Six-frame Genome-Inferred Libraries for LC-MS/MS
What is Sixgill?
Sixgill is a tool for using shotgun metagenomics sequencing reads to construct databases of 'metapeptides': short protein fragments for database search of LC-MS/MS metaproteomics data. A comprehensive set of resources for building and using metapeptide databases is here.
Sixgill is also available through a Galaxy interface.
Required Python versions
Sixgill supports Python 2.7
Sixgill uses the following python packages:
Get pip, if you don’t have it yet.
Install Sixgill and its dependencies:
pip install biopython pysam sixgill
The Sixgill wiki contains more detailed information on using Sixgill.