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Error in check.length(gparname) : 'gpar' element 'lwd' must not be length 0 #82

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rekren opened this issue Jul 7, 2022 · 20 comments
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bug Something isn't working

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@rekren
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rekren commented Jul 7, 2022

Hello,

I was happily using clustree package for a while.
However, I received an error message when I run this code;
clustree(x = my_data,prefix = "RNA_snn_res.",exprs = "data",assay = "RNA")

Metadata of my data is below, and everything seems in order from my side.

 colnames(my_data@meta.data)
 [1] "nCount_ADT"                   "nFeature_ADT"                 "orig.ident"                   "nCount_RNA"                  
 [5] "nFeature_RNA"                 "scDblFinder.cluster"          "scDblFinder.class"            "scDblFinder.score"           
 [9] "scDblFinder.weighted"         "scDblFinder.difficulty"       "scDblFinder.cxds_score"       "scDblFinder.mostLikelyOrigin"
[13] "scDblFinder.originAmbiguous"  "id"                           "percent.mt"                   "percent.rb"                  
[17] "condition"                    "source"                       "nCount_SCT"                   "nFeature_SCT"                
[21] "SCT.weight"                   "ADT.weight"                   "wsnn_res.0.5"                 "predicted.celltype.l1.score" 
[25] "predicted.celltype.l1"        "predicted.celltype.l2.score"  "predicted.celltype.l2"        "predicted.celltype.l3.score" 
[29] "predicted.celltype.l3"        "RNA_snn_res.0.1"              "RNA_snn_res.0.2"              "RNA_snn_res.0.3"             
[33] "RNA_snn_res.0.4"              "RNA_snn_res.0.5"              "RNA_snn_res.0.6"              "RNA_snn_res.0.7"             
[37] "RNA_snn_res.0.8"              "RNA_snn_res.0.9"              "RNA_snn_res.1"                "RNA_snn_res.1.1"             
[41] "RNA_snn_res.1.2"              "RNA_snn_res.1.3"              "RNA_snn_res.1.4"              "RNA_snn_res.1.5"             
[45] "seurat_clusters"             

I really appreciate if you can help me to fix the issue leading to 'gpar' element 'lwd' must not be length 0 error.

sessionInfo()
R version 4.2.1 (2022-06-23)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.4 LTS

Matrix products: default
BLAS:   /usr/lib/x86_64-linux-gnu/atlas/libblas.so.3.10.3
LAPACK: /usr/lib/x86_64-linux-gnu/atlas/liblapack.so.3.10.3

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C               LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8    LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C             LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] grid      stats     graphics  grDevices datasets  utils     methods   base     

other attached packages:
 [1] clustree_0.5.0        ggraph_2.0.5          harmony_0.1.0         Rcpp_1.0.8.3          SeuratWrappers_0.3.0 
 [6] SeuratDisk_0.0.0.9020 sp_1.5-0              SeuratObject_4.1.0    Seurat_4.1.1.9002     gridExtra_2.3        
[11] knitr_1.39            forcats_0.5.1         stringr_1.4.0         dplyr_1.0.9           purrr_0.3.4          
[16] readr_2.1.2           tidyr_1.2.0           tibble_3.1.7          ggplot2_3.3.6.9000    tidyverse_1.3.1      

