Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

Does it fit for 1kb to 2kb read length data #20

Open
arpit20328 opened this issue Apr 20, 2024 · 4 comments
Open

Does it fit for 1kb to 2kb read length data #20

arpit20328 opened this issue Apr 20, 2024 · 4 comments

Comments

@arpit20328
Copy link

I am doing 16S rna amplicon based ONT sequencing.

My target region is only of 1kb to 2kb in length.

Per sample reads is around 50K.

Will HEERO give me good error filtered estimates ?

@dominikstanojevic
Copy link
Member

Hello Arpit,

At the moment, HERRO does not work on short sequences. However, we may add support for them in the future.

@ramiroricardo
Copy link

Great... hoping you will!

@yzhang-github-pub
Copy link

@dominikstanojevic please do add support for shorter reads. There are a lot of applications where short reads in lower kb range are long enough. Thanks!

@avilella
Copy link

avilella commented Aug 1, 2024

I am echoing the same request above: in my case, I would like to dorado correct single-cell RNAseq that are split in buckets containing the same barcode or barcode+UMI, which guarantees they should be sequencing the same original molecule.

Bests

Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
None yet
Projects
None yet
Development

No branches or pull requests

5 participants