R scripts for analyzing detection and quantification results of dengue virus (DENV) from one-step reverse-transcription droplet digital PCR (RT-ddPCR).
This repository contains R scripts used for generating results and figures in the manuscript, "Application of One-Step Reverse Transcription-Droplet Digital PCR for Dengue Virus Detection and Quantification in Clinical Specimens" by Mairiang D et al.
This script is used for determining the lower limit of detection (LLOD95) and the lower limit of quantifiction (LLOQ) of RT-ddPCR in measuring DENV. Figure 3 was produced by this script.
This script is used for evaluating the DENV quantification agreement between qRT-PCR and RT-ddPCR. Figure 4, Figure S2 and Figure S3 were produced by this script.
This script is used for evaluating the DENV quantification agreement of RT-ddPCR performed independently in three laboratories using the identical protocol. Figure 5 and Figure 6 were produced by this script.
This script is used for evaluating the DENV quantification agreement of qRT-PCR performed independently in two laboratories using two different protocols. Figure S4 was produced by this script.
This script is used for evaluate the correlation between DENV levels and disease severity. Figure S5 was produced by this script.