Single-nucleus transcriptomics in Area X of male zebra finches (Taeniopygia guttata)

Resources for single nucleus RNA-sequencing data analysis presented in:

Xiao, L., Merullo, D. P., Cao, M., Co, M., Kulkarni, A., Konopka, G., & Roberts, T. F. (2020). Expression of FoxP2 in the basal ganglia regulates vocal motor sequences in the adult songbird. Nature Communications. https://doi.org/10.1038/s41467-021-22918-2

Directories

• MCX20: Raw data for the scrambled control group (CS-shScr) to be loaded with Read10X in Seurat.

• MCX21: Raw data for the experimental FoxP2 knockdown group (CS-shFoxP2) to be loaded with Read10X in Seurat.

• data:
  

  • 01-dataclustering.md: Markdown file of R code to create Seurat objects from raw data. Processed Seurat objects can be downloaded at this link.

  • 02-code: R code to produce all figures (main and supplementary) in the accompanying study.

  • All other files: Processed output from analyses; to be loaded when needed when running code.