no subreads available

[michaelabrouk]

Hi,

I am working with sequencing output coming from Sequel IIe meaning that I only have the ccs.bam files available and not the subreads.bam.
Is DeepConsensus can work without the alignment of subreads to ccs using actc in the preliminary step?

Thanks

[armintoepfer]

DeepConsensus requires subreads, which are not available when using Sequel IIe HiFi output.

[michaelabrouk]

Thank you for your answer.