R scripts for analyzing detection and quantification results of dengue virus (DENV) from one-step reverse-transcription droplet digital PCR (RT-ddPCR).

This repository contains R scripts used for generating results and figures in the manuscript, "Application of One-Step Reverse Transcription-Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens" by Mairiang D et al.

SenseSpec.R

This script is used for determining the lower limit of detection (LLOD95) and the lower limit of quantifiction (LLOQ) of RT-ddPCR in measuring DENV. Figure 3 was produced by this script.

Intralab_qRTPCR_v_ddPCR.R

This script is used for evaluating the DENV quantification agreement between qRT-PCR and RT-ddPCR. Figure 4, Figure S2 and Figure S3 were produced by this script.

Interlab_ddPCR.R

This script is used for evaluating the DENV quantification agreement of RT-ddPCR performed independently in three laboratories using the identical protocol. Figure 5 and Figure 6 were produced by this script.

Interlab_qRTPCR.R

This script is used for evaluating the DENV quantification agreement of qRT-PCR performed independently in two laboratories using two different protocols. Figure S4 was produced by this script.

ViralLoad_v_Severity.R

This script is used for evaluate the correlation between DENV levels and disease severity. Figure S5 was produced by this script.