XML syntax fix EP-0505058-B1

lfoppiano · Sep 8, 2017 · 0e576c5 · 0e576c5
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commit 0e576c5
Showing 1 changed file with 3 additions and 3 deletions.
diff --git a/resources/dataset/quantities/corpus/xml/staging/EP-0505058-B1.tei.xml b/resources/dataset/quantities/corpus/xml/staging/EP-0505058-B1.tei.xml
@@ -308,7 +308,7 @@ wherein Z has the same significance as described above, preferably in the presen
 		<p xml:id="_6a299">Next, the immunoregulating activity, activity of inhibiting bone absorption and acute toxicity of Compound (I) are described by referring to test examples. </p>
 		<head xml:id="_69385">Test Example 1 Plaque Forming Cell Assay</head>
 		<p xml:id="_dad17">The methods developed by Jerne [Science, 140, 405 (1963)] and Yamamoto, et al[Drugs. Exptl. Clin. Res., 8, 5 (1982)] were modified for plaque forming cell assay. </p>
-		<p xml:id="_d6730">That is, Balb/c strain male mice (age of 7 weeks, Charles River Japan Inc.) were sensitized with <measure type="value"><num>1 x 108</num></measure> sheep red blood cells (Bio Test Research Institute) and the spleen was extirpated on the <measure type="list"><num>sixth</num> or <num>seventh</num> <measure type="TIME" unit="day">day</measure></measure>. The cells obtained from the spleen were treated with ACT solution (Tris-ammonium chloride isotonic buffer) to remove red blood cells. The cells were washed <measure type="value"><num>three</num></measure> times with RPMI1640 medium (Nissui Pharmaceutical Co.). The cells (<measure type="value"><num>1 x107</num></measure>) were incubated in RPMI-1640 medium containing <measure type="value"><num>10</num><measure type="FRACTION" unit="%">%</measure></measure> calf fetal serum (Gibco Co.), <measure type="value"><num>50</num> <measure type="DENSITY" unit="µg.ml⁻¹">µg/ml</measure></measure> streptomycin, <measure type="value"><num>50</num> <measure type="DENSITY" unit="IU.ml⁻¹">IU/ml</measure></measure> of penicillin, 2-mercaptoethanol (<measure type="value"><num>5 x 10-5</num> <measure type="DENSITY" unit="molarity">M</measure></measure>), sheep red blood cells (<measure type="value"><num>5 x 106</num></measure> cells) and a test compound dissolved in dimethyl sulfoxide supplied on a microculture plate (NUNC Co., 24 wells) in a carbon dioxide gas incubator (TABAI ESPEC CORP) at <measure type="value"><num>37</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="value"><num>5</num> <measure type="TIME" unit="day">days</measure></measure>. </p><p xml:id="_a8041">After completion of the incubation, the cells were transferred to a plastic test tube and centrifuged at <measure type="value"><num>2000<num> <measure type="FREQUENCY" unit="rpm">rpm</measure></measure>. After the supernatant was removed, the cells were resuspended in <measure type="value"><num>1</num> <measure type="MASS" unit="ml">ml</measure></measure> of RPMI-1640 medium. The cell suspension was sealed in a Cunnigham chamber (Takahashi Giken Co.) together with sheep red blood cells and guinea pig complement (Cedarlane Research Institute) according to the method of Cunnigham [Immunology, 14, 599 (1968)] and incubated at <measure type="value"><num>37</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="interval"><num atLeast="1">1</num> to <num atMost="2">2</num> <measure type="TIME" unit="h">hours</measure></measure>. Direct plaque forming cell (PFC) count was counted. </p>
