Otsu_ For_ Cell.m is used to segment sliced images into cells, while removeLargeArea.m is an additional defined function to ensure the normal operation of the segmentation code. Demo code is used to analyze and cluster the color information of cells，which includes RGBTernaryPlotDisplay、RGBTernaryPlotClustering_v1、RGB3dPlotDisplay、HSclustering. ningjudu.m is used to calculate cell cohesion and separation. DATA.xlsx is used to test the code results of color analysis.