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somatic_exome_cle.cwl
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somatic_exome_cle.cwl
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#!/usr/bin/env cwl-runner
cwlVersion: v1.0
class: Workflow
label: "exome alignment and somatic variant detection for cle purpose"
requirements:
- class: SchemaDefRequirement
types:
- $import: ../types/labelled_file.yml
- $import: ../types/sequence_data.yml
- $import: ../types/vep_custom_annotation.yml
- class: SubworkflowFeatureRequirement
- class: StepInputExpressionRequirement
inputs:
reference: string
tumor_sequence:
type: ../types/sequence_data.yml#sequence_data[]
tumor_name:
type: string?
default: 'tumor'
normal_sequence:
type: ../types/sequence_data.yml#sequence_data[]
normal_name:
type: string?
default: 'normal'
mills:
type: File
secondaryFiles: [.tbi]
known_indels:
type: File
secondaryFiles: [.tbi]
dbsnp_vcf:
type: File
secondaryFiles: [.tbi]
bqsr_intervals:
type: string[]
bait_intervals:
type: File
target_intervals:
type: File
per_base_intervals:
type: ../types/labelled_file.yml#labelled_file[]
per_target_intervals:
type: ../types/labelled_file.yml#labelled_file[]
summary_intervals:
type: ../types/labelled_file.yml#labelled_file[]
omni_vcf:
type: File
secondaryFiles: [.tbi]
picard_metric_accumulation_level:
type: string
qc_minimum_mapping_quality:
type: int?
default: 0
qc_minimum_base_quality:
type: int?
default: 0
interval_list:
type: File
strelka_cpu_reserved:
type: int?
default: 8
mutect_scatter_count:
type: int
varscan_strand_filter:
type: int?
default: 0
varscan_min_coverage:
type: int?
default: 8
varscan_min_var_freq:
type: float?
default: 0.05
varscan_p_value:
type: float?
default: 0.99
varscan_max_normal_freq:
type: float?
pindel_insert_size:
type: int
default: 400
docm_vcf:
type: File
secondaryFiles: [.tbi]
filter_docm_variants:
type: boolean?
default: true
filter_minimum_depth:
type: int?
default: 20
vep_cache_dir:
type:
- string
- Directory
vep_ensembl_assembly:
type: string
doc: "genome assembly to use in vep. Examples: GRCh38 or GRCm38"
vep_ensembl_version:
type: string
doc: "ensembl version - Must be present in the cache directory. Example: 95"
vep_ensembl_species:
type: string
doc: "ensembl species - Must be present in the cache directory. Examples: homo_sapiens or mus_musculus"
synonyms_file:
type: File?
annotate_coding_only:
type: boolean?
vep_pick:
type:
- "null"
- type: enum
symbols: ["pick", "flag_pick", "pick_allele", "per_gene", "pick_allele_gene", "flag_pick_allele", "flag_pick_allele_gene"]
cle_vcf_filter:
type: boolean
default: false
variants_to_table_fields:
type: string[]
default: [CHROM,POS,ID,REF,ALT,set,AC,AF]
variants_to_table_genotype_fields:
type: string[]
default: [GT,AD]
vep_to_table_fields:
type: string[]
default: [HGVSc,HGVSp]
vep_custom_annotations:
type: ../types/vep_custom_annotation.yml#vep_custom_annotation[]
doc: "custom type, check types directory for input format"
somalier_vcf:
type: File
disclaimer_text:
type: string?
default: "This laboratory developed test (LDT) was developed and its performance characteristics determined by the CLIA Licensed Environment laboratory at the McDonnell Genome Institute at Washington University (MGI-CLE, CLIA #26D2092546, CAP #9047655), Dr. David H. Spencer MD, PhD, FCAP, Medical Director. 4444 Forest Park Avenue, Rm 4127 St. Louis, Missouri 63108 (314) 286-1460 Fax: (314) 286-1810. The MGI-CLE laboratory is regulated under CLIA as certified to perform high-complexity testing. This test has not been cleared or approved by the FDA."