+		<p xml:id="_d6730">That is, Balb/c strain male mice (age of 7 weeks, Charles River Japan Inc.) were sensitized with <measure type="value"><num>1 x 108</num></measure> sheep red blood cells (Bio Test Research Institute) and the spleen was extirpated on the <measure type="list"><num>sixth</num> or <num>seventh</num> <measure type="TIME" unit="day">day</measure></measure>. The cells obtained from the spleen were treated with ACT solution (Tris-ammonium chloride isotonic buffer) to remove red blood cells. The cells were washed <measure type="value"><num>three</num></measure> times with RPMI1640 medium (Nissui Pharmaceutical Co.). The cells (<measure type="value"><num>1 x107</num></measure>) were incubated in RPMI-1640 medium containing <measure type="value"><num>10</num><measure type="FRACTION" unit="%">%</measure></measure> calf fetal serum (Gibco Co.), <measure type="value"><num>50</num> <measure type="DENSITY" unit="µg.ml⁻¹">µg/ml</measure></measure> streptomycin, <measure type="value"><num>50</num> <measure type="DENSITY" unit="IU.ml⁻¹">IU/ml</measure></measure> of penicillin, 2-mercaptoethanol (<measure type="value"><num>5 x 10-5</num> <measure type="DENSITY" unit="molarity">M</measure></measure>), sheep red blood cells (<measure type="value"><num>5 x 106</num></measure> cells) and a test compound dissolved in dimethyl sulfoxide supplied on a microculture plate (NUNC Co., 24 wells) in a carbon dioxide gas incubator (TABAI ESPEC CORP) at <measure type="value"><num>37</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="value"><num>5</num> <measure type="TIME" unit="day">days</measure></measure>. </p><p xml:id="_a8041">After completion of the incubation, the cells were transferred to a plastic test tube and centrifuged at <measure type="value"><num>2000</num> <measure type="FREQUENCY" unit="rpm">rpm</measure></measure>. After the supernatant was removed, the cells were resuspended in <measure type="value"><num>1</num> <measure type="MASS" unit="ml">ml</measure></measure> of RPMI-1640 medium. The cell suspension was sealed in a Cunnigham chamber (Takahashi Giken Co.) together with sheep red blood cells and guinea pig complement (Cedarlane Research Institute) according to the method of Cunnigham [Immunology, 14, 599 (1968)] and incubated at <measure type="value"><num>37</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="interval"><num atLeast="1">1</num> to <num atMost="2">2</num> <measure type="TIME" unit="h">hours</measure></measure>. Direct plaque forming cell (PFC) count was counted. </p>
 		<p xml:id="_d1901">A rate of inhibiting antibody production by the test compound was determined by the following equation. Inhibition rate (%) = A - BA x 100 A :PFC count in the absence of test compound (dimethylsulfoxide alone) B :PFC count in the presence of test compound </p>
 		<p xml:id="_daaa2">The results are shown in Table 3.
 			<figure><table cols="4"><row><cell>Compound No. </cell><cell>Concentration (M) </cell><cell>Direct PFC Count (mean ± S.E.M.) </cell><cell>Inhibition Rate (%) </cell></row><row><cell>Control</cell><cell/><cell>5023 ± 38 </cell></row><row><cell>3</cell><cell>10-4</cell><cell>101 ± 76</cell><cell>98.0 </cell></row><row><cell/><cell>10-5</cell><cell>59 ± 38</cell><cell>98.8 </cell></row><row><cell>4</cell><cell>10-4</cell><cell>336 ± 124</cell><cell>93.3 </cell></row><row><cell/><cell>10-5</cell><cell>395 ± 52</cell><cell>92.1 </cell></row><row><cell>5</cell><cell>10-4</cell><cell>109 ± 77</cell><cell>97.8 </cell></row><row><cell/><cell>10-5</cell><cell>227 ± 131</cell><cell>95.5 </cell></row><row><cell>6</cell><cell>10-4</cell><cell>42 ± 29</cell><cell>99.2 </cell></row><row><cell/><cell>10-5</cell><cell>59 ± 29</cell><cell>98.8 </cell></row></table></figure>
@@ -319,7 +319,7 @@ wherein Z has the same significance as described above, preferably in the presen
