAT</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=330','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=330&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T18:30:15+00:00</datestamp><timestamp>2009-02-02T18:31:31+00:00</timestamp><blog>5</blog><key>6421cb054135fcae985648d16ee9801a</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/330.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/169</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2859/second_10_frames_of_2_mgmL_GFP.png</format></formats><comments/></post><post><id>2860</id><rid>2867</rid><title>GFP 5 mg/mL</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A14000.202[col]A14001.202[/row]
[/table]
[data]332[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A14000.202</td><td class="table_st">A14001.202</td></tr></table><br style="clear:left;"/><br style="clear:left;"/><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=332','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=332&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T18:30:48+00:00</datestamp><timestamp>2009-02-02T18:54:11+00:00</timestamp><blog>5</blog><key>15817596e54a184d8c8d5addc34a2977</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/332.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/16a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2860/GFP_5_mgmL.png</format></formats><comments/></post><post><id>2872</id><rid>2873</rid><title>0.25 mg/mL GluR0 with glutamate</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[0.25 mg/mL GluR0 with glutamate prepared by Michael]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
0.25 mg/mL GluR0 with glutamate prepared by Michael<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2886/SAXS_runs_of_GluR0.html">SAXS runs of GluR0</a>;
]]></html><datestamp>2009-02-02T19:28:39+00:00</datestamp><timestamp>2009-02-02T19:28:54+00:00</timestamp><blog>5</blog><key>2447829cf1671de53d9ad843ddec4958</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/16e</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.png</format></formats><comments/></post><post><id>2874</id><rid>2875</rid><title>0.5 mg/mL GluR0 with glutamate</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[0.5 mg/mL GluR0 with glutamate prepared by Michael]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> SAS_standard<br />
0.5 mg/mL GluR0 with glutamate prepared by Michael<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2886/SAXS_runs_of_GluR0.html">SAXS runs of GluR0</a>;
]]></html><datestamp>2009-02-02T19:29:27+00:00</datestamp><timestamp>2009-02-02T19:29:40+00:00</timestamp><blog>5</blog><key>30185546126fce4f82fc60cbcdb63169</key><metadata><material>Solution</material><project>SAS_standard</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/16f</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.png</format></formats><comments/></post><post><id>2863</id><rid>2878</rid><title>GFP 10mg/mL</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A15000.202[col]A15001.DAT[/row]
[/table][data]334[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A15000.202</td><td class="table_st">A15001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=334','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=334&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T18:37:12+00:00</datestamp><timestamp>2009-02-02T19:36:27+00:00</timestamp><blog>5</blog><key>1fc6a8cbc2fa410ef85f848c319d6cc6</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/334.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/16b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2863/GFP_10mgmL.png</format></formats><comments/></post><post><id>2864</id><rid>2880</rid><title>GFP 20mg/mL</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A16000.202[col]A16001.DAT[/row]
[/table][data]336[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> SAS_standard<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A16000.202</td><td class="table_st">A16001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=336','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=336&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2868/Further_lysozyme_runs.html">Further lysozyme runs</a>;
]]></html><datestamp>2009-02-02T18:37:38+00:00</datestamp><timestamp>2009-02-02T19:36:54+00:00</timestamp><blog>5</blog><key>b4f6fdd92687ab2aea0e3a97ca61c9d0</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>SAS_standard</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/336.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/16c</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2864/GFP_20mgmL.png</format></formats><comments/></post><post><id>2825</id><rid>2890</rid><title>SAXS on I22 template</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.
