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Annotation format requirement #5
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Hi Shams, my apologies for the late response. Yes, you can use an entire pathway database like the C2 bundle from MSigDB. The important thing is you format the gene set annotation dictionary correctly. The dictionary has to include all cell types from your
Having said that, we believe that best results can be obtained by limiting the number of gene sets to coherent interpretable genes of similar size and with limited redundancy (please see the manuscript Supplementary Methods for further detail https://doi.org/10.1101/2022.12.20.521311 ). We also offer a package to select gene sets for Spectra which we will update with an extended set of annotations (including cancer cell and stroma cell gene sets) in the near future https://github.com/wallet-maker/cytopus . Let me know if that helps |
Thanks for the reply. Is there an example code snippet format the jason file from MSiGDB? |
Hi Shams, https://github.com/dpeerlab/spectra/blob/main/notebooks/example_notebook.ipynb Thank you, |
What annotation format is required? Is it possible to use the gene sets directly from the pathway database? for instances the C2
jason
bundle from the Broad Institute pathway database?Thanks,
Shams
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