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ANGSD for genotyping and SNPs filtering

This workflow is developped in Louis Bernatchez' lab.

WARNING

The software is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and noninfringement. In no event shall the authors or copyright holders be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of or in connection with the software or the use or other dealings in the software.

Installation

git clone https://github.com/samtools/htslib.git
git clone https://github.com/ANGSD/angsd.git 
cd htslib;make;cd ../angsd ;make HTSSRC=../htslib

Documentation

Trimming

sbatch 00_scripts/01_cutadapt.sh

Demultiplexing

sbatch 00_scripts/02_demultiplexing.sh

Mapping

sbatch 00_scripts/03_mapping_gsnap.sh

Genotyping

Create list of .bam files

ls -1 03_mapped/*.bam > 01_info_files/list_bam

Conduct genotyping

sbatch 00_scripts/04_genotyping.sh

In progress

Citation

Korneliussen T. S., Albrechtsen A. and Nielsen R. 2014. ANGSD: Analysis of Next Generation Sequencing Data. BMC Bioinformatics. DOI: 10.1186/s12859-014-0356-4

Licence

This workflow is licensed under the GPL3 license. See the LICENCE file for more details.

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Pipeline based on ANGSD tools for genotypes calling

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