[table]
[row]Sample[col]Frames[col]Exposure time [col]I22 Raw Run #[col]Data[/row]
[row][blog]2816[/blog][col]1[col]10[col]A2001.202[col][blog]2822[/blog][/row]
[row][blog]2792[/blog][col]10[col]10[col]A03000.202[col][blog]2824[/blog][/row]
[row][blog]2791[/blog][col]10[col]10[col]A04000.202[col][blog][/blog][/row]
[row][blog]2778[/blog][col]10[col]10[col]A05000.202[col][blog][/blog][/row]
[row][blog]2816[/blog][col]10[col]10[col]A06000.202[col][blog][/blog][/row]
[row][blog]2816[/blog][col]30[col]30[col]A07000.202[col][blog]2833[/blog][/row]
[row][blog]2792[/blog][col]30[col]30[col]A08000.202[col][blog]2835[/blog][/row]
[row][blog]2791[/blog][col]30[col]30[col]A09000.202[col][blog]2834[/blog][/row]
[/table]
After first four runs put in attentuator (10x, 0.5 mm of Al)]]></content><html><![CDATA[<b>Procedure:</b> SAXS<br />
<b>Instrument:</b> I22<br />
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Frames</td><td class="table_st">Exposure time </td><td class="table_st">I22 Raw Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/2816/TrisHCl_pH_80_buffer.html">Tris-HCl pH 8.0 buffer</a></td><td class="table_st">1</td><td class="table_st">10</td><td class="table_st">A2001.202</td><td class="table_st"><a href="camerons_labblog/2822/SAXS_on_TrisHCl_pH_80_buffer.html">SAXS on Tris-HCl pH 8.0 buffer</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2792/Lysozyme_2_mgmL.html">Lysozyme (~2 mg/mL)</a></td><td class="table_st">10</td><td class="table_st">10</td><td class="table_st">A03000.202</td><td class="table_st"><a href="camerons_labblog/2824/First_run_on_20_mgml_Lyzozyme.html">First run on 20 mg/ml Lyzozyme</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2791/Lysozyme_5_mgmL.html">Lysozyme (~5 mg/mL)</a></td><td class="table_st">10</td><td class="table_st">10</td><td class="table_st">A04000.202</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/2778/Lysozyme_20_mgmL.html">Lysozyme (~20 mg/mL)</a></td><td class="table_st">10</td><td class="table_st">10</td><td class="table_st">A05000.202</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/2816/TrisHCl_pH_80_buffer.html">Tris-HCl pH 8.0 buffer</a></td><td class="table_st">10</td><td class="table_st">10</td><td class="table_st">A06000.202</td><td class="table_st"></td></tr><tr><td class="table_st"><a href="camerons_labblog/2816/TrisHCl_pH_80_buffer.html">Tris-HCl pH 8.0 buffer</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A07000.202</td><td class="table_st"><a href="camerons_labblog/2833/Buffer_with_attentuator.html">Buffer with attentuator</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2792/Lysozyme_2_mgmL.html">Lysozyme (~2 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A08000.202</td><td class="table_st"><a href="camerons_labblog/2835/Lysozyme_2mgmL_with_attenuator.html">Lysozyme 2mg/mL with attenuator</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2791/Lysozyme_5_mgmL.html">Lysozyme (~5 mg/mL)</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A09000.202</td><td class="table_st"><a href="camerons_labblog/2834/Lysozyme_5mgmL_with_attenuator.html">Lysozyme 5mg/mL with attenuator</a></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>After first four runs put in attentuator (10x, 0.5 mm of Al)<br/>
]]></html><datestamp>2009-02-02T16:22:56+00:00</datestamp><timestamp>2009-02-02T22:46:49+00:00</timestamp><blog>5</blog><key>a5964748f42db06e1f8ba9e3084b6c58</key><metadata><procedure>SAXS</procedure><instrument>I22</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/160</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2825/SAXS_on_I22_template.png</format></formats><comments/></post><post><id>2896</id><rid>2897</rid><title>GluR0 buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[GluR0 buffer prepared by Michael]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> GluR0<br />
GluR0 buffer prepared by Michael<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-02T23:00:41+00:00</datestamp><timestamp>2009-02-02T23:00:53+00:00</timestamp><blog>5</blog><key>992e6867cafaa0d8649329bcea09c290</key><metadata><material>Solution</material><project>GluR0</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/173</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2896/GluR0_buffer.png</format></formats><comments/></post><post><id>2886</id><rid>2899</rid><title>SAXS runs of GluR0</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.
