Identification of the essential genes in the M. tuberculosis genome by random transposon mutagenesis

Slides for a talk that I gave a number of times back in 2002-2003, related to the following paper:

Lamichhane G, Zignol M, Blades NJ, Geiman DE, Dougherty A, Broman KW, Bishai WR (2003) A post-genomic method for predicting essential genes at subsaturation levels of mutagenesis: application to Mycobacterium tuberculosis. Proc Natl Acad Sci USA 100:7213-7218