Genome-wide CRISPR interference screen identifies long non-coding RNA loci required for differentiation and pluripotency

This repository contains all of the RNA-seq and CRISPRi screen analysis contained in the above paper. Each analysis lives in a Jupyter notebook. The code runs on Python 3 and requires the following packages:

• matplotlib
• numpy
• pandas
• seaborn
• statsmodels

The CRISPR screen analysis itself is run in R 3.6 and requires the following packages:

• DESeq2
• CRISPhieRmix