[table]
[row]Sample[col]Frames[col]Exposure time [col]I22 Raw Run #[col]Data[/row]
[row][blog]2872[/blog][col]30[col]30[col]A17000.202[col][blog]2876[/blog][/row]
[row][blog]2874[/blog][col]30[col]30[col]A1800.202[col][blog]2881[/blog][/row]
[/table]
SAXS from 0.5 mg/mL seems poor compared to 0.25 mg/mL. Are these the wrong way around?]]></content><html><![CDATA[<b>Procedure:</b> SAXS<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
The following samples were run in 1mm SAXS cells on I22. Detector was at 5m and q range standardised against silver behenate.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Frames</td><td class="table_st">Exposure time </td><td class="table_st">I22 Raw Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/2872/025_mgmL_GluR0_with_glutamate.html">0.25 mg/mL GluR0 with glutamate</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A17000.202</td><td class="table_st"><a href="camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.html">GluR0 SAXS 0.25 mg/mL + glu first run</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2874/05_mgmL_GluR0_with_glutamate.html">0.5 mg/mL GluR0 with glutamate</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A1800.202</td><td class="table_st"><a href="camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.html">SAXS of 0.5 mg/mL GluR0 with glutamate</a></td></tr></table><br style="clear:left;"/><br style="clear:left;"/>SAXS from 0.5 mg/mL seems poor compared to 0.25 mg/mL. Are these the wrong way around?<br/>
]]></html><datestamp>2009-02-02T22:45:48+00:00</datestamp><timestamp>2009-02-02T23:02:27+00:00</timestamp><blog>5</blog><key>eb821b34fa198904c8eba01e48ad41a9</key><metadata><procedure>SAXS</procedure><instrument>I22</instrument><project>GluR0</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/172</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2886/SAXS_runs_of_GluR0.png</format></formats><comments/></post><post><id>2900</id><rid>2901</rid><title>GluR0 1mg/mL no glutamate</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[GluR0 1mg/mL no glutamate prepared by Michael]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> GluR0<br />
GluR0 1mg/mL no glutamate prepared by Michael<br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-02T23:11:51+00:00</datestamp><timestamp>2009-02-02T23:12:00+00:00</timestamp><blog>5</blog><key>734b6b262e779565e1f7fe69840bfdc1</key><metadata><material>Solution</material><project>GluR0</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/174</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2900/GluR0_1mgmL_no_glutamate.png</format></formats><comments/></post><post><id>2903</id><rid>2903</rid><title>0.5 mg/mL GluR0 no glutamate</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[0.5 mg/mL GluR0 no glutamate prepared by Michael
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> GluR0<br />
0.5 mg/mL GluR0 no glutamate prepared by Michael<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-02T23:12:36+00:00</datestamp><timestamp>2009-02-02T23:12:36+00:00</timestamp><blog>5</blog><key>4568c895e57bb3ba9a7dd42c8b70b69f</key><metadata><material>Solution</material><project>GluR0</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/175</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.png</format></formats><comments/></post><post><id>2904</id><rid>2904</rid><title>0.25 mg/mL GluR0 no glutamate</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[0.25 mg/mL GluR0 no glutamate prepared by Michael
]]></content><html><![CDATA[<b>Material:</b> Solution<br />
<b>Project:</b> GluR0<br />
0.25 mg/mL GluR0 no glutamate prepared by Michael<br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br style="clear:left;"/><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-02T23:12:54+00:00</datestamp><timestamp>2009-02-02T23:12:54+00:00</timestamp><blog>5</blog><key>b6ce106577103b1f20f598c56b090f8f</key><metadata><material>Solution</material><project>GluR0</project></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/176</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.png</format></formats><comments/></post><post><id>2881</id><rid>2906</rid><title>SAXS of 0.5 mg/mL GluR0 with glutamate</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A18000.202[col]A18001.DAT[/row]
[/table][data]338[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A18000.202</td><td class="table_st">A18001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=338','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=338&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2886/SAXS_runs_of_GluR0.html">SAXS runs of GluR0</a>;