disclaimer_version:
type: string
tumor_sample_name:
type: string
normal_sample_name:
type: string
outputs:
tumor_cram:
type: File
outputSource: tumor_index_cram/indexed_cram
tumor_mark_duplicates_metrics:
type: File
outputSource: tumor_alignment_and_qc/mark_duplicates_metrics
tumor_insert_size_metrics:
type: File
outputSource: tumor_alignment_and_qc/insert_size_metrics
tumor_alignment_summary_metrics:
type: File
outputSource: tumor_alignment_and_qc/alignment_summary_metrics
tumor_hs_metrics:
type: File
outputSource: tumor_alignment_and_qc/hs_metrics
tumor_per_target_coverage_metrics:
type: File[]
outputSource: tumor_alignment_and_qc/per_target_coverage_metrics
tumor_per_target_hs_metrics:
type: File[]
outputSource: tumor_alignment_and_qc/per_target_hs_metrics
tumor_per_base_coverage_metrics:
type: File[]
outputSource: tumor_alignment_and_qc/per_base_coverage_metrics
tumor_per_base_hs_metrics:
type: File[]
outputSource: tumor_alignment_and_qc/per_base_hs_metrics
tumor_summary_hs_metrics:
type: File[]
outputSource: tumor_alignment_and_qc/summary_hs_metrics
tumor_flagstats:
type: File
outputSource: tumor_alignment_and_qc/flagstats
tumor_verify_bam_id_metrics:
type: File
outputSource: tumor_alignment_and_qc/verify_bam_id_metrics
tumor_verify_bam_id_depth:
type: File
outputSource: tumor_alignment_and_qc/verify_bam_id_depth
normal_cram:
type: File
outputSource: normal_index_cram/indexed_cram
normal_mark_duplicates_metrics:
type: File
outputSource: normal_alignment_and_qc/mark_duplicates_metrics
normal_insert_size_metrics:
type: File
outputSource: normal_alignment_and_qc/insert_size_metrics
normal_alignment_summary_metrics:
type: File
outputSource: normal_alignment_and_qc/alignment_summary_metrics
normal_hs_metrics:
type: File
outputSource: normal_alignment_and_qc/hs_metrics
normal_per_target_coverage_metrics:
type: File[]
outputSource: normal_alignment_and_qc/per_target_coverage_metrics
normal_per_target_hs_metrics:
type: File[]
outputSource: normal_alignment_and_qc/per_target_hs_metrics
normal_per_base_coverage_metrics:
type: File[]
outputSource: normal_alignment_and_qc/per_base_coverage_metrics
normal_per_base_hs_metrics:
type: File[]
outputSource: normal_alignment_and_qc/per_base_hs_metrics
normal_summary_hs_metrics:
type: File[]
outputSource: normal_alignment_and_qc/summary_hs_metrics
normal_flagstats:
type: File
outputSource: normal_alignment_and_qc/flagstats
normal_verify_bam_id_metrics:
type: File
outputSource: normal_alignment_and_qc/verify_bam_id_metrics
normal_verify_bam_id_depth:
type: File
outputSource: normal_alignment_and_qc/verify_bam_id_depth
mutect_unfiltered_vcf:
type: File
outputSource: detect_variants/mutect_unfiltered_vcf
secondaryFiles: [.tbi]
mutect_filtered_vcf:
type: File
outputSource: detect_variants/mutect_filtered_vcf
secondaryFiles: [.tbi]
strelka_unfiltered_vcf:
type: File
outputSource: detect_variants/strelka_unfiltered_vcf
secondaryFiles: [.tbi]
strelka_filtered_vcf:
type: File
outputSource: detect_variants/strelka_filtered_vcf
secondaryFiles: [.tbi]
varscan_unfiltered_vcf:
type: File
outputSource: detect_variants/varscan_unfiltered_vcf
secondaryFiles: [.tbi]
varscan_filtered_vcf:
type: File
outputSource: detect_variants/varscan_filtered_vcf
secondaryFiles: [.tbi]
pindel_unfiltered_vcf:
type: File
outputSource: detect_variants/pindel_unfiltered_vcf
