scBRIE2

Repository for scRNA-Seq splice variant detection mapping using STARsolo and BRIE2

• The scripts to run the pipeline are inside scripts.

Experimental Design

The data have been produced using the snRNA-seq2 protocol in the Martinez Lab at Helmholtz Pioneer Campus as previously described in https://www.nature.com/articles/s41467-021-24543-5. Briefly, single nuclei were isolated from young (3 months) and old (22 months) mice livers, divided based on ploidy and sorted into 384-well plates. The plates were processed with a modified protocol and sequenced to saturation. Additional details can be found in the metadata file.

Mapping using STARsolo

The index was build from Gencode M17 version of the mouse genome for compatibility with BRIE2 annotations.