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.../tutorials/scrna-data-ingest/workflows/AnnData-to-Cell-Data-Set-(CDS)-conversion-test.yml
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- doc: Test outline for AnnData-to-Cell-Data-Set-(CDS)-conversion | ||
job: | ||
AnnData: | ||
class: File | ||
path: test-data/AnnData.h5ad | ||
filetype: h5ad | ||
outputs: | ||
'Inspect AnnData: var': | ||
path: 'test-data/Inspect AnnData: var.tabular' | ||
CDS Monocle file: | ||
path: test-data/CDS Monocle file.rdata | ||
'Inspect AnnData: obs': | ||
path: 'test-data/Inspect AnnData: obs.tabular' | ||
'Inspect AnnData: X': | ||
path: 'test-data/Inspect AnnData: X.tabular' |
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...e-cell/tutorials/scrna-data-ingest/workflows/AnnData-to-Cell-Data-Set-(CDS)-conversion.ga
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{"a_galaxy_workflow": "true", "annotation": "AnnData to CDS format conversion (manually using Galaxy buttons). This workflow does not include renaming the column containing gene symbols. ", "creator": [{"class": "Person", "identifier": "0009-0001-2017-8805", "name": "Julia Jakiela"}], "format-version": "0.1", "license": "CC-BY-4.0", "name": "AnnData to Cell Data Set (CDS) conversion", "steps": {"0": {"annotation": "Input file in AnnData format", "content_id": null, "errors": null, "id": 0, "input_connections": {}, "inputs": [{"description": "Input file in AnnData format", "name": "AnnData"}], "label": "AnnData", "name": "Input dataset", "outputs": [], "position": {"left": 0, "top": 0}, "tool_id": null, "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "803b5429-24a8-4ba8-b9f0-d9012a23d190", "when": null, "workflow_outputs": []}, "1": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "errors": null, "id": 1, "input_connections": {"input": {"id": 0, "output_name": "output"}}, "inputs": [], "label": null, "name": "Inspect AnnData", "outputs": [{"name": "obs", "type": "tabular"}], "position": {"left": 256.73787564688695, "top": 79.01355786263034}, "post_job_actions": {"RenameDatasetActionobs": {"action_arguments": {"newname": "Cell barcodes"}, "action_type": "RenameDatasetAction", "output_name": "obs"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "tool_shed_repository": {"changeset_revision": "ee98d611afc6", "name": "anndata_inspect", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"h5ad\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inspect\": {\"info\": \"obs\", \"__current_case__\": 3}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.7.5+galaxy1", "type": "tool", "uuid": "3dd0c427-0689-47b4-bfe4-1ad3127972d8", "when": null, "workflow_outputs": [{"label": "Inspect AnnData: obs", "output_name": "obs", "uuid": "7bdb825c-6a89-4a34-bbd9-a79c309c9750"}]}, "2": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "errors": null, "id": 2, "input_connections": {"input": {"id": 0, "output_name": "output"}}, "inputs": [], "label": null, "name": "Inspect AnnData", "outputs": [{"name": "var", "type": "tabular"}], "position": {"left": 298.0404350277319, "top": 221.00360766192858}, "post_job_actions": {"RenameDatasetActionvar": {"action_arguments": {"newname": "Genes"}, "action_type": "RenameDatasetAction", "output_name": "var"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "tool_shed_repository": {"changeset_revision": "ee98d611afc6", "name": "anndata_inspect", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"h5ad\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inspect\": {\"info\": \"var\", \"__current_case__\": 4}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.7.5+galaxy1", "type": "tool", "uuid": "dd941b59-7d88-4f2a-8241-9ce658bab555", "when": null, "workflow_outputs": [{"label": "Inspect AnnData: var", "output_name": "var", "uuid": "79a2e3e5-4bd6-42de-864c-a5dc2412cb86"}]}, "3": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "errors": null, "id": 3, "input_connections": {"input": {"id": 0, "output_name": "output"}}, "inputs": [], "label": null, "name": "Inspect AnnData", "outputs": [{"name": "X", "type": "tabular"}], "position": {"left": 362.5640208496929, "top": 368.5465046492902}, "post_job_actions": {"RenameDatasetActionX": {"action_arguments": {"newname": "Expression matrix"}, "action_type": "RenameDatasetAction", "output_name": "X"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.7.5+galaxy1", "tool_shed_repository": {"changeset_revision": "ee98d611afc6", "name": "anndata_inspect", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"h5ad\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inspect\": {\"info\": \"X\", \"__current_case__\": 1}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.7.5+galaxy1", "type": "tool", "uuid": "535c49c0-591e-47b6-8816-20ee58cd7e37", "when": null, "workflow_outputs": [{"label": "Inspect