Six-frame Genome-Inferred Libraries for LC-MS/MS
Sixgill is a tool for using shotgun metagenomics sequencing reads to construct databases of 'metapeptides': short protein fragments for database search of LC-MS/MS metaproteomics data. A comprehensive set of resources for building and using metapeptide databases is here.
Sixgill is also available through a Galaxy interface.
Sixgill supports Python 2.7
Sixgill uses the following python packages:
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Get pip, if you don’t have it yet.
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Install Sixgill and its dependencies:
pip install biopython pysam sixgill
The Sixgill wiki contains more detailed information on using Sixgill.