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Pipeline for analyses of bacterial metatranscriptomics data

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metatranscriptomics_workflow

Pipeline for analyses of Dual-RNAseq metatranscriptomics data in non-model species

WARNING

The software is provided "as is", without warranty of any kind, express or implied, including but not limited to the warranties of merchantability, fitness for a particular purpose and noninfringement. In no event shall the authors or copyright holders be liable for any claim, damages or other liability, whether in an action of contract, tort or otherwise, arising from, out of or in connection with the software or the use or other dealings in the software.

Step-by-step documentation

Trimming

00_scripts/datarmor_jobs/01_trimmomatic_jobs_pe.sh

Meta-transcriptome assembly (microbiome compartment)

qsub 00_scripts/02_trinity_assembly.sh

Assembly statistics

qsub 00_scripts/03_assembly_stats.sh

Meta-transcriptome filtering

qsub 00_scripts/04_longest_isoform.sh
qsub 00_scripts/05_prepare_reference.sh
qsub 00_scripts/datarmor_jobs/06_transcripts_abundance.sh
qsub 00_scripts/07_blastn_conta_host.sh
qsub 00_scripts/08_combine_matrix.sh
qsub 00_scripts/10_bis_filter_rna.sh
qsub 00_scripts/11_fraggenescan.sh
qsub 00_scripts/12_cdhit.sh

Mapping

qsub 00_scripts/13_gmap_index.sh
00_scripts/datarmor_jobs/14_gsnap_mapping_jobs.sh

Counting

00_scripts/datarmor_jobs/15_htseq_count.sh

Annotation

Dependencies

Blast

cd-hit

Fastq-join

SortMeRna

Trimmomatic

Trinity

Notes

This pipeline is adapted from Metatrans.

Authors

Jérémy Le Luyer: jeremy.le.luyer@ifremer.fr

Quentin Schull: quentin.schull@ifremer.fr

Denis Saulnier: denis.saulnier@ifremer.fr

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Pipeline for analyses of bacterial metatranscriptomics data

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