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Detect RNA-seq strandedness in FASTQ #92

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edsu7 opened this issue Aug 10, 2022 · 9 comments
Open

Detect RNA-seq strandedness in FASTQ #92

edsu7 opened this issue Aug 10, 2022 · 9 comments
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@edsu7
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edsu7 commented Aug 10, 2022

We need to run an alignment on our FASTQ files or a subset of them to determine the strandedness; need to add this check to our quite of seq-tools checks

@b-f-chan
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b-f-chan commented Sep 7, 2022

Suggestion: @edsu7 can you investigate Salmon to see if it can do this instead of us coding this into our scripts

https://salmon.readthedocs.io/en/latest/library_type.html#fragment-library-types

@b-f-chan
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Started this work, but encountered issues, may need to run on Linux not MacOS

@edsu7
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edsu7 commented Sep 15, 2022

Based on below, it should take ~15 minutes for 600 million reads.

Posted into the wrong ticket.
Table removed

@b-f-chan
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Had to pull public data to be used; work ongoing

@b-f-chan
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b-f-chan commented Nov 2, 2022

On hold due to other priorities

@b-f-chan
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Targeting PR for this week

Some separate investigation required for NF-Core support

@b-f-chan
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Still low priority, leaving PR open until testing can be done

@puneet-oicr
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@puneet-oicr
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