loaded via a namespace (and not attached):
  [1] readxl_1.4.0          backports_1.4.1       plyr_1.8.7            igraph_1.3.2          lazyeval_0.2.2       
  [6] splines_4.2.1         listenv_0.8.0         scattermore_0.8       digest_0.6.29         htmltools_0.5.2      
 [11] viridis_0.6.2         fansi_1.0.3           checkmate_2.1.0       magrittr_2.0.3        tensor_1.5           
 [16] cluster_2.1.3         ROCR_1.0-11           remotes_2.4.2         tzdb_0.3.0            graphlayouts_0.8.0   
 [21] globals_0.15.1        modelr_0.1.8          matrixStats_0.62.0    R.utils_2.12.0        spatstat.sparse_2.1-1
 [26] prettyunits_1.1.1     colorspace_2.0-3      rvest_1.0.2           ggrepel_0.9.1         haven_2.5.0          
 [31] xfun_0.31             rgdal_1.5-32          callr_3.7.0           crayon_1.5.1          jsonlite_1.8.0       
 [36] progressr_0.10.1      spatstat.data_2.2-0   survival_3.3-1        zoo_1.8-10            glue_1.6.2           
 [41] polyclip_1.10-0       gtable_0.3.0          leiden_0.4.2          pkgbuild_1.3.1        future.apply_1.9.0   
 [46] abind_1.4-5           scales_1.2.0          DBI_1.1.3             spatstat.random_2.2-0 miniUI_0.1.1.1       
 [51] viridisLite_0.4.0     xtable_1.8-4          reticulate_1.25       spatstat.core_2.4-4   rsvd_1.0.5           
 [56] bit_4.0.4             htmlwidgets_1.5.4     httr_1.4.3            RColorBrewer_1.1-3    ellipsis_0.3.2       
 [61] ica_1.0-2             farver_2.1.1          R.methodsS3_1.8.2     pkgconfig_2.0.3       uwot_0.1.11          
 [66] dbplyr_2.2.1          deldir_1.0-6          utf8_1.2.2            labeling_0.4.2        tidyselect_1.1.2     
 [71] rlang_1.0.3           reshape2_1.4.4        later_1.3.0           munsell_0.5.0         cellranger_1.1.0     
 [76] tools_4.2.1           cli_3.3.0             generics_0.1.3        broom_1.0.0           ggridges_0.5.3       
 [81] evaluate_0.15         fastmap_1.1.0         goftest_1.2-3         processx_3.6.1        bit64_4.0.5          
 [86] fs_1.5.2              tidygraph_1.2.1       fitdistrplus_1.1-8    RANN_2.6.1            pbapply_1.5-0        
 [91] future_1.26.1         nlme_3.1-158          mime_0.12             R.oo_1.25.0           xml2_1.3.3           
 [96] hdf5r_1.3.5           compiler_4.2.1        rstudioapi_0.13       curl_4.3.2            plotly_4.10.0        
[101] png_0.1-7             spatstat.utils_2.3-1  reprex_2.0.1          tweenr_1.0.2          stringi_1.7.6        
[106] ps_1.7.1              rgeos_0.5-9           lattice_0.20-45       Matrix_1.4-1          vctrs_0.4.1          
[111] pillar_1.7.0          lifecycle_1.0.1       BiocManager_1.30.18   spatstat.geom_2.4-0   lmtest_0.9-40        
[116] RcppAnnoy_0.0.19      data.table_1.14.2     cowplot_1.1.1         irlba_2.3.5           httpuv_1.6.5         
[121] patchwork_1.1.1       R6_2.5.1              promises_1.2.0.1      renv_0.15.5           KernSmooth_2.23-20   
[126] parallelly_1.32.0     codetools_0.2-18      MASS_7.3-57           assertthat_0.2.1      rprojroot_2.0.3      
[131] withr_2.5.0           sctransform_0.3.3     mgcv_1.8-40           parallel_4.2.1        hms_1.1.1            
[136] rpart_4.1.16          Rtsne_0.16            ggforce_0.3.3         shiny_1.7.1           lubridate_1.8.0      
@denvercal1234GitHub
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denvercal1234GitHub commented Jul 12, 2022

Same issue observed for my data. @rekren did you solve it? @lazappi - Any advice for us? Thank you so much in advance.

@denvercal1234GitHub
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So I tested.

Different versions of the package were not the issue.

When I analyze the data on our server instead of the local computer, the error went away.

Not sure the underlying issue still, but I hope it helps.

@lazappi lazappi added the bug Something isn't working label Jul 18, 2022
@lazappi
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lazappi commented Jul 18, 2022

This sounds like it might be a dependency issue somewhere. Can you please post the full error message (the output from traceback() might also be helpful)?

@denvercal1234GitHub
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Thank you so much for your response.

Below is the full error message:

Screen Shot 2022-07-18 at 9 45 02 AM

And traceback() output:

Screen Shot 2022-07-18 at 9 45 25 AM

@lazappi
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lazappi commented Jul 18, 2022

This looks like it is a problem rendering the plot rather than something inside {clustree}. Can you try the default settings without setting any arguments (except prefix)? Do you have issues making any other plots or just this one? It might be useful if you are able to share the plot object.

