make enriched heatmap which visualizes the enrichment of genomic signals to specific target regions.
R
Latest commit 46cce16 Jan 15, 2017 @jokergoo add rbind.normalizedMatrix

README.md

Build Status codecov bioc bioc

Make Enriched Heatmaps

Enriched heatmap is a special type of heatmap which visualizes the enrichment of genomic signals on specific target regions. It is broadly used to visualize e.g. how histone marks are enriched to specific sites.

There are several tools that can make such heatmap (e.g. ngs.plot or deepTools). Here we implement Enriched heatmap by ComplexHeatmap package. Since this type of heatmap is just a normal heatmap but with some fixed settings, with the functionality of ComplexHeatmap, it would be much easier to customize the heatmap as well as concatenating a list of heatmaps to show correspondance between differnet data sources.

Install

EnrichedHeatmap is available on Bioconductor, you can install it by:

source("http://bioconductor.org/biocLite.R")
biocLite("EnrichedHeatmap") 

If you want the latest version, install it directly from GitHub:

library(devtools)
install_github("jokergoo/EnrichedHeatmap")

Example

Like other tools, the task involves two steps:

  1. Normalize the accosiations between genomic signals and target regions to a matrix.
  2. Draw heatmaps.
mat1 = normalizeToMatrix(H3K4me3, tss, value_column = "coverage", 
    extend = 5000, mean_mode = "w0", w = 50)
mat2 = normalizeToMatrix(meth, tss, value_column = "meth", mean_mode = "absolute",
    extend = 5000, w = 50, empty_value = NA, smooth = TRUE)
partition = kmeans(mat1, centers = 3)$cluster
lgd = Legend(at = c("cluster1", "cluster2", "cluster3"), title = "Clusters", 
    type = "lines", legend_gp = gpar(col = 2:4))
ht_list = Heatmap(partition, col = structure(2:4, names = as.character(1:3)), name = "partition",
              show_row_names = FALSE, width = unit(3, "mm")) +
          EnrichedHeatmap(mat1, col = c("white", "red"), name = "H3K4me3", split = partition, width = 1,
              top_annotation = HeatmapAnnotation(lines = anno_enriched(gp = gpar(col = 2:4))), 
              top_annotation_height = unit(2, "cm"), row_title_rot = 0,
              column_title = "H3K4me3", combined_name_fun = NULL) + 
          EnrichedHeatmap(mat2, name = "methylation", width = 1,
              top_annotation = HeatmapAnnotation(lines = anno_enriched(gp = gpar(col = 2:4))), 
              top_annotation_height = unit(2, "cm"),
              column_title = "Methylation") +
          Heatmap(log2(rpkm+1), col = c("white", "orange"), name = "log2(rpkm+1)", 
              show_row_names = FALSE, width = unit(5, "mm"))
draw(ht_list, main_heatmap = "H3K4me3", gap = unit(c(2, 10, 2), "mm"))

image

Actually you can generate rather complex heatmaps:

image

License

GPL (>= 2)