]]></html><datestamp>2009-02-02T19:51:10+00:00</datestamp><timestamp>2009-02-02T23:14:13+00:00</timestamp><blog>5</blog><key>adcfa3381f32ae7bb8b5958ab1a47236</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/338.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/171</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2881/SAXS_of_05_mgmL_GluR0_with_glutamate.png</format></formats><comments/></post><post><id>2876</id><rid>2908</rid><title>GluR0 SAXS 0.25 mg/mL + glu first run</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A17000.202[col]A17001.DAT[/row]
[/table][data]340[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A17000.202</td><td class="table_st">A17001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=340','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=340&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2886/SAXS_runs_of_GluR0.html">SAXS runs of GluR0</a>;
]]></html><datestamp>2009-02-02T19:34:56+00:00</datestamp><timestamp>2009-02-02T23:14:47+00:00</timestamp><blog>5</blog><key>8f07f1b876b459e529b5a7ab67a4366a</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/340.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/170</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2876/GluR0_SAXS_025_mgmL__glu_first_run.png</format></formats><comments/></post><post><id>2909</id><rid>2911</rid><title>SAXS of GLuR0 buffer</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A19000.202[col]A19001.DAT[/row]
[/table][data]342[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A19000.202</td><td class="table_st">A19001.DAT</td></tr></table><div style="float:left;"><div class="dataPic datathumb" onclick="javascript:var blob = window.open('/data/342.html','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><div style="background-repeat: no-repeat; background-position: center center; background-image: url(/getdata.php?bit=342&width=100&height=75&thumb=1)"></div>A19001.DAT</div></div><br/><b>This Post is Linked By:</b> <a href="http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.html">Reprocessing SAXS data from Feb 09</a>;
]]></html><datestamp>2009-02-02T23:29:56+00:00</datestamp><timestamp>2009-02-02T23:30:16+00:00</timestamp><blog>5</blog><key>a3c7703c2a2e3cc3e6a1bc2047443521</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/342.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/177</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2909/SAXS_of_GLuR0_buffer.png</format></formats><comments/></post><post><id>2912</id><rid>2914</rid><title>SAXS of 1 mg/mL GluR0 no glutamate</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A20000.202[col]A20001.DAT[/row]
[/table][data]344[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A20000.202</td><td class="table_st">A20001.DAT</td></tr></table><div style="float:left;"><div class="dataPic datathumb" onclick="javascript:var blob = window.open('/data/344.html','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><div style="background-repeat: no-repeat; background-position: center center; background-image: url(/getdata.php?bit=344&width=100&height=75&thumb=1)"></div>A20001.DAT</div></div><br/><b>This Post is Linked By:</b> <a href="http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;<a href="http://biolab.isis.rl.ac.uk/camerons_labblog/11586/Reprocessing_SAXS_data_from_Feb_09.html">Reprocessing SAXS data from Feb 09</a>;
]]></html><datestamp>2009-02-02T23:31:15+00:00</datestamp><timestamp>2009-02-02T23:39:03+00:00</timestamp><blog>5</blog><key>20b44c25f7935af50c6571a7b5f40445</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/344.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/178</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.png</format></formats><comments/></post><post><id>2915</id><rid>2919</rid><title>SAXS of GluR0 0.5 mg/mL no glutamate</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A21000.202[col]A21001.DAT[/row]
[/table][data]346[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A21000.202</td><td class="table_st">A21001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=346','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=346&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-02T23:48:42+00:00</datestamp><timestamp>2009-02-02T23:58:30+00:00</timestamp><blog>5</blog><key>f2e56a1bd2b5f44bb846dc75170dc51e</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/346.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/179</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.png</format></formats><comments/></post><post><id>2921</id><rid>2922</rid><title>SAXS on GluR) in capillaries</title><section>Procedures</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.
