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performing test run: bsub Rscript ~/determine_cryptic_jumps_and_rates.R ~/single_gene_seq.txt ~/output.txt ~/loss.txt 0 # args[1] == inputfile, args[2] == outfile1, arg[3] == outfile2, # args[4] == 0 ####### make sure the following 3 R packages are installed ####### lpSolve Matrix methods ####### Input ####### format of single_gene_seq.txt file: 1st line: WT group gene info 2nd line: SET2 deleted group gene info 1st column: Gene name 2nd column: Chromosome number 3rd column: CDS start position 4th column: CDS end position 5th column: 1 6th column: strand infomation 7th column and after: reads starting from CDS position 1 to the end of CDS (No nan allowed) ####### Output ####### format of the output.txt file: a list of numbers with the following information: genename, gene length, cryptic initiation site, y, z, MSRLD. formate of the loss.txt file: a list of number with the following information: MSRL value of each position of the gene CDS (except for the starting and ending 150bp respectively). ####### rule of thumb ####### High cryptic initiation: MSRLD >=4 Intermediate cryptic initiation: 2 <= MSRLD < 4 Low cryptic initiation: 0 <= MSRLD < 2 Not quite trustworthy (bad fitting): Min(MSRL) > 15
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