secondaryFiles: [.tbi]
pindel_filtered_vcf:
type: File
outputSource: detect_variants/pindel_filtered_vcf
secondaryFiles: [.tbi]
docm_filtered_vcf:
type: File
outputSource: detect_variants/docm_filtered_vcf
secondaryFiles: [.tbi]
final_vcf:
type: File
outputSource: detect_variants/final_vcf
secondaryFiles: [.tbi]
final_filtered_vcf:
type: File
outputSource: annotated_filter_vcf_index/indexed_vcf
secondaryFiles: [.tbi]
final_tsv:
type: File
outputSource: add_disclaimer_version_to_final_tsv/output_file
vep_summary:
type: File
outputSource: detect_variants/vep_summary
tumor_snv_bam_readcount_tsv:
type: File
outputSource: detect_variants/tumor_snv_bam_readcount_tsv
tumor_indel_bam_readcount_tsv:
type: File
outputSource: detect_variants/tumor_indel_bam_readcount_tsv
normal_snv_bam_readcount_tsv:
type: File
outputSource: detect_variants/normal_snv_bam_readcount_tsv
normal_indel_bam_readcount_tsv:
type: File
outputSource: detect_variants/normal_indel_bam_readcount_tsv
somalier_concordance_metrics:
type: File
outputSource: concordance/somalier_pairs
somalier_concordance_statistics:
type: File
outputSource: concordance/somalier_samples
steps:
tumor_alignment_and_qc:
run: alignment_exome.cwl
in:
reference: reference
sequence: tumor_sequence
mills: mills
known_indels: known_indels
dbsnp_vcf: dbsnp_vcf
bqsr_intervals: bqsr_intervals
bait_intervals: bait_intervals
target_intervals: target_intervals
per_base_intervals: per_base_intervals
per_target_intervals: per_target_intervals
summary_intervals: summary_intervals
omni_vcf: omni_vcf
picard_metric_accumulation_level: picard_metric_accumulation_level
minimum_mapping_quality: qc_minimum_mapping_quality
minimum_base_quality: qc_minimum_base_quality
final_name:
source: tumor_name
valueFrom: "$(self).bam"
out:
[bam, mark_duplicates_metrics, insert_size_metrics, alignment_summary_metrics, hs_metrics, per_target_coverage_metrics, per_target_hs_metrics, per_base_coverage_metrics, per_base_hs_metrics, summary_hs_metrics, flagstats, verify_bam_id_metrics, verify_bam_id_depth]
normal_alignment_and_qc:
run: alignment_exome.cwl
in:
reference: reference
sequence: normal_sequence
mills: mills
known_indels: known_indels
dbsnp_vcf: dbsnp_vcf
bqsr_intervals: bqsr_intervals
bait_intervals: bait_intervals
target_intervals: target_intervals
per_base_intervals: per_base_intervals
per_target_intervals: per_target_intervals
summary_intervals: summary_intervals
omni_vcf: omni_vcf
picard_metric_accumulation_level: picard_metric_accumulation_level
minimum_mapping_quality: qc_minimum_mapping_quality
minimum_base_quality: qc_minimum_base_quality
final_name:
source: normal_name
valueFrom: "$(self).bam"
out:
[bam, mark_duplicates_metrics, insert_size_metrics, alignment_summary_metrics, hs_metrics, per_target_coverage_metrics, per_target_hs_metrics, per_base_coverage_metrics, per_base_hs_metrics, summary_hs_metrics, flagstats, verify_bam_id_metrics, verify_bam_id_depth]
concordance:
run: ../tools/concordance.cwl
in:
reference: reference
bam_1: tumor_alignment_and_qc/bam
bam_2: normal_alignment_and_qc/bam
vcf: somalier_vcf
out:
[somalier_pairs, somalier_samples]
detect_variants:
run: detect_variants.cwl
in:
reference: reference
tumor_bam: tumor_alignment_and_qc/bam
normal_bam: normal_alignment_and_qc/bam
interval_list: interval_list
strelka_exome_mode:
