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RDamIDSeq

All in R DamID sequencing pipeline.
Includes adapter removal, Mapping and enrichment finding for DamID-Seq reads.

For more information see also: Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development

install

  1. Install R (only 64 bit version!)
    R Project

  2. Download DamIDseq_0.1.3.tar.gz

  3. install additional packages needed for the pipeline in R

install.packages(c('corrplot', 'aqfig'))
source("https://bioconductor.org/biocLite.R")
biocLite()
biocLite(c("QuasR", "ShortRead", "GenomicRanges", "S4Vectors", "IRanges",
"BiocInstaller", "Biobase", "Biostrings", "BSgenome", "GenomicFeatures",
"GenomicAlignments", "BiocParallel", "GenomeInfoDb", "rtracklayer",
"GenomicFiles", "Rbowtie"))

  1. install DamIDSeq in R
    (exchange PATH with the path to the installation file on your disk)

install.packages("PATH/DamIDseq_0.1.3.tar.gz", repos = NULL, type = "source")

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All in R DamID sequencing pipeline

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