 			<figure><table cols="3"><row><cell>Compound No. </cell><cell>Concentration (µM) </cell><cell>Inhibition Rate (%) </cell></row><row><cell>1</cell><cell>100</cell><cell>-1 </cell></row><row><cell>2</cell><cell>100</cell><cell>51 </cell></row><row><cell>3</cell><cell>10</cell><cell>141 </cell></row><row><cell>4</cell><cell>10</cell><cell>58 </cell></row><row><cell>5</cell><cell>10</cell><cell>53 </cell></row><row><cell>6</cell><cell>10</cell><cell>38 </cell></row><row><cell>7</cell><cell>10</cell><cell>32 </cell></row><row><cell>8</cell><cell>10</cell><cell>18 </cell></row></table></figure>
 		</p>
 		<head xml:id="_b1c62">Test Example 3 Acute toxicity test</head>
-		<p xml:id="_b7e8b">A test compound was orally administered to three dd-strain male mice weighing <measure type="interval"><num type="base">20</num> ± <num type="range">1</num> <measure type="MASS" unit="g">g</measure></measure>. The minimum lethal dose (MLD) was determined by observing the mortality for <measure type="value"><num>7</num> <measure type="TIME" unit="day">days</measure></measure> after the administration. </p><p xml:id="_d10b5">The results are shown in Table 5.
+		<p xml:id="_b7e8b">A test compound was orally administered to <measure type="value"><num>three</num></measure> dd-strain male mice weighing <measure type="interval"><num type="base">20</num> ± <num type="range">1</num> <measure type="MASS" unit="g">g</measure></measure>. The minimum lethal dose (MLD) was determined by observing the mortality for <measure type="value"><num>7</num> <measure type="TIME" unit="day">days</measure></measure> after the administration. </p><p xml:id="_d10b5">The results are shown in Table 5.
 			<figure><table cols="2"><row><cell>Compound No. </cell><cell>MLD (mg/kg) </cell></row><row><cell>4</cell><cell>&gt; 300 </cell></row><row><cell>7</cell><cell>&gt; 300 </cell></row></table></figure>
 		</p>
 		<p xml:id="_37c9c">Compound (I) or a pharmaceutically acceptable salt thereof may be used as it is, or in various pharmaceutical forms. The pharmaceutical composition of the present invention can be prepared by uniformly mixing an effective amount of Compound (I) or a pharmaceutically acceptable salt thereof as the active ingredient with pharmaceutically acceptable carriers. The pharmaceutical compositions are desirably in a single dose unit suited for oral or parenteral administration. </p>
@@ -332,7 +332,7 @@ wherein Z has the same significance as described above, preferably in the presen
 		<p xml:id="_72938">A mixture of <measure type="value"><num>2.43</num> <measure type="MASS" unit="g">g</measure></measure> (<measure type="value"><num>10.2</num> <measure type="AMOUNT_OF_SUBSTANCE" unit="mmol">mmols</measure></measure>) of ethyl 4,5-dihydro-7-hydroxy-5-oxothieno[3,2-b]pyridine-6-carboxylate [J. Chem. Res. (S), 6 (1980); J. Chem. Res. (M), 113 (1980)], <measure type="value"><num>1.00</num> <measure type="MASS">g</measure></measure> (<measure type="value"><num>10.6</num> <measure type="AMOUNT_OF_SUBSTANCE" unit="mmol">mmols</measure></measure>) of <measure type="value"><num>3</num>-aminopyridine, <measure type="value"><num>50</num> <measure type="VOLUME" unit="ml">ml</measure></measure> of xylene and <measure type="value"><num>10</num> <measure type="VOLUME">ml</measure></measure> of dimethylformamide was heated at <measure type="value"><num>140</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="value"><num value="1">an</num> <measure type="TIME" unit="h">hour</measure></measure>. After completion of the reaction, insoluble matters were filtered and recrystallized from dimethylformamide to give <measure type="value"><num>1.56</num> <measure type="MASS">g</measure></measure> (yield: <measure type="value"><num>54</num><measure type="FRACTION" unit="%">%</measure></measure>) of Compound 1.
