Denoising (using qiime2's dada2 integration for paired / single end data.
- Demultiplexed sequences as a qiime2 aertifact file (
qza) containing the sequence information. - Metadata table (
tabular) - Truncation length
- Trimming length (optional)
For the paired end workflow the truncation and trimming length for the reverse reads can / has to be given.
- Denoising with
qiime2 dada2 denoise-single/paired - For each of the three outputs (see below) another tool is started to prepare a corresponding qzv file
- representative sequences
qiime2 feature-table tabulate-seqs - denoising statistics
qiime2 metadata tabulate - summary of the feature table
- representative sequences
- representative sequences
- denoising statistics
- summary of the feature table (how many sequences are lost in the corresponding steps)