FlowWorkspace.jl

This package provides for loading and parsing of FlowJo Workspace files in pure Julia. The gating strategy is parsed as a DiMetaGraph and group membership as Dict. Together with GigaSOM.jl this metadata can be used to generate event-level labels for FCS datasets.

Installation

Type ] add FlowWorkspace and then hit ⏎ Return at the REPL. You should see pkg> add FlowWorkspace.

Basic Usage

⚠️ FCS files under a workspace must have unique names. This limitation will be removed in future versions

The load method returns a tuple of three DataFrames and one Dict:

• data transformed fluorescence values for each event
• labels boolean telling us which events have been gated
• groups booleans telling us which group each event belongs to
• gating Dictionary of graph representations of each gating strategy

using FlowWorkspace
data,labels,groups,gating = load("workspace.wsp"; files="*.fcs", cols=:setequal)