Molecular Biology: Plasmid Preparation buffers

[steven-albanese]

There are a number of buffers that we'll need to make for the plasmid prep protocol

Buffer Name	Components
Resuspension Buffer	50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 100 µg/mL RNase A
Lysis Buffer	200 mM NaOH, 1% SDS
Neutralization Buffer	3.0 M Potassium Acetate, pH 5.5
Binding Buffer	6 M Guanidine-HCl
Washer Buffer	80% Ethanol
Elution Buffer	10 mM Tris-HCl, pH 8.0, 1 mM EDTA or DNase, RNase free water

We will likely not need huge amount of these buffers, so I'm not sure that the Revo is the best option for right now! Do we have an estimate of time frame for them to implement the small volumes @Lucelenie? Otherwise, these looks like we might be able to make them by hand.