Instructions for the tutorial are in the in the .Rmd file. We recommend following these steps in the RStudio IDE. Please install the following packages - Seurat, ggdendro, Rmisc, Matrix, MASS, xtable, Matrix.utils, DOSE, reshape2, topGO, randomForest. Additionally, we provide an R script utilities.R which contains some custom functions.
Please download the expression matrices (GSE97930_CerebellarHem_snDrop-seq_UMI_Count_Matrix_08-01-2017.txt.gz, GSE97930_FrontalCortex_snDrop-seq_UMI_Count_Matrix_08-01-2017.txt.gz, GSE97930_VisualCortex_snDrop-seq_UMI_Count_Matrix_08-01-2017.txt.gz) from the following GEO repository and store them in a local folder named Data
https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE97930
Please e-mail any queries to karthik@broadinstitute.org or vm2545@cumc.columbia.edu.