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Trim, Align to genome, Deduplicate, Realign WGS sequencing samples

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WGS data preparation

Pipeline to clean, align, and prepare sequence data for WGS analyses

WARNING

These scripts work only for members of the Bernatchez lab on the katak and manitou servers. They will require modifications and software installation to work under other circumstances.

Attribution

  • Scripts 03 to 08 are based on scripts by Clément Rougeux
  • Script 09 is based on a script by Nina Overgaard Therkildsen

Usage

Run each script in 01_scripts individually or adjust the SLURM job values in runall.sh and launch:

./runall.sh

Licence

MIT License

Copyright (c) 2018 Eric Normandeau

Permission is hereby granted, free of charge, to any person obtaining a copy of this software and associated documentation files (the "Software"), to deal in the Software without restriction, including without limitation the rights to use, copy, modify, merge, publish, distribute, sublicense, and/or sell copies of the Software, and to permit persons to whom the Software is furnished to do so, subject to the following conditions:

The above copyright notice and this permission notice shall be included in all copies or substantial portions of the Software.

THE SOFTWARE IS PROVIDED "AS IS", WITHOUT WARRANTY OF ANY KIND, EXPRESS OR IMPLIED, INCLUDING BUT NOT LIMITED TO THE WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE AND NONINFRINGEMENT. IN NO EVENT SHALL THE AUTHORS OR COPYRIGHT HOLDERS BE LIABLE FOR ANY CLAIM, DAMAGES OR OTHER LIABILITY, WHETHER IN AN ACTION OF CONTRACT, TORT OR OTHERWISE, ARISING FROM, OUT OF OR IN CONNECTION WITH THE SOFTWARE OR THE USE OR OTHER DEALINGS IN THE SOFTWARE.

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