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cas-offinder-bulge target sequence change #1

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Viscacha22 opened this issue Sep 19, 2018 · 1 comment
Closed

cas-offinder-bulge target sequence change #1

Viscacha22 opened this issue Sep 19, 2018 · 1 comment

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@Viscacha22
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Hi,

I ran cas-offinder-bulge and noticed that the target sequences in my output .txt file are different from my input sequences. Specifically, the first base of the target sequence is gone, and there is an "N" inserted at the end of the sequence, right before the PAM sequence. The issue is present for both RNA and DNA bulges.

input:
./Cas-OFFinder/chromosomes
NNNNNNNNNNNNNNNNNNNNNRG 1 1
CTAACAGTTGCTTTTATCACNGG 3

output (crRNA):
TAACAGTTGCTTTTATCACNNRG

I don't have this problem when I run the original cas-offinder.

Thanks!

@Viscacha22
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Thanks for uploading a new version; it runs without any issues now.

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