default: true
strelka_cpu_reserved: strelka_cpu_reserved
mutect_scatter_count: mutect_scatter_count
varscan_strand_filter: varscan_strand_filter
varscan_min_coverage: varscan_min_coverage
varscan_min_var_freq: varscan_min_var_freq
varscan_p_value: varscan_p_value
varscan_max_normal_freq: varscan_max_normal_freq
pindel_insert_size: pindel_insert_size
docm_vcf: docm_vcf
filter_docm_variants: filter_docm_variants
filter_minimum_depth: filter_minimum_depth
vep_cache_dir: vep_cache_dir
vep_ensembl_assembly: vep_ensembl_assembly
vep_ensembl_version: vep_ensembl_version
vep_ensembl_species: vep_ensembl_species
synonyms_file: synonyms_file
annotate_coding_only: annotate_coding_only
vep_pick: vep_pick
cle_vcf_filter: cle_vcf_filter
variants_to_table_fields: variants_to_table_fields
variants_to_table_genotype_fields: variants_to_table_genotype_fields
vep_to_table_fields: vep_to_table_fields
tumor_sample_name: tumor_sample_name
normal_sample_name: normal_sample_name
vep_custom_annotations: vep_custom_annotations
out:
[mutect_unfiltered_vcf, mutect_filtered_vcf, strelka_unfiltered_vcf, strelka_filtered_vcf, varscan_unfiltered_vcf, varscan_filtered_vcf, pindel_unfiltered_vcf, pindel_filtered_vcf, docm_filtered_vcf, final_vcf, final_filtered_vcf, final_tsv, vep_summary, tumor_snv_bam_readcount_tsv, tumor_indel_bam_readcount_tsv, normal_snv_bam_readcount_tsv, normal_indel_bam_readcount_tsv]
add_disclaimer_to_final_tsv:
run: ../tools/add_string_at_line.cwl
in:
input_file: detect_variants/final_tsv
line_number:
default: 1
some_text:
source: disclaimer_text
valueFrom: "#$(self)"
output_name:
source: detect_variants/final_tsv
valueFrom: "$(self.basename)"
out:
[output_file]
add_disclaimer_version_to_final_tsv:
run: ../tools/add_string_at_line.cwl
in:
input_file: add_disclaimer_to_final_tsv/output_file
line_number:
default: 2
some_text:
source: disclaimer_version
valueFrom: "#The software version is $(self)"
output_name:
source: add_disclaimer_to_final_tsv/output_file
valueFrom: "$(self.basename)"
out:
[output_file]
add_disclaimer_to_final_filtered_vcf:
run: ../tools/add_string_at_line_bgzipped.cwl
in:
input_file: detect_variants/final_filtered_vcf
line_number:
default: 2
some_text:
source: disclaimer_text
valueFrom: "##DisclaimerText=$(self)"
output_name:
source: detect_variants/final_filtered_vcf
valueFrom: "$(self.basename)"
out:
[output_file]
add_disclaimer_version_to_final_filtered_vcf:
run: ../tools/add_string_at_line_bgzipped.cwl
in:
input_file: add_disclaimer_to_final_filtered_vcf/output_file
line_number:
default: 3
some_text:
source: disclaimer_version
valueFrom: "##CLESoftwareVersion=$(self)"
output_name:
source: add_disclaimer_to_final_filtered_vcf/output_file
valueFrom: "$(self.basename)"
out:
[output_file]
annotated_filter_vcf_index:
run: ../tools/index_vcf.cwl
in:
vcf: add_disclaimer_version_to_final_filtered_vcf/output_file
out:
[indexed_vcf]
tumor_bam_to_cram:
run: ../tools/bam_to_cram.cwl
in:
bam: tumor_alignment_and_qc/bam
reference: reference
out:
[cram]
tumor_index_cram:
run: ../tools/index_cram.cwl
in:
cram: tumor_bam_to_cram/cram
out:
[indexed_cram]
normal_bam_to_cram:
run: ../tools/bam_to_cram.cwl
in:
bam: normal_alignment_and_qc/bam
reference: reference
out:
[cram]
normal_index_cram:
run: ../tools/index_cram.cwl
in:
cram: normal_bam_to_cram/cram
out:
[indexed_cram]