BitPlayer

Last Update 2024/1/18

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BitPlayer is the Matlab app for

1. Recoding with DAQ (NI DAQ device)
2. Video Recording (Point Gray USB Cam) with IMAQ
3. Visual stimuli with PTB3.

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Requirements

• Matlab R2022a
  • GUIs are created using appdesigner
• Windows PC for BitPlayer-DAQ
• Ubuntu for -PTB & -Analysys
• Python (ver 3.8 or 3.9 is compatible for Matlab 2022a)

DAQ

• Windows 11

• Matlab R2022a

  • DAQ toolbox
  • IMAQ toolbox
  • Image Acquisition Toolbox Support Package for Point Grey Hardware

• NI DAQ (USB 6341 (BNC))

• USB3 Camera, FLIR Grasshopper3 USB

• iRecHS2 software (by Dr. Matsuda)

  • Windows 11 (10, 8, 7)
  • Grasshopper3
  • Contec DAC 1604L-LPF
    • 端子台
    • ケーブル

PTB

• Ubuntu 22.04.5 LTS (2022/1/12)
• Matlab R2022a
  • Psychtoolbox3 -> Install for Linux

PTB & DAQ are synchronized by serial communication by monitoring trigger condition

• USB to Serial (RS232) conversion device, sample1, sample2

Analysys

• Developped in Ubuntu 20.04.5 LTS (macOS and Windows not tested)
• Matlab R2022a
• Python 3.8 or 3.9
  • reading meatadata from .oif file OIF2TIF
  • pandas, numpy, scipy

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Visual Stimulation using PTB3

1. Simple spot (full screen)
2. Fine mapping (spot in smaller area)
3. Random size spot
4. Moving bar (fixed bar height: 65deg)
5. Static bar (fixed bar height: 65deg)
6. Moving Spot
7. Sinusoidal, Shifting Grating, Gabor
8. Image Presentation (tested)
9. V1 serach (Coarse & Fine)
10. Decode SC_v1 (RandomCheckerPattern + some figures)
11. 2points, black/white (under development)

How to add new stimulation

1. Add name of new stimulation @./PTB/CheckParams/gui_ini.m
2. Add GUI setting
   1. Add position info in GUI function PatternDropDownValueChanged
   2. Add other GUI settings @./PTB/GUI/Set_StimPattern.m
3. Add procedure of the new stim @./PTB/Stim/VisStimON.m
4. Add text info during stimu presentation ./PTB/GUI/ShowStimInfo.m
5. Save stimulus-specific parameters ./PTB/CheckParams/Get_ParamsSave.m

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DAQ channel configureation (see daq_ini)

1. Recording Eye positino singal from iRecHS2 (anohter WindowsPC)
   1. AI0: Pupil Horizontal movement
   2. AI1: Pupil Verticac movement
   3. AI2: Photo sensor
   4. AI3: **** Used for trigger monitor
   5. AI4: Pupil Size (not good)
2. Video recording of Eye camera (200fps <- 500fps)
3. Recoding locomotion (Rotary encoder)

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Change history

2024/01/18

• Merge update PTB. Add stimuli for Decording SC.

2023/06-12

• Add Image Presentation

2023/03/21-23

• Fix loop structure in DAQ recording.
• Fix bootstrap in Analysys

2022/03/20

• Update Analysys.
• Use version info as txt file.

2022/03/03

• Running test OK for DAQ+PTB and trigger FV10
• Data load is OK for Analsys. Need to make stimulus specific analysys.

2022/02/26

• Developping Analysis app (Analysys + 2P)

2022/01/31

• Change settings of the capturing video from uncompress AVI to motion jpeg AVI.

2022/02/01

• Correct Movie setting.
• Check next number of save-directory.
• Need to repair DAQ save function: SaveData & SaveTimestamps does not save 1st loop...

2022/02/02

• Correct capture setting.
• Delete app.Data and app.TimeStamos. These are temporal file for detecting trigger.
• But, FIFOBuffer can be used.

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