Also small note, it's generally better to include code as text using the `code` syntax rather than insert images.

@denvercal1234GitHub
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denvercal1234GitHub commented Jul 18, 2022

Thank you for your advice. Please see below.

I do not have any issue making other plots using other packages. Only all the plotting functions of the clustree package that had this same error.

This error was not specific to my Seurat object, because here I used pbmc3k object available from SeuratData, and the same issue occured.

SeuratData::InstallData("pbmc3k")
pbmc3k.final$groups <- sample(c("group1", "group2"), size = ncol(pbmc3k.final), replace = TRUE)
colnames(pbmc3k.final@meta.data)
clustree(pbmc3k.final, prefix = "RNA_snn_res.")

> colnames(pbmc3k.final@meta.data)
 [1] "orig.ident"         "nCount_RNA"         "nFeature_RNA"       "seurat_annotations" "percent.mt"        
 [6] "RNA_snn_res.0.5"    "seurat_clusters"    "groups"             "RNA_snn_res.1"      "RNA_snn_res.1.5"   
> clustree(pbmc3k.final, prefix = "RNA_snn_res.") 
Error in check.length(gparname) : 
  'gpar' element 'lwd' must not be length 0
> traceback()
16: stop(gettextf("'gpar' element '%s' must not be length 0", gparname), 
        domain = NA)
15: check.length(gparname)
14: numnotnull("lwd")
13: validGP(list(...))
12: gpar(col = guide$ticks.colour, lwd = guide$ticks.linewidth * 
        .pt, lineend = "butt")
11: grob(x0 = x0, y0 = y0, x1 = x1, y1 = y1, arrow = arrow, name = name, 
        gp = gp, vp = vp, cl = "segments")
10: segmentsGrob(x0 = x0, y0 = y0, x1 = x1, y1 = y1, default.units = "cm", 
        gp = gpar(col = guide$ticks.colour, lwd = guide$ticks.linewidth * 
            .pt, lineend = "butt"))
9: guide_gengrob.colorbar(X[[i]], ...)
8: FUN(X[[i]], ...)
7: lapply(gdefs, guide_gengrob, theme)
6: guides_gengrob(gdefs, theme)
5: build_guides(plot$scales, plot$layers, plot$mapping, position, 
       theme, plot$guides, plot$labels)
4: ggplot_gtable.ggplot_built(data)
3: ggplot_gtable(data)
2: print.ggplot(x)
1: (function (x, ...) 
   UseMethod("print"))(x)

@lazappi
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lazappi commented Jul 19, 2022

Can you please also post the output of sessionInfo()?

@denvercal1234GitHub
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> sessionInfo()
R version 4.2.0 (2022-04-22)
Platform: x86_64-apple-darwin17.0 (64-bit)
Running under: macOS Monterey 12.4

Matrix products: default
LAPACK: /Library/Frameworks/R.framework/Versions/4.2/Resources/lib/libRlapack.dylib

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
[1] leiden_0.4.2       igraph_1.3.3       reticulate_1.25    sp_1.5-0           SeuratObject_4.1.0 Seurat_4.1.1.9002 

