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Polaris Pt 12 Point 13.txt
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Polaris Pt 12 Point 13.txt
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[2023-4-26, 14:35:13]
ImageJ2 v1.53t99
CU-IScore v1.00 (2023-04-26)
CU-MacroLibrary v1.00 (2022-07-13)
*** Scoring file ***
C:\Users\HIMSR staff\Desktop\Chris\CU-IScore (test)\Polaris Pt 12 Point 13.tif
Warning: Data has too many channels for Default color mode
1.) CK (Opal 780)
2.) Ki67 (Opal 690)
3.) CD8 (Opal 650)
4.) IER3 (Opal 620)
5.) pSTAT3 (Opal 570)
6.) CD3 (Opal 540)
7.) CD68 (Opal 520)
8.) CD19 (Opal 480)
9.) DAPI (DAPI)
10.) Autofluorescence
Bit depth: 32
Date: 2020:03:19 20:51:55
Fixed ranges: 0
Global ranges: 1
Scoring method: I-Score
Channel: CK (Opal 780)
Value range: 2.0266
Histogram bins: 2028
Pixels (total): 1270080 (1270080)
Background [0-(0.5066)]: 1004104
Low intensity [0.5066-(1.0133)]: 260606
Normal intensity [1.0133-(1.5199)]: 5326
High intensity [1.5199-2.0266]: 44
Outliers [<0, >2.0266]: 0
I-Score: 102.0355
Channel: Ki67 (Opal 690)
Value range: 13.6691
Histogram bins: 13670
Pixels (total): 1270080 (1270080)
Background [0-(3.4173)]: 1236430
Low intensity [3.4173-(6.8345)]: 29925
Normal intensity [6.8345-(10.2518)]: 3605
High intensity [10.2518-13.6691]: 120
Outliers [<0, >13.6691]: 0
I-Score: 111.4264
Channel: CD8 (Opal 650)
Value range: 19.7021
Histogram bins: 19702
Pixels (total): 1270080 (1270080)
Background [0-(4.9255)]: 1261903
Low intensity [4.9255-(9.8511)]: 6926
Normal intensity [9.8511-(14.7766)]: 1117
High intensity [14.7766-19.7021]: 134
Outliers [<0, >19.7021]: 0
I-Score: 116.9378
Channel: IER3 (Opal 620)
Value range: 2.3453
Histogram bins: 2346
Pixels (total): 1270080 (1270080)
Background [0-(0.5863)]: 1269595
Low intensity [0.5863-(1.1726)]: 385
Normal intensity [1.1726-(1.759)]: 93
High intensity [1.759-2.3453]: 7
Outliers [<0, >2.3453]: 0
I-Score: 122.0619
Channel: pSTAT3 (Opal 570)
Value range: 6.4709
Histogram bins: 6472
Pixels (total): 1270080 (1270080)
Background [0-(1.6177)]: 1269204
Low intensity [1.6177-(3.2354)]: 767
Normal intensity [3.2354-(4.8532)]: 93
High intensity [4.8532-6.4709]: 16
Outliers [<0, >6.4709]: 0
I-Score: 114.2694
Channel: CD3 (Opal 540)
Value range: 8.4783
Histogram bins: 8478
Pixels (total): 1270080 (1270080)
Background [0-(2.1196)]: 1267932
Low intensity [2.1196-(4.2392)]: 1943
Normal intensity [4.2392-(6.3587)]: 183
High intensity [6.3587-8.4783]: 22
Outliers [<0, >8.4783]: 0
I-Score: 110.568
Channel: CD68 (Opal 520)
Value range: 47.8135
Histogram bins: 47814
Pixels (total): 1270080 (1270080)
Background [0-(11.9534)]: 1251118
Low intensity [11.9534-(23.9067)]: 15634
Normal intensity [23.9067-(35.8601)]: 3055
High intensity [35.8601-47.8135]: 273
Outliers [<0, >47.8135]: 0
I-Score: 118.9906
Channel: CD19 (Opal 480)
Value range: 4.2112
Histogram bins: 4212
Pixels (total): 1270080 (1270080)
Background [0-(1.0528)]: 1268829
Low intensity [1.0528-(2.1056)]: 1130
Normal intensity [2.1056-(3.1584)]: 111
High intensity [3.1584-4.2112]: 10
Outliers [<0, >4.2112]: 0
I-Score: 110.4716
Channel: DAPI (DAPI)
Value range: 39.2338
Histogram bins: 39234
Pixels (total): 1270080 (1270080)
Background [0-(9.8085)]: 1204466
Low intensity [9.8085-(19.6169)]: 63918
Normal intensity [19.6169-(29.4254)]: 1616
High intensity [29.4254-39.2338]: 80
Outliers [<0, >39.2338]: 0
I-Score: 102.7067
Channel: Autofluorescence
Value range: 35.1712
Histogram bins: 35172
Pixels (total): 1270080 (1270080)
Background [0.01004-(8.8028)]: 1261898
Low intensity [8.8028-(17.5956)]: 5784
Normal intensity [17.5956-(26.3884)]: 2046
High intensity [26.3884-35.1812]: 352
Outliers [<0.01004, >35.1812]: 0
I-Score: 133.6104
I-Score: 114.3078 (channel average)
*** Saving result to file ***
Writing: C:/Users/HIMSR staff/Desktop/Chris/CU-IScore (test)/\Polaris Pt 12 Point 13\scores\Polaris Pt 12 Point 13.txt
[2023-4-26, 14:35:16]