This repository contains data and analysis code for:
"Neurotransmission-Related Gene Expression in the Frontal Pole (Brodmann Area 10) is Altered in Subjects with Bipolar Disorder and Schizophrenia" by Adriana Medina, Megan Hastings Hagenauer, David M. Krolewski, Evan Hughes, Liam Cannon Thew Forrester, David M. Walsh, Maria Waselus, Evelyn Richardson, Cortney A. Turner, Adolfo Sequeira, Preston Cartagena, Robert C. Thompson, Marquis Vawter, Blynn Bunney, Richard M. Myers, Jack D. Barchas, Francis Lee, Alan F. Schatzberg, William E. Bunney, Huda Akil, Stanley J. Watson. medRxiv 2022.06.03.22275600; doi: https://doi.org/10.1101/2022.06.03.22275600 (Preprint)
This code was primarily written by Liam Cannon Thew Forrester, with mentorship and guidance by Megan Hagenauer.
This code focuses on the re-analysis and meta-analysis of public frontal pole (BA10) microarray data to accompany our qPCR analysis of neurotransmission-related gene expression in BA10 in subjects with Bipolar Disorder and Schizophrenia.
The two public datasets used were: Gene Expression Omnibus #GSE1265420: n=50, CTRL: n=15, BPD: n=11, SCHIZ: n=13, MDD=11; Gene Expression Omnibus #GSE1761221: n=51, CTRL: n=23, SCHIZ: n=28
The code was intended for single use, and is minimally annotated and occasionally non-linear (especially in areas of troubleshooting) or completely tangential to the primary goals of the project. Feel free to message me if you have questions.
The code for the qPCR analyses is found in a different repository: https://github.com/hagenaue/Adriana_FrontalPole