loaded via a namespace (and not attached):
  [1] Rtsne_0.16            colorspace_2.0-3      deldir_1.0-6          ellipsis_0.3.2        ggridges_0.5.3        rprojroot_2.0.3      
  [7] fs_1.5.2              gridtext_0.1.4        ggtext_0.1.1          rstudioapi_0.13       spatstat.data_2.2-0   listenv_0.8.0        
 [13] remotes_2.4.2         ggrepel_0.9.1         SeuratData_0.2.2      fansi_1.0.3           xml2_1.3.3            codetools_0.2-18     
 [19] splines_4.2.0         cachem_1.0.6          pkgload_1.3.0         polyclip_1.10-0       jsonlite_1.8.0        ica_1.0-3            
 [25] cluster_2.1.3         png_0.1-7             rgeos_0.5-9           uwot_0.1.11           shiny_1.7.1           sctransform_0.3.3    
 [31] spatstat.sparse_2.1-1 BiocManager_1.30.18   compiler_4.2.0        httr_1.4.3            assertthat_0.2.1      Matrix_1.4-1         
 [37] fastmap_1.1.0         lazyeval_0.2.2        cli_3.3.0             later_1.3.0           prettyunits_1.1.1     htmltools_0.5.2      
 [43] tools_4.2.0           gtable_0.3.0          glue_1.6.2            RANN_2.6.1            reshape2_1.4.4        dplyr_1.0.9          
 [49] rappdirs_0.3.3        Rcpp_1.0.9            scattermore_0.8       vctrs_0.4.1           nlme_3.1-158          progressr_0.10.1     
 [55] lmtest_0.9-40         spatstat.random_2.2-0 stringr_1.4.0         ps_1.7.1              globals_0.15.1        mime_0.12            
 [61] miniUI_0.1.1.1        lifecycle_1.0.1       irlba_2.3.5           devtools_2.4.3        goftest_1.2-3         future_1.26.1        
 [67] MASS_7.3-58           zoo_1.8-10            scales_1.2.0          spatstat.core_2.4-4   promises_1.2.0.1      spatstat.utils_2.3-1 
 [73] parallel_4.2.0        RColorBrewer_1.1-3    memoise_2.0.1         pbapply_1.5-0         gridExtra_2.3         ggplot2_3.3.6.9000   
 [79] rpart_4.1.16          stringi_1.7.8         pkgbuild_1.3.1        rlang_1.0.4           pkgconfig_2.0.3       matrixStats_0.62.0   
 [85] lattice_0.20-45       ROCR_1.0-11           purrr_0.3.4           tensor_1.5            patchwork_1.1.1       htmlwidgets_1.5.4    
 [91] processx_3.7.0        cowplot_1.1.1         tidyselect_1.1.2      here_1.0.1            parallelly_1.32.0     RcppAnnoy_0.0.19     
 [97] plyr_1.8.7            magrittr_2.0.3        R6_2.5.1              generics_0.1.3        DBI_1.1.3             pillar_1.7.0         
[103] mgcv_1.8-40           fitdistrplus_1.1-8    survival_3.3-1        abind_1.4-5           tibble_3.1.7          future.apply_1.9.0   
[109] crayon_1.5.1          KernSmooth_2.23-20    utf8_1.2.2            spatstat.geom_2.4-0   plotly_4.10.0         usethis_2.1.6        
[115] grid_4.2.0            data.table_1.14.2     callr_3.7.1           digest_0.6.29         xtable_1.8-4          tidyr_1.2.0          
[121] httpuv_1.6.5          munsell_0.5.0         viridisLite_0.4.0     sessioninfo_1.2.2  

@rekren
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rekren commented Jul 21, 2022

This looks like it is a problem rendering the plot rather than something inside {clustree}. Can you try the default settings without setting any arguments (except prefix)? Do you have issues making any other plots or just this one? It might be useful if you are able to share the plot object.

Also small note, it's generally better to include code as text using the `code` syntax rather than insert images.

Thanks for looking into this issue @lazappi !

I was using the Cairo graphics device backend setting and switched to default but the outcome didn't change. Just in case I tried all other backend settings and downgrading the installed ggplot from its developer version (ggplot2_3.3.6.9000) to cran version (ggplot2_3.3.6) didn't work either.
Still Error in check.length(gparname) : 'gpar' element 'lwd' must not be length 0

When I check traceback() the output is exactly same with @denvercal1234GitHub 's.

@lazappi
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lazappi commented Jul 21, 2022

It looks like you are both using a development version of {ggplot2}. Can you please try with the current release version (v3.3.6)?

@rekren
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rekren commented Jul 21, 2022

It looks like you are both using a development version of {ggplot2}. Can you please try with the current release version (v3.3.6)?

I also used the current release version (v3.3.6), and the outcome didn't change.

@lazappi
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lazappi commented Jul 21, 2022

Hmmm...ok. I'm running out of obvious ideas for where this might be coming from. I think it will take a bit more investigation to pin down. Unfortunately I won't have time to look into it too much for the next few weeks.