AnnData: X", "output_name": "X", "uuid": "7a254954-7e32-4e8a-bd4c-a79abf9db8d8"}]}, "4": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_transpose/datamash_transpose/1.8+galaxy1", "errors": null, "id": 4, "input_connections": {"in_file": {"id": 3, "output_name": "X"}}, "inputs": [], "label": null, "name": "Transpose", "outputs": [{"name": "out_file", "type": "input"}], "position": {"left": 599.440446949216, "top": 465.45422444853756}, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_transpose/datamash_transpose/1.8+galaxy1", "tool_shed_repository": {"changeset_revision": "a513e3fbb4c5", "name": "datamash_transpose", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"input\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"in_file\": {\"__class__\": \"ConnectedValue\"}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.8+galaxy1", "type": "tool", "uuid": "058abe52-0702-4ef2-a770-bb0a57d69773", "when": null, "workflow_outputs": []}, "5": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/ebi-gxa/monocle3_create/monocle3_create/0.1.4+galaxy2", "errors": null, "id": 5, "input_connections": {"cell_metadata": {"id": 1, "output_name": "obs"}, "expression_matrix": {"id": 4, "output_name": "out_file"}, "gene_annotation": {"id": 2, "output_name": "var"}}, "inputs": [], "label": null, "name": "Monocle3 create", "outputs": [{"name": "output_rds", "type": "rdata"}], "position": {"left": 848.1105796111376, "top": 495.7890097555672}, "post_job_actions": {"RenameDatasetActionoutput_rds": {"action_arguments": {"newname": "CDS Monocle file"}, "action_type": "RenameDatasetAction", "output_name": "output_rds"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/ebi-gxa/monocle3_create/monocle3_create/0.1.4+galaxy2", "tool_shed_repository": {"changeset_revision": "ada68a9d0aec", "name": "monocle3_create", "owner": "ebi-gxa", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"input\", \"cell_metadata\": {\"__class__\": \"ConnectedValue\"}, \"cell_metadata_format\": \"tsv\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"expression_matrix\": {\"__class__\": \"ConnectedValue\"}, \"expression_matrix_format\": \"tsv\", \"gene_annotation\": {\"__class__\": \"ConnectedValue\"}, \"gene_annotation_format\": \"tsv\", \"introspective\": true, \"output_object_format\": \"cds3\", \"verbose\": false, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.1.4+galaxy2", "type": "tool", "uuid": "17a4bbe9-5a3c-4c54-b581-c24588ccf397", "when": null, "workflow_outputs": [{"label": "CDS Monocle file", "output_name": "output_rds", "uuid": "6bf4a0ea-3cb9-470c-9863-0a7eb0bc3d8c"}]}}, "tags": [], "uuid": "d0ce033d-5669-475c-879b-ce5391b029aa", "version": 7} |
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...s/single-cell/tutorials/scrna-data-ingest/workflows/AnnData-to-Seurat-conversion-test.yml
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- doc: Test outline for AnnData-to-Seurat-conversion | ||
job: | ||
AnnData: | ||
class: File | ||
path: test-data/AnnData.h5ad | ||
filetype: h5ad | ||
outputs: | ||
'Inspect AnnData: X': | ||
path: 'test-data/Inspect AnnData: X.tabular' | ||
Converted Seurat object: | ||
path: test-data/Converted Seurat object.rdata | ||
'Inspect AnnData: obs': | ||
path: 'test-data/Inspect AnnData: obs.tabular' |
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topics/single-cell/tutorials/scrna-data-ingest/workflows/AnnData-to-Seurat-conversion.ga
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{"a_galaxy_workflow": "true", "annotation": "AnnData to Seurat format conversion (manually using Galaxy buttons)", "creator": [{"class": "Person", "identifier": "0009-0001-2017-8805", "name": "Julia Jakiela"}, {"class": "Person", "identifier": "0009-0008-9422-6380", "name": "Morgan Howells"}], "format-version": "0.1", "license": "CC-BY-4.0", "name": "AnnData to Seurat conversion", "steps": {"0": {"annotation": "Input file in AnnData format", "content_id": null, "errors": null, "id": 0, "input_connections": {}, "inputs": [{"description": "Input file in AnnData format", "name": "AnnData"}], "label": "AnnData", "name": "Input dataset", "outputs": [], "position": {"left": 0, "top": 0}, "tool_id": null, "tool_state": "{\"optional\": false, \"tag\": null}", "tool_version": null, "type": "data_input", "uuid": "f783e6c3-808c-4dd2-84dd-6130c8230970", "when": null, "workflow_outputs": []}, "1": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0", "errors": null, "id": 1, "input_connections": {"input": {"id": 0, "output_name": "output"}}, "inputs": [], "label": null, "name": "Inspect AnnData", "outputs": [{"name": "obs", "type": "tabular"}], "position": {"left": 242.47774426074216, "top": 