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_HomoloGene_To_UniProt_ID_Retriever.py
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_HomoloGene_To_UniProt_ID_Retriever.py
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import shutil
import csv
import smtplib
import time
import requests
import pandas as pd
import xml.etree.ElementTree as ET
import os
# - 1) Need to write a function that opens Full [Insert Proteome] Human CSV - #
# - 2) Runs the HomoloGene Checker to get "Core" proteins - #
# - 3) Back checks with UniProt to get the "Core Protein IDs" - #
# - 4) Stores "UniProt ID, Gene Name, and Organism - #
### Declaring Global Variables here ###
studying = "Nucleolus"
db = "HomoloGene"
NCBIGeneList = []
# 1: "Human", 2: "Cow", 3: "Mouse", 4: "Zebrafish", 5: "Fly", 6: "Worm", 7: "Yeast", 8: "Frog"
organismTaxIDDict = {1: 9606, 2: 9913, 3: 10090, 4: 7955,
5: 7227, 6: 6239, 7: 4932, 8: 8364}
# Need to revise Yeast Dictionary for UniProt query
organismTaxIDDict_Revised = {1: 9606, 2: 9913, 3: 10090, 4: 7955,
5: 7227, 6: 6239, 7: 559292, 8: 8364}
organismNameDict = {1: "Human", 2: "Cow", 3: "Mouse", 4: "Zebrafish", 5: "Fly", 6: "Worm", 7: "Yeast", 8: "Frog"}
organismTaxIDNameDict = {9606: "Human", 9913: "Cow", 10090: "Mouse", 7955: "Zebrafish", 7227: "Fly", 6239: "Worm", 559292: "Yeast", 8364: "Frog"}
# Start running time
start_time = time.time()
## Input/Output Files Here ###
#PATHS
outputPathExcel = "_GUI/Core/"
if not os.path.exists('{path}{nameDir}/'.format(path=outputPathExcel, nameDir="Temp")):
os.makedirs('{path}{nameDir}/'.format(path=outputPathExcel, nameDir="Temp"))
tempPath = "{path}{Temp}/".format(path=outputPathExcel, Temp="Temp")
#CSV#
inputFile = "_GUI/UniProt_{studying}_Human.csv".format(studying=studying)
outputHomoloGene = "{output}_{study}_Core_HomoloGene_Checked.csv".format(study=studying,output=outputPathExcel)
resultFile = "{output}_{study}_Core_UnitProtID_Compiled.csv".format(output=outputPathExcel, study=studying)
failedFile = "{output}_{study}_Core_UniProtID_Failed.csv".format(output=outputPathExcel, study=studying)
## Functions ##
def RemoveExistingFiles(file):
for files in file:
if os.path.exists(files):
os.remove(files)
def WriteToCsv(path, row, fileMode):
with open(path, fileMode) as fd:
wr = csv.writer(fd, quoting=csv.QUOTE_ALL)
wr.writerow(row)
def SplitListEntry(item):
print(item)
splitItem = item.split('_')
print(splitItem)
splitItemByPeriod = splitItem[2].split('.')