@rekren
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rekren commented Jul 29, 2022

My issue is spontaneously resolved, presumably thanks to the update of one of the graphics/matrix dependency packages.
Below is my session info which runs the clustree() flawlessly for the same dataset and also for "pbmc_small" test data of Seurat package.

sessionInfo()
R version 4.2.1 (2022-06-23)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 20.04.4 LTS

Matrix products: default
BLAS:   /usr/lib/x86_64-linux-gnu/atlas/libblas.so.3.10.3
LAPACK: /usr/lib/x86_64-linux-gnu/atlas/liblapack.so.3.10.3

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C               LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8     LC_MONETARY=en_US.UTF-8   
 [6] LC_MESSAGES=en_US.UTF-8    LC_PAPER=en_US.UTF-8       LC_NAME=C                  LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] grid      stats     graphics  grDevices datasets  utils     methods   base     

other attached packages:
 [1] clustree_0.5.0        ggraph_2.0.5          HGNChelper_0.8.1      harmony_0.1.0         Rcpp_1.0.9            SeuratWrappers_0.3.0  SeuratDisk_0.0.0.9020
 [8] sp_1.5-0              SeuratObject_4.1.0    Seurat_4.1.1.9002     gridExtra_2.3         knitr_1.39            forcats_0.5.1         stringr_1.4.0        
[15] dplyr_1.0.9           purrr_0.3.4           readr_2.1.2           tidyr_1.2.0           tibble_3.1.8          ggplot2_3.3.6         tidyverse_1.3.2      

loaded via a namespace (and not attached):
  [1] ggprism_1.0.3.9000          scattermore_0.8             R.methodsS3_1.8.2           bit64_4.0.5                 irlba_2.3.5                
  [6] DelayedArray_0.22.0         R.utils_2.12.0              data.table_1.14.2           rpart_4.1.16                RCurl_1.98-1.7             
 [11] doParallel_1.0.17           generics_0.1.3              BiocGenerics_0.42.0         cowplot_1.1.1               RANN_2.6.1                 
 [16] future_1.27.0               bit_4.0.4                   tzdb_0.3.0                  spatstat.data_2.2-0         xml2_1.3.3                 
 [21] lubridate_1.8.0             httpuv_1.6.5                SummarizedExperiment_1.26.1 assertthat_0.2.1            isoband_0.2.5              
 [26] viridis_0.6.2               gargle_1.2.0                xfun_0.31                   hms_1.1.1                   jquerylib_0.1.4            
 [31] evaluate_0.15               promises_1.2.0.1            fansi_1.0.3                 dbplyr_2.2.1                readxl_1.4.0               
 [36] igraph_1.3.4                DBI_1.1.3                   htmlwidgets_1.5.4           spatstat.geom_2.4-0         googledrive_2.0.0          
 [41] stats4_4.2.1                paletteer_1.4.0             ellipsis_0.3.2              RSpectra_0.16-1             ggpubr_0.4.0               
 [46] backports_1.4.1             deldir_1.0-6                MatrixGenerics_1.8.1        vctrs_0.4.1                 SingleCellExperiment_1.18.0
 [51] Biobase_2.56.0              remotes_2.4.2               ROCR_1.0-11                 abind_1.4-5                 cachem_1.0.6               
 [56] withr_2.5.0                 ggforce_0.3.3               progressr_0.10.1            checkmate_2.1.0             rgdal_1.5-32               
 [61] sctransform_0.3.3           dittoSeq_1.8.1              goftest_1.2-3               cluster_2.1.3               lazyeval_0.2.2             
 [66] crayon_1.5.1                hdf5r_1.3.5                 pkgconfig_2.0.3             labeling_0.4.2              tweenr_1.0.2               
 [71] GenomeInfoDb_1.32.2         nlme_3.1-158                vipor_0.4.5                 rlang_1.0.4                 globals_0.15.1             
 [76] lifecycle_1.0.1             miniUI_0.1.1.1              modelr_0.1.8                rsvd_1.0.5                  ggrastr_1.0.1              
 [81] cellranger_1.1.0            polyclip_1.10-0             matrixStats_0.62.0          lmtest_0.9-40               Matrix_1.4-1               
 [86] carData_3.0-5               zoo_1.8-10                  reprex_2.0.1                beeswarm_0.4.0              ggridges_0.5.3             
 [91] GlobalOptions_0.1.2         googlesheets4_1.0.0         pheatmap_1.0.12             png_0.1-7                   viridisLite_0.4.0          
 [96] rjson_0.2.21                bitops_1.0-7                R.oo_1.25.0                 KernSmooth_2.23-20          shape_1.4.6                
[101] parallelly_1.32.1           spatstat.random_2.2-0       rstatix_0.7.0               S4Vectors_0.34.0            ggsignif_0.6.3             
[106] scales_1.2.0                hexbin_1.28.2               magrittr_2.0.3              plyr_1.8.7                  ica_1.0-3                  
[111] zlibbioc_1.42.0             compiler_4.2.1              RColorBrewer_1.1-3          clue_0.3-61                 fitdistrplus_1.1-8         
[116] snakecase_0.11.0            cli_3.3.0                   XVector_0.36.0              listenv_0.8.0               patchwork_1.1.1            
[121] pbapply_1.5-0               MASS_7.3-58                 mgcv_1.8-40                 tidyselect_1.1.2            stringi_1.7.8              
[126] highr_0.9                   yaml_2.3.5                  ggrepel_0.9.1               sass_0.4.2                  scCustomize_0.7.0          
[131] tools_4.2.1                 future.apply_1.9.0          parallel_4.2.1              circlize_0.4.15             rstudioapi_0.13            
[136] foreach_1.5.2               janitor_2.1.0               farver_2.1.1                Rtsne_0.16                  digest_0.6.29              
[141] BiocManager_1.30.18         rgeos_0.5-9                 shiny_1.7.2                 GenomicRanges_1.48.0        car_3.1-0                  
[146] broom_1.0.0                 later_1.3.0                 RcppAnnoy_0.0.19            httr_1.4.3                  rsconnect_0.8.27           
[151] ComplexHeatmap_2.12.0       colorspace_2.0-3            rvest_1.0.2                 fs_1.5.2                    tensor_1.5                 
[156] reticulate_1.25             IRanges_2.30.0              splines_4.2.1               uwot_0.1.11                 rematch2_2.1.2             
[161] spatstat.utils_2.3-1        graphlayouts_0.8.0          renv_0.15.5                 plotly_4.10.0               xtable_1.8-4               
[166] jsonlite_1.8.0              tidygraph_1.2.1             R6_2.5.1                    pillar_1.8.0                htmltools_0.5.3            
[171] mime_0.12                   glue_1.6.2                  fastmap_1.1.0               codetools_0.2-18            utf8_1.2.2                 
[176] lattice_0.20-45             bslib_0.4.0                 spatstat.sparse_2.1-1       ggbeeswarm_0.6.0            leiden_0.4.2               
[181] zip_2.2.0                   colorway_0.2.0              openxlsx_4.2.5              survival_3.3-1              rmarkdown_2.14             
[186] munsell_0.5.0               GetoptLong_1.0.5            GenomeInfoDbData_1.2.8      iterators_1.0.14            haven_2.5.0                
[191] reshape2_1.4.4              gtable_0.3.0                spatstat.core_2.4-4        

