pcr-primer-finder

A command-line tool that find optimized PCR-primer for a given fasta file region.

Functionalities

Results of this tool follows somes rules:

• 17 to 25 nucleotids-long primer
• Primer has to end with a C or G
• Both primer need to have the same hybridation temps

To be implemented:

• addition of restriction sites
• addition of promotor

How to use

python main.py -f filename.fasta -b [begining of region interest] -e [end of region of interest]

Example:

python main.py -f test.fasta -b 10 -e 200

(python3)

Screenshot (with test.fasta)