pfastq-dump

pfastq-dump is a bash implementation of parallel-fastq-dump, parallel fastq-dump wrapper. --stdout option is additionally supported, but almost same features. It also uses -N and -X options of fastq-dump to specify blocks of data to be decompressed separately.

Install

Clone this repository and change permission.

$ git clone https://github.com/inutano/pfastq-dump
$ cd pfastq-dump
$ chmod a+x bin/pfastq-dump

then copy/move to wherever you like.

Prerequisites

Install sra-tools and make sure two binaries below are in $PATH.

• fastq-dump
• sra-stat

Usage

Basic usage:

$ pfastq-dump -t <number of threads> [options] <path to .sra file> [<path> ...]

It is strongly recommended here as well that the .sra data should be downloaded via aspera-connect/ftp/prefetch command, not by fastq-dump.

$ pfastq-dump --threads 8 --outdir /path/to/outdir /path/to/SRR000001.sra

--stdout option is to send decompressed reads to stdout, but may not work in the way you expected. pfastq-dump once creates separated files on the disk, then just cat them. This feature is to be used in a workflow that requires stdout input of sequence reads, and marge multiple fastq files into one file.

$ pfastq-dump --threads 8 --stdout /path/to/**/*sra > data.fastq

Mind the disk usage as well as the original implementation warns, this script requires double of decompressed fastq file size.

Docker container

Available on Quay.io

$ ls
mydata.sra
$ docker run --rm -v "$(pwd)":/data -w /data quay.io/inutano/sra-toolkit:v2.9.2 pfastq-dump --threads 8 /data/mydata.sra

Copyright

Copyright (c) 2017 Tazro Inutano Ohta. See LICENSE.txt for further details.