@rekren rekren closed this as completed Jul 29, 2022
@yoda-vid
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I stumbled upon this error as well, which eventually resolved after updating ggraph from 2.0.5 to 2.1.0. Not sure exactly how this works since the ggraph version appears unchanged at 2.0.5 in @rekren's posts above, so maybe it was some combination of package versions.

@lazappi
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lazappi commented Nov 21, 2022

Ok, thanks for the update! If anyone is able to pin down exactly which package/version combinations are the issue I'm happy to update the dependencies but without that I'm not sure there is much I can do.

@jmvarberg
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+1 for @yoda-vid recommendation - updating from ggraph 2.0.6 to 2.1.0 resolved this issue for me today as well. Used with SeuratObject_4.1.3, Seurat_4.3.0, and clustree_0.5.0.

@etiennedanis
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+1 for @yoda-vid recommendation as well. Updating ggraph package from 2.0.6 to 2.1.0 worked for me too.
( install.packages("ggraph", version = "2.1.0") and restarting R )

@tqh003
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tqh003 commented Mar 16, 2023

+1 for @yoda-vid recommendation as well. Updating ggraph package from 2.0.5 to 2.1.0 worked for me too.

@HanlinYin
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+1 for @yoda-vid recommendation as well. I reinstalled clustree and update ggraph from 2.0.6 to 2.1.0. Works well. Thanks a lot.

@utkarsh0493
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+1 @yoda-vid updating ggraph from 2.0.5 to 2.1.0 worked for me as well.
Thanks alot.

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