 			<figure><table cols="4"><row><cell>Elemental analysis: C13H9N3O3S </cell></row><row><cell>Calcd. (%) :</cell><cell>C 54.35,</cell><cell>H 3.16,</cell><cell>N 14.63 </cell></row><row><cell>Found (%) :</cell><cell>C 54.11,</cell><cell>H 2.85,</cell><cell>N 14.48 </cell></row></table></figure>
 IR (KBr) <measure type="list"><measure type="ENERGY" unit="cm^-1">cm-1</measure>:<num>3450</num>(br), <num>1638</num>, <num>1594</num>, <num>1547</num>, <num>1480</num>, <num>1408</num>, <num>1364</num>, <num>1264</num>, <num>1228</num>, <num>799</num>, <num>761</num></measure> 
-NMR (CF3 CO2 D) δ <measure type="list">(<measure type="CONCENTRATION" unit="ppm">ppm</measure>):<num>9.79</num>(1H, s), <num>8.81</num>(1H, d, J= <measure type="value"><num>8.8</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>8.63</num>(1H, d, J=<measure type="value"><num>5.1</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>8.15</num>(1H, m), <num>8.10</num> (1H, d, J=<measure type="value"><num>5.4</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>7.28</num>(1H, d, J=<measure type="value"><num>5.4</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>)</measure></p>
+NMR (CF3 CO2 D) δ <measure type="list">(<measure type="CONCENTRATION" unit="ppm">ppm</measure>):<num>9.79</num>(1H, s), <num>8.81</num>(1H, d, J= <measure type="value"><num>8.8</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>8.63</num>(1H, d, J=<measure type="value"><num>5.1</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>8.15</num>(1H, m), <num>8.10</num> (1H, d, J=<measure type="value"><num>5.4</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>), <num>7.28</num>(1H, d, J=<measure type="value"><num>5.4</num><measure type="FREQUENCY" unit="Hz">Hz</measure></measure>)</measure></measure></p>
 		<head xml:id="_9a299">Example 2</head>
 		<head xml:id="_e2133">4,5-Dihydro-7-hydroxy-5-oxo-N-(4-pyridyl)thieno[3,2-b]pyridine-6-carboxamide (Compound 2)</head>
 		<p xml:id="_2196c">A mixture of <measure type="value"><num>2.48</num> <measure type="MASS" unit="g">g</measure></measure> (<measure type="value"><num>10.4</num> <measure type="AMOUNT_OF_SUBSTANCE" unit="mmol">mmols</measure></measure>) of ethyl 4,5-dihydro-7-hydroxy-5-oxothieno[3,2-b]pyridine-6-carboxylate [J. Chem. Res. (S), 6 (1980); J. Chem. Res. (M), 113 (1980)], <measure type="value"><num>1.01</num> <measure type="MASS">g</measure></measure> (<measure type="value"><num>10.7</num> <measure type="AMOUNT_OF_SUBSTANCE" unit="mmol">mmols</measure></measure>) of 4-aminopyridine, <measure type="value"><num>50</num> <measure type="VOLUME" unit="ml">ml</measure></measure> of xylene and <measure type="value"><num>10</num> <measure type="VOLUME">ml</measure></measure> of dimethylformamide was heated at <measure type="value"><num>140</num><measure type="TEMPERATURE" unit="°C">°C</measure></measure> for <measure type="value"><num value="1">an</num> <measure type="TIME" unit="h">hour</measure></measure>. After completion of the reaction, insoluble matters were filtered and tritylated with dimethylformamide with heating to give <measure type="value"><num>1.99</num> <measure type="MASS" unit="g">g</measure></measure> (yield: <measure type="value"><num>67</num><measure type="FRACTION" unit="%">%</measure></measure>) of Compound 2.