60.81245422363281}, "post_job_actions": {"RenameDatasetActionobs": {"action_arguments": {"newname": "Observations"}, "action_type": "RenameDatasetAction", "output_name": "obs"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0", "tool_shed_repository": {"changeset_revision": "6f0d0c784f09", "name": "anndata_inspect", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"h5ad\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inspect\": {\"info\": \"obs\", \"__current_case__\": 3}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.10.3+galaxy0", "type": "tool", "uuid": "202fdb30-8c0e-4a43-9909-2fcc051a4331", "when": null, "workflow_outputs": [{"label": "Inspect AnnData: obs", "output_name": "obs", "uuid": "6d03d713-8d71-4f6a-9f83-cd999f423ebf"}]}, "2": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0", "errors": null, "id": 2, "input_connections": {"input": {"id": 0, "output_name": "output"}}, "inputs": [], "label": null, "name": "Inspect AnnData", "outputs": [{"name": "X", "type": "tabular"}], "position": {"left": 276.0902404785156, "top": 273.5749816894531}, "post_job_actions": {"RenameDatasetActionX": {"action_arguments": {"newname": "Matrix"}, "action_type": "RenameDatasetAction", "output_name": "X"}}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/anndata_inspect/anndata_inspect/0.10.3+galaxy0", "tool_shed_repository": {"changeset_revision": "6f0d0c784f09", "name": "anndata_inspect", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"__input_ext\": \"h5ad\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"input\": {\"__class__\": \"ConnectedValue\"}, \"inspect\": {\"info\": \"X\", \"__current_case__\": 1}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "0.10.3+galaxy0", "type": "tool", "uuid": "2d2961d5-fe61-4c57-af14-cad6f77e8ebc", "when": null, "workflow_outputs": [{"label": "Inspect AnnData: X", "output_name": "X", "uuid": "ea025a8a-6f3a-4e38-a3af-db406886c7de"}]}, "3": {"annotation": "", "content_id": "toolshed.g2.bx.psu.edu/repos/iuc/datamash_transpose/datamash_transpose/1.8+galaxy1", "errors": null, "id": 3, "input_connections": {"in_file": {"id": 2, "output_name": "X"}}, "inputs": [], "label": null, "name": "Transpose", "outputs": [{"name": "out_file", "type": "input"}], "position": {"left": 524.1027526855469, "top": 414.38751220703125}, "post_job_actions": {}, "tool_id": 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{"name": "genes_out", "type": "tsv"}, {"name": "matrix_out", "type": "mtx"}], "position": {"left": 776.1026916503906, "top": 497.0278162336424}, "post_job_actions": {}, "tool_id": "toolshed.g2.bx.psu.edu/repos/iuc/dropletutils/dropletutils/1.10.0+galaxy2", "tool_shed_repository": {"changeset_revision": "a9caad671439", "name": "dropletutils", "owner": "iuc", "tool_shed": "toolshed.g2.bx.psu.edu"}, "tool_state": "{\"operation\": {\"use\": \"filter\", \"__current_case__\": 0, \"method\": {\"use\": \"defaultdrops\", \"__current_case__\": 0, \"expected\": \"338\", \"upperquant\": \"1.0\", \"lowerprop\": \"0.0\"}, \"outformat\": \"directory\"}, \"seed\": \"100\", \"tenx_format\": {\"use\": \"tsv\", \"__current_case__\": 2, \"input\": {\"__class__\": \"RuntimeValue\"}}, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "1.10.0+galaxy2", "type": "tool", "uuid": "f894f102-5f72-4323-a6c0-d389813b027a", "when": null, "workflow_outputs": []}, "5": {"annotation": "", 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"{\"__input_ext\": \"input\", \"chromInfo\": \"/opt/galaxy/tool-data/shared/ucsc/chrom/?.len\", \"format\": \"rds_seurat\", \"input\": {\"format\": \"mtx\", \"__current_case__\": 1, \"matrix\": {\"__class__\": \"ConnectedValue\"}, \"genes\": {\"__class__\": \"ConnectedValue\"}, \"barcodes\": {\"__class__\": \"ConnectedValue\"}}, \"metadata\": {\"__class__\": \"ConnectedValue\"}, \"min_cells\": null, \"min_features\": null, \"__page__\": null, \"__rerun_remap_job_id__\": null}", "tool_version": "3.2.3+galaxy0", "type": "tool", "uuid": "72c0273d-01fa-45bd-af67-3dd0ef51b761", "when": null, "workflow_outputs": [{"label": "Converted Seurat object", "output_name": "rds_seurat_file", "uuid": "e5fec5dc-4706-48e5-8326-05e70290552d"}]}}, "tags": [], "uuid": "8066a342-5980-4c4f-ab4d-7e840dd9e2bf", "version": 7} |
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...als/scrna-data-ingest/workflows/AnnData-to-SingleCellExperiment-(SCE)-conversion-test.yml
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- doc: Test outline for AnnData-to-SingleCellExperiment-(SCE)-conversion | ||
job: | ||
AnnData: | ||
class: File | ||
path: test-data/AnnData.h5ad | ||
filetype: h5ad | ||
Cell ID column: index | ||
outputs: | ||
'Inspect AnnData: obs': | ||
path: 'test-data/Inspect AnnData: obs.tabular' | ||
'Inspect AnnData: X': | ||
path: 'test-data/Inspect AnnData: X.tabular' | ||
Converted SCE object: | ||
path: test-data/Converted SCE object.rdata |
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