All in R DamID sequencing pipeline.
Includes adapter removal, Mapping and enrichment finding for DamID-Seq reads.
For more information see also: Tools for DNA adenine methyltransferase identification analysis of nuclear organization during C. elegans development
-
Install R (only 64 bit version!)
R Project -
Download DamIDseq_0.1.3.tar.gz
-
install additional packages needed for the pipeline in R
install.packages(c('corrplot', 'aqfig'))
source("https://bioconductor.org/biocLite.R")
biocLite()
biocLite(c("QuasR", "ShortRead", "GenomicRanges", "S4Vectors", "IRanges",
"BiocInstaller", "Biobase", "Biostrings", "BSgenome", "GenomicFeatures",
"GenomicAlignments", "BiocParallel", "GenomeInfoDb", "rtracklayer",
"GenomicFiles", "Rbowtie"))
- install DamIDSeq in R
(exchangePATH
with the path to the installation file on your disk)
install.packages("PATH/DamIDseq_0.1.3.tar.gz", repos = NULL, type = "source")