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Fasta file error only when adding --ss and --exon inputs #37
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Hi @ewallace, Sorry for the late response. Would you like to send me the files so that I can reproduce and fix the issue? |
I released a new version of HISAT2 (2.0.4) three days ago, which may include some fixes for your issue. I'll close this thread and reopen if this issue still occurs. |
In 2.0.4, I also get into the same trouble when I build index for Nicotiana benthamiana.
my cluster have 500G memory, the hsat2-build would occupy up to 400G after warning up with "is not reverse-deterministic, so reverse-determinize"
the first command which only build *.3.ht2 and *.4.ht2 is fine, but the second command also can't work, this is the error :
|
I still get the same error with HISAT2 2.2.1 . Is there any reason or a workaround for this? |
Hi,
When I run
hisat2-build -p 4 ${genome_file} ${index_file}
the index builds fine.
However, when I use the same inputs but run:
hisat2-build -p 4 --ss ${ss_file} --exon ${exon_file} ${genome_file} ${index_file}
Then I get the error:
Now, this is clearly not an accurate error report as it's the same fasta file.
I'm happy to supply the input file if you want (this is yeast so takes ~1min to build index).
Thanks
Edward
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