[table]
[row]Sample[col]Frames[col]Exposure time [col]I22 Raw Run #[col]Data[/row]
[row][blog]2896[/blog][col]30[col]30[col]A19000.202[col][blog]2909[/blog][/row]
[row][blog]2900[/blog][col]30[col]30[col]A20000.202[col][blog]2912[/blog][/row]
[row][blog]2903[/blog][col]30[col]30[col]A21000.202[col][blog]2915[/blog][/row]
[row][blog]2904[/blog][col]30[col]30[col]22000.202[col][blog]2920[/blog][/row]
[/table]]]></content><html><![CDATA[<b>Procedure:</b> SAXS<br />
<b>Instrument:</b> I22<br />
The following samples were run in 1 mm capillary cells on I22. Detector was at 5m and q range standardised against silver behenate.<br style="clear:left;"/><br style="clear:left;"/><table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Sample</td><td class="table_st">Frames</td><td class="table_st">Exposure time </td><td class="table_st">I22 Raw Run #</td><td class="table_st">Data</td></tr><tr><td class="table_st"><a href="camerons_labblog/2896/GluR0_buffer.html">GluR0 buffer</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A19000.202</td><td class="table_st"><a href="camerons_labblog/2909/SAXS_of_GLuR0_buffer.html">SAXS of GLuR0 buffer</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2900/GluR0_1mgmL_no_glutamate.html">GluR0 1mg/mL no glutamate</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A20000.202</td><td class="table_st"><a href="camerons_labblog/2912/SAXS_of_1_mgmL_GluR0_no_glutamate.html">SAXS of 1 mg/mL GluR0 no glutamate</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2903/05_mgmL_GluR0_no_glutamate.html">0.5 mg/mL GluR0 no glutamate</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">A21000.202</td><td class="table_st"><a href="camerons_labblog/2915/SAXS_of_GluR0_05_mgmL_no_glutamate.html">SAXS of GluR0 0.5 mg/mL no glutamate</a></td></tr><tr><td class="table_st"><a href="camerons_labblog/2904/025_mgmL_GluR0_no_glutamate.html">0.25 mg/mL GluR0 no glutamate</a></td><td class="table_st">30</td><td class="table_st">30</td><td class="table_st">22000.202</td><td class="table_st"><a href="camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.html">SAXS of GluR0 0.25 mg/mL no glutamate</a></td></tr></table><br/>
]]></html><datestamp>2009-02-03T00:12:57+00:00</datestamp><timestamp>2009-02-03T00:13:10+00:00</timestamp><blog>5</blog><key>5b7522d772e34d4d8d3dea009e843a5b</key><metadata><procedure>SAXS</procedure><instrument>I22</instrument></metadata><links><uri>http://biolab.isis.rl.ac.uk/uri/17b</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2921/SAXS_on_GluR_in_capillaries.png</format></formats><comments/></post><post><id>2920</id><rid>2924</rid><title>SAXS of GluR0 0.25 mg/mL no glutamate</title><section>Data</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[[table]
[row]Raw data file[col]Data file[/row]
[row]A22000.202[col]A22001.DAT[/row]
[/table][data]348[/data]]]></content><html><![CDATA[<b>Data Type:</b> SAXS_Diamond<br />
<b>Instrument:</b> I22<br />
<b>Project:</b> GluR0<br />
<table class="table_st" cellspacing="0"><br style="clear:left;"/><tr><td class="table_st">Raw data file</td><td class="table_st">Data file</td></tr><tr><td class="table_st">A22000.202</td><td class="table_st">A22001.DAT</td></tr></table><div style="float:left;"><a href="javascript:var blob = window.open('/data_pre.php?id=348','popup','scrollbars=1;menubar=no,height=750,width=680,resizable=yes,toolbar=no,location=no,status=no');"><img src="/getdata.php?bit=348&width=100&height=75&thumb=1" class="dataPic"></a></div><br/><b>This Post is Linked By:</b> <a href="camerons_labblog/2921/SAXS_on_GluR_in_capillaries.html">SAXS on GluR) in capillaries</a>;
]]></html><datestamp>2009-02-03T00:04:04+00:00</datestamp><timestamp>2009-02-03T00:20:36+00:00</timestamp><blog>5</blog><key>a2eff19bd4647c55f2481b767d965a42</key><metadata><data_type>SAXS_Diamond</data_type><instrument>I22</instrument><project>GluR0</project></metadata><attached_data><data>http://biolab.isis.rl.ac.uk/data/348.xml</data></attached_data><links><uri>http://biolab.isis.rl.ac.uk/uri/17a</uri><permalink>http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.html</permalink></links><formats><format type="text/html">http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.html</format><format type="text/xml">http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.xml</format><format type="image/png">http://biolab.isis.rl.ac.uk/camerons_labblog/2920/SAXS_of_GluR0_025_mgmL_no_glutamate.png</format></formats><comments/></post><post><id>2926</id><rid>2926</rid><title>Tus buffer</title><section>Materials</section><author><username>cameron.neylon.myopenid.com</username><name>Cameron Neylon</name></author><content><![CDATA[Tus buffer prepared by Shubbie