PTN000021568 CalpC NOT calcium-dependent cysteine-type endopeptidase activity

[hattrill]

Could you block calcium-dependent cysteine-type endopeptidase activity from PTN000021568 to FBgn0260450.

CalpC, FBgn0260450 lacks the catalytic residues for activity:
Q9VXH6 CalpC NOT|involved_in GO:0006508 proteolysis ECO:0000320 IKR PMID:12646209 20121101

[thomaspd]

@marcfeuermann , can you please see where these catalytic residues were lost in the tree for PTHR10183, and make the corresponding IKR loss for whichever functions were lost there?

[pgaudet]

@thomaspd @muggity

We used to take into account the NOT annotations and not propagate contradictory positive annotations. Perhaps we should also take into account IRK annotations (some of these are made manually, not by PAINT).

What do you think ?

Thanks, Pascale

[marcfeuermann]

Family locked by Pascale. @pgaudet are you reviewing this one or should I do it ?

[marcfeuermann]

Very good point. I think we indeed should take into account IRK annotations (I thought it was already the case). For PTHR10183, I've checked the alignment for the presence of the 3 active sites and blocked propagation when at least one of them was absent. @pgaudet , do you want us to keep this issue open for further discussion or should we close it ?

[pgaudet]

I dont have PTHR10183 locked !! Very strange... Can you check again ?

[pgaudet]

Never mind ! That was a while ago.

@marcfeuermann If you blocked the annotations that's OK. In these cases I would use IRK, not just 'block' propagation (IRD).

[marcfeuermann]

It was not locked anymore when I made the corrections. I indeed used IRK, not IRD :-)