print(splitItemByPeriod)
#Declared Variables
protName = splitItem[1]
idNum = splitItemByPeriod[-2]
return protName, idNum
def OpenCSV(inputFile):
columnList = ['Entry', 'Gene', 'Organism']
df = pd.read_csv(inputFile, usecols=columnList)
# Reformatting Entries if not already done
df = df[df['Entry'].notna()]
df = df[df['Gene'].notna()]
df['Gene'] = df['Gene'].str.upper()
df = df[df['Organism'].notna()]
df.drop_duplicates(subset="Gene",
keep="first", inplace=True)
gene = df['Gene'].tolist()
gene.pop(0)
return gene
def HomoloGeneCheck(lst):
for query in lst:
print("Checking HomoloGene for... ", query)
urlESearch = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esearch.fcgi?db={db}&term={" \
"query}&api_key=1eaf48f05dc4f266c714b46a2bd351e8fa09".format(db=db, query=query)
loopNum = 0
while loopNum < 3:
try:
# print(urlESearch)
r = requests.get(url=urlESearch, stream=False)
root = ET.fromstring(r.content)
# print(r.content) #prints the xml content
break
except:
loopNum +=1
idList = []
for child in root.iter('Id'):
idList.append(child.text)
# print("idList: ", idList)
for uid in idList:
urlESummary = "https://eutils.ncbi.nlm.nih.gov/entrez/eutils/esummary.fcgi?db={db}&id={" \
"uid}&version=2.0&api_key=1eaf48f05dc4f266c714b46a2bd351e8fa09".format(
db=db, uid=uid)
# print(urlESummary)
r = requests.get(url=urlESummary, stream=False)
root = ET.fromstring(r.content)
taxIdList = []
symblList = []
resultingSymblList = []
for taxName in root.iter('TaxId'):
taxIdList.append(int(taxName.text))
for symbl in root.iter('Symbol'):
symblList.append(symbl.text)
for i, taxID in enumerate(taxIdList):
if taxID in organismTaxIDDict.values() and taxID not in taxIdList[:i]:
resultingSymblList.append(symblList[i])
if len(resultingSymblList) == len(organismTaxIDDict):
print(query, "found", '\n')
WriteToCsv(outputHomoloGene, resultingSymblList, 'a')
break
# else:
# # print(query, "is not core Nucleolar Protein.", '\n')
def UniProtRetrieve(inputHomoloGeneFile):
# print(lst)
#Use Pandas to Open and store Organism+Protein for each column
columnList = ["Human", "Cow", "Mouse", "Zebrafish", "Fly", "Worm", "Yeast", "Frog"]
df = pd.read_csv(inputHomoloGeneFile, usecols=columnList)
#Filter our terms that contain LOC
for name in columnList:
df = df[~df[name].str.contains("LOC")]
#List for Pandas
humanProteinList = df["Human"].tolist()
cowProteinList = df["Cow"].tolist()
mouseProteinList = df["Mouse"].tolist()
zebraFishProteinList = df["Zebrafish"].tolist()
flyProteinList = df["Fly"].tolist()
wormProteinListRevised = []
wormProteinList = df["Worm"].tolist()
for prot in wormProteinList:
protRevised = prot.replace('_', '-')
wormProteinListRevised.append(protRevised)
yeastProteinList = df["Yeast"].tolist()
frogProteinList = df["Frog"].tolist()
compiledProteinList = list(zip(humanProteinList,cowProteinList,mouseProteinList, zebraFishProteinList,
flyProteinList, wormProteinListRevised,yeastProteinList, frogProteinList))
#Queries Uniprot for Excel Files, and store them as temp files
##########CHANGE################
failedLst = []
for element, lst in enumerate(compiledProteinList[0:]):
for num, query in enumerate(lst):
print("Querying...", query, "for", organismNameDict[num+1])
url = "https://www.uniprot.org/uniprot/?query={query}+AND+organism:{organism}&columns=id," \
"genes(PREFERRED),organism&format=xls".format(query=query,
organism=organismTaxIDDict_Revised[num + 1])
print(url)
#Tries to write 5 times if there is an error
loopNum = 0
while loopNum < 5:
try:
r = requests.get(url, stream=False, allow_redirects=True)
tempFile = "{temp}{protNum}_{query}_{organism}.xlsx".format(temp=tempPath, protNum=(element+1), query=query,
organism=organismTaxIDDict_Revised[num + 1])
open(tempFile, 'wb').write(r.content)
print("Writing file...")
sizeFile = os.path.getsize(tempFile)
if sizeFile <=0:
try:
print("Checking File size", sizeFile)
os.remove(tempFile)
failedLst.append(query)
failedLst.append(organismTaxIDNameDict[num+1])
WriteToCsv(failedFile,failedLst,'a')
print("Failed: ", query, "for", organismTaxIDNameDict[num+1])
except:
print("No File to delete.", '\n')
break
else:
break
except:
print("Failed Writing ", query, "for", organismNameDict[num+1],"; ","Loop: ", loopNum)
loopNum +=1
if loopNum == 2:
failedLst.append(query)
failedLst.append(organismNameDict[num + 1])
WriteToCsv(failedFile, failedLst, 'a')
def UniProtIDChecker():
def all_same(items):
return all(x == items[0] for x in items)
tempPathList = []
for f in os.listdir(tempPath):
tempPathList.append(f)
tempPathListStr = [str(i) for i in tempPathList]
tempPathListStr.sort()
#print(tempPathListStr)
#Check protein name and retrieve the UniProtID
#print("ID Protein Organism")
for items in tempPathListStr:
#Opens the Files
columnList = ['Entry', 'Gene names (primary )', 'Organism']
dfProtCheck = pd.read_excel("{temp}{item}".format(temp=tempPath,item=items), usecols=columnList)
dfProtCheck.rename(columns={'Gene names (primary )': 'Gene'}, inplace=True)
dfentryList = dfProtCheck['Entry'].tolist()
dfgeneList = dfProtCheck['Gene'].tolist()
#Obtaining the Query Protein from the name of file
protName, idNum = SplitListEntry(items)
# print(idNum)
# print("ProtName: ", protName)
# print("Entry List: ", dfentryList)
# print("List: ", dfgeneList)
finalList = []
failedList = []
if len(dfgeneList) <= 2:
print(dfentryList[0]," ", protName," ", organismTaxIDNameDict[int(idNum)])
print('\n')
finalList.append(dfentryList[0])
finalList.append(protName)
finalList.append(organismTaxIDNameDict[int(idNum)])
WriteToCsv(resultFile, finalList, 'a')
elif protName in dfgeneList:
positionNum = dfgeneList.index(protName)
print(dfentryList[positionNum]," ", protName," ", organismTaxIDNameDict[int(idNum)])
print('\n')
finalList.append(dfentryList[positionNum])
finalList.append(protName)
finalList.append(organismTaxIDNameDict[int(idNum)])
WriteToCsv(resultFile, finalList, 'a')
elif all_same(dfgeneList) == True:
print(dfentryList[0], " ", protName, " ", organismTaxIDNameDict[int(idNum)])
print('\n')
finalList.append(dfentryList[0])
finalList.append(protName)
finalList.append(organismTaxIDNameDict[int(idNum)])
WriteToCsv(resultFile, finalList, 'a')
#print(protName, "appended to list")
#Might need to add elif statement for length here, or splitting characters
else:
print(protName, " NOT FOUND!", organismTaxIDNameDict[int(idNum)])
print('\n')
failedList.append(protName)
failedList.append(organismTaxIDNameDict[int(idNum)])
WriteToCsv(failedFile, failedList, 'a')
# if len(dfGeneList) == 1 or j[0:2] == query[0:2]
##----------- Execution ----------- ##
##Remove Files if they exist:
try:
files = [outputHomoloGene, resultFile, failedFile]
RemoveExistingFiles(files)
except:
print("No Existing files exist to delete.")
WriteToCsv(outputHomoloGene, ["Human", "Cow", "Mouse", "Zebrafish", "Fly", "Worm", "Yeast", "Frog"], 'a')
WriteToCsv(resultFile, ["Entry", "Gene", "Organism"], 'a')
WriteToCsv(failedFile, ["Gene", "Organism"], 'a')
try:
geneList = OpenCSV(inputFile)
HomoloGeneCheck(geneList)
UniProtRetrieve(outputHomoloGene)
UniProtIDChecker()
shutil.rmtree(tempPath)
except:
print("Failed")